公开(公告)号：US12258613B2

公开(公告)日：2025-03-25

申请号：US16492588

申请日：2018-03-08

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： James R. Heath ,  Songming Peng ,  Alphonsus Hon-Chung Ng

IPC: C12Q1/6804 ,  B01L3/00 ,  C12N15/10

Abstract: Compositions and methods for identifying antigen-specific T cells, including determining paired T cell receptor sequences for a specific antigen, are described. Compositions and methods for identifying neoantigen-specific T cells are also described. Microfluidic devices useful for identifying antigen-specific T cells, and methods of using the same, are also described.

公开(公告)号：US10481158B2

公开(公告)日：2019-11-19

申请号：US15170919

申请日：2016-06-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： James R. Heath ,  Songming Peng

IPC: G01N33/53 ,  C12Q1/68 ,  C07H21/04 ,  G01N33/569 ,  C12Q1/6804 ,  G01N15/14 ,  G01N33/574 ,  G01N33/68 ,  G01N15/00

Abstract: Compositions and methods for isolating patient-derived antigen-specific T cells include an antigen complex having a polynucleotide barcoded nanoparticle sorting agent complexed with a peptide-loaded streptavidin major histocompatability complex (MHC) tetramer, the barcoding technology allowing for high fidelity screening of a library of the antigen complexes to readily isolate and identify antigen-specific T cells.

公开(公告)号：US20170003288A1

公开(公告)日：2017-01-05

申请号：US15170919

申请日：2016-06-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： James R. Heath ,  Songming Peng

IPC: G01N33/569 ,  G01N15/14 ,  G01N33/574 ,  C12Q1/68 ,  G01N33/68

CPC classification number: G01N33/56972 ,  C07H21/04 ,  C12Q1/6804 ,  G01N15/14 ,  G01N33/574 ,  G01N33/6878 ,  G01N2015/008 ,  G01N2015/149 ,  G01N2333/70539 ,  G01N2458/10 ,  C12Q2563/131 ,  C12Q2563/143 ,  C12Q2563/155 ,  C12Q2565/514

Abstract: Compositions and methods for isolating patient-derived antigen-specific T cells include an antigen complex having a polynucleotide barcoded nanoparticle sorting agent complexed with a peptide-loaded streptavidin major histocompatability complex (MHC) tetramer, the barcoding technology allowing for high fidelity screening of a library of the antigen complexes to readily isolate and identify antigen-specific T cells.

Abstract translation: 用于分离患者衍生的抗原特异性T细胞的组合物和方法包括具有与加载肽的链霉抗生物素蛋白主要组织相容性复合物（MHC）四聚体复合的多核苷酸条形码纳米颗粒分选试剂的抗原复合物，该条形码技术允许文库的高保真度筛选 的抗原复合物以容易地分离和鉴定抗原特异性T细胞。

公开(公告)号：US11384327B2

公开(公告)日：2022-07-12

申请号：US16347559

申请日：2017-11-01

Applicant: California Institute of Technology ,  Gwangju Institute of Science and Technology (GIST)

Inventor： Songming Peng ,  James R. Heath ,  William Chour ,  Alphonsus Hon-Chung Ng ,  Sung Yang ,  Jongchan Choi ,  Ji-Chul Hyun

IPC: C12M3/06 ,  B01L3/00 ,  G01N33/569 ,  B01F25/433 ,  B01F33/30

Abstract: Disclosed herein are methods and devices for antigen-specific T cell identification or neoantigen identification. Also disclosed herein are devices for separating and isolating antigen-specific T cells or other particles of a certain size from a population of particles of different sizes. Also describe herein are methods and devices for the separation and isolation of barcoded T cells from other nanoparticles containing barcodes for subsequent analysis and further processing of a viable T cell.

公开(公告)号：US20200040293A1

公开(公告)日：2020-02-06

申请号：US16347559

申请日：2017-11-01

Applicant: California Institute of Technology ,  Gwangju Institute of Science and Technology (GIST)

Inventor： Songming Peng ,  James R. Heath ,  William Chour ,  Alphonsus H.c. Ng ,  Sung Yang ,  Jongchan Choi ,  Ji-Chul Hyun

IPC: C12M3/06 ,  B01F13/00 ,  B01F5/06 ,  B01L3/00 ,  G01N33/569

Abstract: Disclosed herein are methods and devices for antigen-specific T cell identification or neoantigen identification. Also disclosed herein are devices for separating and isolating antigen- specific T cells or other particles of a certain size from a population of particles of different sizes. Also describe herein are methods and devices for the separation and isolation of barcoded T cells from other nanoparticles containing barcodes for subsequent analysis and further processing of a viable T cell.

公开(公告)号：US20230069843A1

公开(公告)日：2023-03-09

申请号：US16492588

申请日：2018-03-08

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： James R. Heath ,  Songming Peng ,  Alphonsus Hon-Chung Ng

IPC: C12N15/10 ,  B01L3/00

Abstract: Compositions and methods for identifying antigen-specific T cells, including determining paired T cell receptor sequences for a specific antigen, are described. Compositions and methods for identifying neoantigen-specific T cells are also described. Microfluidic devices useful for identifying antigen-specific T cells, and methods of using the same, are also described.