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    Methods for using a thermostable phytase in ethanol production
    1.
    发明授权
    Methods for using a thermostable phytase in ethanol production 有权
    在乙醇生产中使用热稳定性植酸酶的方法

    公开(公告)号：US08936924B2

    公开(公告)日：2015-01-20

    申请号：US14032578

    申请日：2013-09-20

    申请人： Verenium Corporation

    发明人： Arne Solbak ,  Brian Steer ,  Mark Dycaico ,  Katie A. Kline ,  Axel Trefzer ,  Thomas Todaro ,  Fatima El-Farrah ,  Alberto Alvarado ,  Gerhard Frey

    IPC分类号： C12P7/02 ,  C12P7/22 ,  C12P7/06 ,  C12P7/10 ,  C12N9/16

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23L33/18 ,  A23V2002/00 ,  C12P7/10 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072 ,  Y02E50/16 ,  Y02E50/17

    摘要： A method for using a thermostable phytase for eliminating or reducing phytic acid or salts of phytic acid in an alcohol production process is disclosed. The phytase can be added anywhere in the alcohol production process including a feedstock, a hammer mill, a slurry tank, a jet cooker, a liquefaction, a mash cooker, a fermentation, a beer, a distillation system, a whole stillage, a centrifuge, a thin stillage, an evaporator, a condensate, a syrup, a wet grain, a drum dryer, a distillers dried grain, distillers solubles, distillers wet grain, condensed distillers solubles distillers dried grains with solubles a molecular sieves, or any combination thereof. The alcohol production process can be in an ethanol production plant; a spirit or a drinkable alcohol production plant; or a fuel ethanol plant.

    摘要翻译： 公开了一种在酒精生产过程中使用热稳定性植酸酶来消除或减少植酸或植酸盐的方法。 植酸酶可以在醇生产过程的任何地方添加，包括原料，锤磨机，浆料罐，喷射炊具，液化，捣碎炉，发酵，啤酒，蒸馏系统，整个釜馏塔，离心机 蒸馏器，冷凝水，糖浆，湿颗粒，滚筒式干燥机，蒸馏器干燥谷物，酒糟可溶物，酒糟湿颗粒，浓缩酒糟可溶性酒精干燥谷物，可溶于分子筛或其任何组合 。 酒精生产过程可以在乙醇生产车间; 精神或饮用酒精生产厂; 或燃料乙醇厂。

    Phytases, nucleic acids encoding them and methods for making and using them
    2.
    发明授权
    Phytases, nucleic acids encoding them and methods for making and using them 有权
    植酸酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US08877478B2

    公开(公告)日：2014-11-04

    申请号：US12442207

    申请日：2007-09-21

    申请人： Brian Steer ,  Alberto Alvarado ,  Mark Dycaico ,  Fatima El-Farrah ,  Gerhard Frey ,  Katie A. Kline ,  Arne Solbak ,  Tomas Todaro ,  Axel Trefzer

    发明人： Brian Steer ,  Alberto Alvarado ,  Mark Dycaico ,  Fatima El-Farrah ,  Gerhard Frey ,  Katie A. Kline ,  Arne Solbak ,  Tomas Todaro ,  Axel Trefzer

    IPC分类号： C12N9/16 ,  C12N11/00 ,  C12P21/06 ,  C12P7/02 ,  C11C1/00 ,  C12P1/00 ,  A23L1/10 ,  A23C9/12 ,  A61K38/46 ,  C12P7/10

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23L33/18 ,  A23V2002/00 ,  C12P7/10 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072 ,  Y02E50/16 ,  Y02E50/17

    摘要： This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

    摘要翻译： 本发明涉及植酸酶，编码它们的多核苷酸，本发明的多核苷酸和多肽的用途，以及这些多核苷酸和多肽的产生和分离。 特别地，本发明提供了在高温条件下具有植酸酶活性的多肽，以及暴露于高温后保留活性的植酸酶。 除了较高的温度之外，本发明的植酸酶还可以在低温下耐热和/或耐热。 本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以配制成食物或饲料或补充剂，用于例如帮助消化植酸盐。 本发明的食品或饲料可以是丸粒，液体，粉末等的形式。 在一个方面，本发明的植酸酶在造粒期间对于热变性是稳定的; 并且这降低了植酸酶产品的成本，同时保持了体内功效并检测了饲料中的活性。

    PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    4.
    发明申请
    PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
    PHYTASES，NUCLEIC ACID ENCODING THEM AND METHODS FOR MAKING AND USING THEM

    公开(公告)号：US20150132383A1

    公开(公告)日：2015-05-14

    申请号：US14504849

    申请日：2014-10-02

    申请人： BASF ENZYMES, LLC

    发明人： Arne SOLBAK ,  Brian STEER ,  Mark DYCAICO ,  Katie A. KLINE ,  Axel TREFZER ,  Thomas TODARO ,  Fatima EL-FARRAH ,  Alberto ALVARADO ,  Gerhard FREY

    IPC分类号： C12N9/16 ,  A23K1/165 ,  A23L1/305

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23L33/18 ,  A23V2002/00 ,  C12P7/10 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072 ,  Y02E50/16 ,  Y02E50/17

