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公开(公告)号：US20080188375A1
公开(公告)日：2008-08-07
申请号：US11926120
申请日：2007-10-28
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07820387B2
公开(公告)日：2010-10-26
申请号：US11926120
申请日：2007-10-28
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07527928B2
公开(公告)日：2009-05-05
申请号：US10309584
申请日：2002-12-04
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/683
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US06692917B2
公开(公告)日：2004-02-17
申请号：US09940244
申请日：2001-08-27
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q168
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q2525/301 , C12Q2561/109 , C12Q2565/501
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on dendrimers and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在树枝状大分子上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07432048B2
公开(公告)日：2008-10-07
申请号：US09732622
申请日：2000-12-08
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q2525/301 , C12Q2561/109 , C12Q2565/501
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US07303869B2
公开(公告)日：2007-12-04
申请号：US10269222
申请日：2002-10-11
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
CPC分类号： C12Q1/683 , C12Q2561/109 , C12Q2565/101
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。 本发明涉及将核酸连接到固体支持物和修饰核酸的方法。 例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。
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公开(公告)号：US07884324B2
公开(公告)日：2011-02-08
申请号：US12130103
申请日：2008-05-30
申请人： Robert H. Blick , Michael S. Westphall , Hua Qin , Lloyd M. Smith
发明人： Robert H. Blick , Michael S. Westphall , Hua Qin , Lloyd M. Smith
CPC分类号： H01J1/32 , H01J29/023 , H01J43/246 , Y10S977/762
摘要： The present invention provides systems, devices, device components and structures for modulating the intensity and/or energies of electrons, including a beam of incident electrons. In some embodiments, for example, the present invention provides nano-structured semiconductor membrane structures capable of generating secondary electron emission. Nano-structured semiconductor membranes of this aspect of the present invention include membranes having an array of nanopillar structures capable of providing electron emission for amplification, filtering and/or detection of incident radiation, for example secondary electron emission and/or field emission. Nano-structured semiconductor membranes of the present invention are useful as converters wherein interaction of incident primary electrons and nanopillars of the nanopillar array generates secondary emission. Nano-structured semiconductor membranes of this aspect of the present invention are also useful as directed charge amplifiers wherein secondary emission from a nanopillar array provides gain functionality for increasing the intensity of radiation comprising incident electrons.
摘要翻译： 本发明提供了用于调制包括入射电子束的电子的强度和/或能量的系统，装置，器件组件和结构。 在一些实施例中，例如，本发明提供能够产生二次电子发射的纳米结构的半导体膜结构。 本发明该方面的纳米结构半导体膜包括具有能够提供用于放射，滤波和/或检测入射辐射的电子发射的纳米柱结构阵列的膜，例如二次电子发射和/或场发射。 本发明的纳米结构半导体膜可用作其中纳米柱阵列的入射一次电子和纳米柱的相互作用产生二次发射的转换器。 本发明该方面的纳米结构半导体膜也可用作定向电荷放大器，其中来自纳米柱阵列的二次发射提供了用于增加包括入射电子的辐射强度的增益功能。
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公开(公告)号：US5228971A
公开(公告)日：1993-07-20
申请号：US878632
申请日：1992-05-04
IPC分类号： G01N27/447
CPC分类号： G01N27/44704 , G01N27/44756
摘要： A horizontal electrophoresis assembly is described which is designed to utilize very thin gels of polyacrylamide. The assembly allows for water cooling of the gel during electrophoresis so that higher voltages can be applied to the gel without polymer degradation. The gel mold is made from a set of glass plates which are clamped into place defining a gel mold between them. In one embodiment, the site of field introduction is separated from the site of sample introduction so that the electric field is generally linear at the region of sample introduction.
摘要翻译： 描述了一种设计用于使用非常薄的聚丙烯酰胺凝胶的水平电泳组件。 该组件允许在电泳期间水冷却凝胶，使得更高的电压可以施加到凝胶上而没有聚合物降解。 凝胶模具由一组玻璃板制成，这些玻璃板被夹紧就位，在它们之间限定凝胶模具。 在一个实施例中，场引入位置与样品引入位置分离，使得电场在样品引入区域通常是线性的。
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公开(公告)号：US5171534A
公开(公告)日：1992-12-15
申请号：US558312
申请日：1990-10-15
IPC分类号： C12Q1/68 , G01N27/447
CPC分类号： G01N27/44726 , C12Q1/6869 , G01N27/44721 , Y10T436/143333
摘要： A system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations comprising: a source of chromophore or fluorescent tagged DNA fragments; a zone for contacting an electrophoresis gel; means for introducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.
摘要翻译： 用于在DNA测序操作中产生的DNA片段的电泳分析系统，包括：发色团或荧光标记的DNA片段; 用于接触电泳凝胶的区域; 用于将所述标记的DNA片段引入所述区域的方法; 以及用于在所述标记的DNA片段移动通过所述凝胶时监测所述标记的DNA片段的光度装置
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公开(公告)号：US5162654A
公开(公告)日：1992-11-10
申请号：US650393
申请日：1991-02-01
IPC分类号： G01J3/12 , G01N21/64 , G01N27/447
CPC分类号： G01N21/645 , G01N27/44721 , G01J2003/1226 , G01N2021/6421 , G01N2021/6441
摘要： An apparatus is disclosed to optically determine which of four fluorophores is attached to a band of DNA molecules on an electrophoresis gel. The apparatus includes four separate band pass interference filters and four wedge prisms to create four discrete areas of light on a detector. Digital comparison of the relative intensity of light sensed in the four discrete areas determines the identity of the excited fluorophore.
摘要翻译： 公开了一种用于光学确定四个荧光团中的哪一个附着到电泳凝胶上的DNA分子带上的装置。 该装置包括四个单独的带通干涉滤光器和四个楔形棱镜，以在检测器上产生四个离散的光区域。 在四个离散区域中感测的光的相对强度的数字比较确定激发的荧光团的身份。
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