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    CELLULASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    3.
    发明申请
    CELLULASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
    细胞核，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US20130330783A1

    公开(公告)日：2013-12-12

    申请号：US13866790

    申请日：2013-04-19

    申请人： David Blum ,  Joslin Gemsch Cuenca ,  Mark Dycaico

    发明人： David Blum ,  Joslin Gemsch Cuenca ,  Mark Dycaico

    IPC分类号： C12P7/10 ,  C12N9/42

    CPC分类号： C12N9/2437 ,  C10L1/02 ,  C10L1/023 ,  C10L2200/0423 ,  C10L2200/0469 ,  C10L2290/26 ,  C12P7/10 ,  C12Y302/01004 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

    摘要： This invention relates to molecular and cellular biology and biochemistry. In one aspect, the invention provides polypeptides having cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or β-glucosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or β-glucosidase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts.

    摘要翻译： 本发明涉及分子和细胞生物学和生物化学。 一方面，本发明提供了具有纤维素酶活性的多肽，例如内切葡聚糖酶，纤维二糖水解酶，甘露聚糖酶和/或β-葡糖苷酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及多肽纤维素酶活性，例如内切葡聚糖酶，纤维二糖水解酶，甘露聚糖酶和/或β-葡糖苷酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 本发明的多肽可用于各种药物，农业，食品和饲料加工以及工业环境中。

    Phytases, nucleic acids encoding them and methods for making and using them
    5.
    发明授权
    Phytases, nucleic acids encoding them and methods for making and using them 有权
    植酸酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US08877478B2

    公开(公告)日：2014-11-04

    申请号：US12442207

    申请日：2007-09-21

    申请人： Brian Steer ,  Alberto Alvarado ,  Mark Dycaico ,  Fatima El-Farrah ,  Gerhard Frey ,  Katie A. Kline ,  Arne Solbak ,  Tomas Todaro ,  Axel Trefzer

    发明人： Brian Steer ,  Alberto Alvarado ,  Mark Dycaico ,  Fatima El-Farrah ,  Gerhard Frey ,  Katie A. Kline ,  Arne Solbak ,  Tomas Todaro ,  Axel Trefzer

    IPC分类号： C12N9/16 ,  C12N11/00 ,  C12P21/06 ,  C12P7/02 ,  C11C1/00 ,  C12P1/00 ,  A23L1/10 ,  A23C9/12 ,  A61K38/46 ,  C12P7/10

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23L33/18 ,  A23V2002/00 ,  C12P7/10 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072 ,  Y02E50/16 ,  Y02E50/17

    摘要： This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

    摘要翻译： 本发明涉及植酸酶，编码它们的多核苷酸，本发明的多核苷酸和多肽的用途，以及这些多核苷酸和多肽的产生和分离。 特别地，本发明提供了在高温条件下具有植酸酶活性的多肽，以及暴露于高温后保留活性的植酸酶。 除了较高的温度之外，本发明的植酸酶还可以在低温下耐热和/或耐热。 本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以配制成食物或饲料或补充剂，用于例如帮助消化植酸盐。 本发明的食品或饲料可以是丸粒，液体，粉末等的形式。 在一个方面，本发明的植酸酶在造粒期间对于热变性是稳定的; 并且这降低了植酸酶产品的成本，同时保持了体内功效并检测了饲料中的活性。

    Cellulases, nucleic acids encoding them and methods for making and using them
    6.
    发明授权
    Cellulases, nucleic acids encoding them and methods for making and using them 有权
    纤维素酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US08426184B2

    公开(公告)日：2013-04-23

    申请号：US11908672

    申请日：2006-01-13

    申请人： David Blum ,  Joslin Gemsch Cuenca ,  Mark Dycaico

    发明人： David Blum ,  Joslin Gemsch Cuenca ,  Mark Dycaico

    IPC分类号： C12N9/42 ,  C12N9/14 ,  C12N15/00 ,  C12N1/20 ,  C12P21/06 ,  C12P19/00 ,  C12P7/06 ,  A62D3/00 ,  C07H21/04 ,  A61K38/47 ,  A23C17/00 ,  A01H5/00

    CPC分类号： C12N9/2437 ,  C10L1/02 ,  C10L1/023 ,  C10L2200/0423 ,  C10L2200/0469 ,  C10L2290/26 ,  C12P7/10 ,  C12Y302/01004 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

    摘要： This invention relates to molecular and cellular biology and biochemistry. In one aspect, the invention provides polypeptides having cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or β-glucosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or β-glucosidase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts.

