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公开(公告)号：US06759226B1
公开(公告)日：2004-07-06
申请号：US09577304
申请日：2000-05-24
申请人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
发明人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
IPC分类号： C12N922
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.
摘要翻译： 本发明涉及设计用于直接检测，表征和定量核酸，特别是RNA的新型酶。 本发明提供了识别在靶RNA序列上形成的特异性核酸切割结构并以位点特异性方式切割核酸切割结构以产生非靶切割产物的酶。 本发明提供具有改进的能够特异性切割包含DNA和RNA核酸链的复合物的DNA成员的酶的酶。
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公开(公告)号：US06635463B2
公开(公告)日：2003-10-21
申请号：US09758282
申请日：2001-01-11
申请人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
发明人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
IPC分类号： C12N922
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.
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公开(公告)号：US07807806B2
公开(公告)日：2010-10-05
申请号：US11929404
申请日：2007-10-30
申请人： Hatim T. Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael W. Kaiser , Robert W. Kwiatkowski , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , Wupo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim T. Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael W. Kaiser , Robert W. Kwiatkowski , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , Wupo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20090142754A1
公开(公告)日：2009-06-04
申请号：US11929404
申请日：2007-10-30
申请人： Hatim T. Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael W. Kaiser , Robert W. Kwiatkowski , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim T. Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael W. Kaiser , Robert W. Kwiatkowski , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07150982B2
公开(公告)日：2006-12-19
申请号：US10084839
申请日：2002-02-26
申请人： Hatim T. Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael W. Kaiser , Robert W. Kwiatkowski , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim T. Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael W. Kaiser , Robert W. Kwiatkowski , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
IPC分类号： C12N9/22
CPC分类号： C12N9/1252 , C12N9/22 , C12Q1/6809 , C12Q1/6823 , C12Q1/6827 , H04L29/06 , H04L29/06027 , H04L69/32 , C12Q2561/109 , C12Q2537/162 , C12Q2525/161
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US07935800B2
公开(公告)日：2011-05-03
申请号：US11642815
申请日：2006-12-19
申请人： Hatim T. Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael W. Kaiser , Robert W. Kwiatkowski , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim T. Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael W. Kaiser , Robert W. Kwiatkowski , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y Takova , Lisa C. Thompson , Kevin L. Vedvik
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20080293046A1
公开(公告)日：2008-11-27
申请号：US11642815
申请日：2006-12-19
申请人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, JR. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, JR. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20110104682A1
公开(公告)日：2011-05-05
申请号：US12898490
申请日：2010-10-05
申请人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, JR. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, JR. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US08063184B2
公开(公告)日：2011-11-22
申请号：US12898490
申请日：2010-10-05
申请人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, Jr. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, Jr. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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