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公开(公告)号：US20080188375A1
公开(公告)日：2008-08-07
申请号：US11926120
申请日：2007-10-28
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07432048B2
公开(公告)日：2008-10-07
申请号：US09732622
申请日：2000-12-08
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q2525/301 , C12Q2561/109 , C12Q2565/501
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US07820387B2
公开(公告)日：2010-10-26
申请号：US11926120
申请日：2007-10-28
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07527928B2
公开(公告)日：2009-05-05
申请号：US10309584
申请日：2002-12-04
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/683
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US06692917B2
公开(公告)日：2004-02-17
申请号：US09940244
申请日：2001-08-27
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q168
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q2525/301 , C12Q2561/109 , C12Q2565/501
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on dendrimers and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在树枝状大分子上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07824862B2
公开(公告)日：2010-11-02
申请号：US12242508
申请日：2008-09-30
申请人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
发明人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
CPC分类号： C12Q1/6832 , C12Q1/701 , C12Q2565/133 , C12Q2521/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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7.发明授权Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides 失效通过结构桥接寡核苷酸的核酸结构探测进行多态性分析公开(公告)号：US06709819B2
公开(公告)日：2004-03-23
申请号：US09825574
申请日：2001-04-03
申请人： Victor I. Lyamichev , Fang Dong , Mary Ann D. Brow , Lance Fors , Bruce P. Neri
发明人： Victor I. Lyamichev , Fang Dong , Mary Ann D. Brow , Lance Fors , Bruce P. Neri
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2565/133
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US06709815B1
公开(公告)日：2004-03-23
申请号：US09402618
申请日：2000-07-18
申请人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
发明人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
IPC分类号： C12Q168
CPC分类号： C07H21/04 , C07H21/02 , C12Q1/68 , C12Q1/6827 , Y02A90/26 , C12Q2565/131 , C12Q2565/501 , C12Q2565/625 , C12Q2565/133
摘要： The present invention relates to methods and compns. for treating nucleic acids, and in particular, methods and compns. for the detection and characterization of nucleic acid sequences and sequence changes. The invention provides methods for examg. the conformations assumed by single strands of nucleic acid, forming the basis of novel methods of detection of specific nucleic acid sequences. The present invention contemplates use of novel detection methods for, among other uses, clinical diagnostic purposes, including but not limited to the detection and identification of pathogenic organisms. Examples are presented for the analysis of Mycobacterium tuberculosis and hepatitis C virus genes.
摘要翻译： 本发明涉及方法和方法。 用于处理核酸，特别是方法和化合物。 用于检测和表征核酸序列和序列变化。 本发明提供了检查方法。 由单链核酸假设的构象，形成检测特异性核酸序列的新方法的基础。 本发明考虑使用新的检测方法用于临床诊断目的，包括但不限于病原体的检测和鉴定。 介绍了分析结核分枝杆菌和丙型肝炎病毒基因的实例。
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公开(公告)号：US07429455B2
公开(公告)日：2008-09-30
申请号：US11515854
申请日：2006-09-05
申请人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
发明人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
CPC分类号： C12Q1/6832 , C12Q1/701 , C12Q2565/133 , C12Q2521/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US06759226B1
公开(公告)日：2004-07-06
申请号：US09577304
申请日：2000-05-24
申请人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
发明人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
IPC分类号： C12N922
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.
摘要翻译： 本发明涉及设计用于直接检测，表征和定量核酸，特别是RNA的新型酶。 本发明提供了识别在靶RNA序列上形成的特异性核酸切割结构并以位点特异性方式切割核酸切割结构以产生非靶切割产物的酶。 本发明提供具有改进的能够特异性切割包含DNA和RNA核酸链的复合物的DNA成员的酶的酶。
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