    摘要： This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

    摘要翻译： 本发明涉及植酸酶，编码它们的多核苷酸，本发明的多核苷酸和多肽的用途，以及这些多核苷酸和多肽的产生和分离。 特别地，本发明提供了在高温条件下具有植酸酶活性的多肽，以及暴露于高温后保留活性的植酸酶。 除了较高的温度之外，本发明的植酸酶还可以在低温下耐热和/或耐热。 本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以配制成食物或饲料或补充剂，用于例如帮助消化植酸盐。 本发明的食品或饲料可以是丸粒，液体，粉末等的形式。 在一个方面，本发明的植酸酶在造粒期间对于热变性是稳定的; 并且这降低了植酸酶产品的成本，同时保持了体内功效并检测了饲料中的活性。

    METHODS FOR USING A THERMOSTABLE PHYTASE IN ETHANOL PRODUCTION
    5.
    发明申请
    METHODS FOR USING A THERMOSTABLE PHYTASE IN ETHANOL PRODUCTION 有权
    在乙醇生产中使用热稳定性物质的方法

    公开(公告)号：US20140017750A1

    公开(公告)日：2014-01-16

    申请号：US14032578

    申请日：2013-09-20

    申请人： VERENIUM CORPORATION

    发明人： Arne SOLBAK ,  Brian STEER ,  Mark DYCAICO ,  Katie A. KLINE ,  Axel TREFZER ,  Thomas TODARO ,  Fatima EL-FARRAH ,  Alberto ALVARADO ,  Gerhard FREY

    IPC分类号： C12P7/10

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23L33/18 ,  A23V2002/00 ,  C12P7/10 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072 ,  Y02E50/16 ,  Y02E50/17

    摘要： A method for using a thermostable phytase for eliminating or reducing phytic acid or salts of phytic acid in an alcohol production process is disclosed. The phytase can be added anywhere in the alcohol production process including a feedstock, a hammer mill, a slurry tank, a jet cooker, a liquefaction, a mash cooker, a fermentation, a beer, a distillation system, a whole stillage, a centrifuge, a thin stillage, an evaporator, a condensate, a syrup, a wet grain, a drum dryer, a distillers dried grain, distillers solubles, distillers wet grain, condensed distillers solubles distillers dried grains with solubles a molecular sieves, or any combination thereof. The alcohol production process can be in an ethanol production plant; a spirit or a drinkable alcohol production plant; or a fuel ethanol plant.

    摘要翻译： 公开了一种在酒精生产过程中使用热稳定性植酸酶来消除或减少植酸或植酸盐的方法。 植酸酶可以在醇生产过程的任何地方添加，包括原料，锤磨机，浆料罐，喷射炊具，液化，捣碎炉，发酵，啤酒，蒸馏系统，整个釜馏塔，离心机 蒸馏器，冷凝水，糖浆，湿粒子，滚筒式干燥机，蒸馏器干燥谷物，酒糟可溶物，酒糟湿颗粒，浓缩酒糟可溶性酒精干燥谷物，可溶于分子筛或其任何组合 。 酒精生产过程可以在乙醇生产车间; 精神或饮用酒精生产厂; 或燃料乙醇厂。

    THERMOSTABLE PHYTASE VARIANTS
    7.
    发明申请
    THERMOSTABLE PHYTASE VARIANTS 审中-公开
    热稳定的植物变种

    公开(公告)号：US20150159149A1

    公开(公告)日：2015-06-11

    申请号：US14627584

    申请日：2015-02-20

    申请人： NOVOZYMES A/S

    发明人： Lars Kobberoee Skov ,  Leonardo De Maria ,  Tomoko Matsui

    IPC分类号： C12N9/96 ,  A23K1/00 ,  C12P7/14 ,  A23L1/305

    CPC分类号： C12N9/96 ,  A23K10/16 ,  A23L33/195 ,  A61K38/46 ,  C12N9/16 ,  C12N15/8243 ,  C12P7/00 ,  C12P7/14 ,  C12P21/00 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072

    摘要： The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及一种生产植酸酶变体的方法，所述植酸酶变体与来自柠檬酸杆菌（Citrobacter braakii）的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少两个另外的二硫键。 这些植酸酶变体具有修饰的，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及产生的变体，编码这些植酸酶的DNA，其生产方法，以及其用途，例如在动物饲料和动物饲料添加剂中。

    Themostable Phytase Variants
    8.
    发明申请
    Themostable Phytase Variants 审中-公开
    稳定的植酸酶变体

    公开(公告)号：US20130195829A1

    公开(公告)日：2013-08-01

    申请号：US13636197

    申请日：2011-03-25

    申请人： Lars Koberoee Skov ,  Leonardo De Maria ,  Tomoko Matsui

    发明人： Lars Koberoee Skov ,  Leonardo De Maria ,  Tomoko Matsui

    IPC分类号： C12N9/96 ,  C12P7/14 ,  C12P21/00

    CPC分类号： C12N9/96 ,  A23K10/16 ,  A23L33/195 ,  A61K38/46 ,  C12N9/16 ,  C12N15/8243 ,  C12P7/00 ,  C12P7/14 ,  C12P21/00 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072

    摘要： The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.

    摘要翻译： 本发明涉及一种生产植酸酶变体的方法，所述植酸酶变体与来自柠檬酸杆菌（Citrobacter braakii）的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少两个另外的二硫键。 这些植酸酶变体具有修饰的，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及所生产的变体，编码这些植酸酶的DNA，其生产方法及其用途，例如， 在动物饲料和动物饲料添加剂中。