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    APPARATUS AND METHOD FOR CELL DISRUPTION
    1.
    发明申请
    APPARATUS AND METHOD FOR CELL DISRUPTION 有权
    细胞破坏的装置和方法

    公开(公告)号：US20150352545A1

    公开(公告)日：2015-12-10

    申请号：US14737853

    申请日：2015-06-12

    Applicant: CEPHEID

    Inventor： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Stanley H. Sakai ,  Jesus Ching

    IPC: B01L3/00 ,  C12M1/00

    CPC classification number: B01L3/508 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/025 ,  B01L2300/0848 ,  B01L2300/12 ,  C12M47/06 ,  C12N1/066 ,  C12N13/00

    Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

    Abstract translation: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。

    METHOD FOR SEPARATING AN ANALYTE FROM A SAMPLE
    2.
    发明申请
    METHOD FOR SEPARATING AN ANALYTE FROM A SAMPLE 审中-公开
    从样品中分离分析物的方法

    公开(公告)号：US20140141424A1

    公开(公告)日：2014-05-22

    申请号：US14061448

    申请日：2013-10-23

    Applicant: Cepheid

    Inventor： FARZAD POURAHMADI ,  William McMillan ,  Jesus Ching ,  Ronald Chang ,  Lee A. Christel ,  Gregory T.A. Kovacs ,  M. Allen Northrup ,  Kurt E. Petersen

    IPC: C12N15/10

    CPC classification number: C12N15/1017 ,  B01D15/00 ,  B01L7/52 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M47/06 ,  G01N1/34 ,  Y10T436/255

    Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

    Abstract translation: 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室，其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径，所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室，从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径，将捕获的分析物从萃取室洗脱。

    COMPOSITION, APPARATUS, AND METHOD FOR SEPARATING AN ANALYTE FROM A SAMPLE
    3.
    发明申请
    COMPOSITION, APPARATUS, AND METHOD FOR SEPARATING AN ANALYTE FROM A SAMPLE 审中-公开
    用于从样品中分离分析物的组合物，装置和方法

    公开(公告)号：US20130236907A1

    公开(公告)日：2013-09-12

    申请号：US13862325

    申请日：2013-04-12

    Applicant: CEPHEID

    Inventor： Kurt E. PETERSEN ,  William A. McMillan ,  Lee A. Christel ,  Ronald Chang ,  Farzad Pourahmadi ,  Jesus Ching ,  Gregory T.A. Kovacs ,  M. Allen Northrup

    IPC: G01N1/34

    CPC classification number: C12N15/1017 ,  B01D15/00 ,  B01L7/52 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M47/06 ,  G01N1/34 ,  Y10T436/255

    Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

    Abstract translation: 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室，其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径，所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室，从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径，将捕获的分析物从萃取室洗脱。

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