    摘要翻译： 本发明涉及分子和细胞生物学和生物化学。 一方面，本发明提供了具有纤维素酶活性的多肽，例如内切葡聚糖酶，纤维二糖水解酶，甘露聚糖酶和/或β-葡糖苷酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及多肽纤维素酶活性，例如内切葡聚糖酶，纤维二糖水解酶，甘露聚糖酶和/或β-葡糖苷酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 本发明的多肽可用于各种药物，农业，食品和饲料加工以及工业环境中。

    Proteases, nucleic acids encoding them and methods for making and using them
    8.
    发明授权
    Proteases, nucleic acids encoding them and methods for making and using them 有权
    蛋白酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US07642079B2

    公开(公告)日：2010-01-05

    申请号：US10530643

    申请日：2003-10-10

    申请人： Michelle Cayouette ,  Connie Jo Hansen ,  Amy McClure ,  May Sun ,  Svetlana Gramatikova ,  Mark Dycaico ,  Nelson R. Barton ,  Justin T. Stege ,  Nahla M. Aboushadi

    发明人： Michelle Cayouette ,  Connie Jo Hansen ,  Amy McClure ,  May Sun ,  Svetlana Gramatikova ,  Mark Dycaico ,  Nelson R. Barton ,  Justin T. Stege ,  Nahla M. Aboushadi

    IPC分类号： C12N9/48

    CPC分类号： C12N9/52 ,  A01K2217/05

    摘要： The invention is directed to polypeptides having protease activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of diagnostic, therapeutic, and industrial contexts. The polypeptides of the invention can be used as, e.g., an additive for a detergent, for processing foods and for chemical synthesis utilizing a reverse reaction. Additionally, the polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to ethanol diodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in increasing starch yield from corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.

    摘要翻译： 本发明涉及具有蛋白酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 本发明的多肽可用于多种诊断，治疗和工业背景。 本发明的多肽可用作例如用于洗涤剂的添加剂，用于加工食品和利用反向反应进行化学合成。 此外，本发明的多肽可用于食品加工，酿造，浴添加剂，醇生产，肽合成，对映选择性，皮革工业中的皮革制备，废物管理和动物降解，摄影工业中的银回收，医疗， 丝绸脱胶，生物膜降解，生物质转化为乙醇二聚体，抗微生物剂和消毒剂，个人护理和化妆品，生物技术试剂，用于增加玉米湿法研磨的淀粉产量，以及药物如消化助剂和抗炎（抗炎）剂。

    Proteases, nucleic acids encoding them and methods for making and using them
    9.
    发明申请
    Proteases, nucleic acids encoding them and methods for making and using them 有权
    蛋白酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US20060259995A1

    公开(公告)日：2006-11-16

    申请号：US10530643

    申请日：2003-10-10

    申请人： Michelle Cayouette ,  Connie Hansen ,  Amy McClure ,  May Sun ,  Svetlana Gramatikova ,  Mark Dycaico ,  Nelson Barton ,  Justin Stege ,  Nahla Aboushadi

    发明人： Michelle Cayouette ,  Connie Hansen ,  Amy McClure ,  May Sun ,  Svetlana Gramatikova ,  Mark Dycaico ,  Nelson Barton ,  Justin Stege ,  Nahla Aboushadi

    IPC分类号： A01K67/027 ,  C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C12N9/64 ,  A01H1/00

    CPC分类号： C12N9/52 ,  A01K2217/05

    摘要： The invention is directed to polypeptides having protease activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of diagnostic, therapeutic, and industrial contexts. The polypeptides of the invention can be used as, e.g., an additive for a detergent, for processing foods and for chemical synthesis utilizing a reverse reaction. Additionally, the polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to ethanol diodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in increasing starch yield from corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.

    摘要翻译： 本发明涉及具有蛋白酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 本发明的多肽可用于多种诊断，治疗和工业背景。 本发明的多肽可用作例如用于洗涤剂的添加剂，用于加工食品和利用反向反应进行化学合成。 此外，本发明的多肽可用于食品加工，酿造，浴添加剂，醇生产，肽合成，对映选择性，皮革工业中的皮革制备，废物管理和动物降解，摄影工业中的银回收，医疗， 丝绸脱胶，生物膜降解，生物质转化为乙醇二聚体，抗微生物剂和消毒剂，个人护理和化妆品，生物技术试剂，用于增加玉米湿法研磨的淀粉产量，以及药物如消化助剂和抗炎（抗炎）剂。