公开(公告)号：US20050196783A1

公开(公告)日：2005-09-08

申请号：US11022546

申请日：2004-12-23

申请人： Corey Goodman ,  Thomas Kidd ,  Katja Brose ,  Marc Tessier-Lavigne

发明人： Corey Goodman ,  Thomas Kidd ,  Katja Brose ,  Marc Tessier-Lavigne

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/17 ,  A61P43/00 ,  C07K14/47 ,  C07K14/705 ,  C07K16/18 ,  C12N15/12 ,  G01N33/15 ,  G01N33/50 ,  G01N33/566 ,  G01N33/68 ,  C12Q1/68 ,  A61K39/395 ,  C07H21/04 ,  C07K16/28 ,  G01N33/53 ,  G01N33/567

CPC分类号： C07K14/70503 ,  A61K38/00 ,  C07K14/4702 ,  G01N33/566 ,  G01N33/68 ,  G01N2333/43573 ,  G01N2500/02

摘要： Disclosed are methods and compositions for identifying agents which modulate the interaction of Robo and a Robo ligand and for modulating the interaction of Robo and a Robo ligand. The methods for identifying Robo:ligand modulators find particular application in commercial drug screens. These methods generally comprise (1) combining a Robo polypeptide, a Slit polypeptide and a candidate agent under conditions whereby, but for the presence of the agent, the Robo and Slit polypeptides engage in a first interaction, and (2) determining a second interaction of the Robo and Slit polypeptides in the presence of the agent, wherein a difference between the first and second interactions indicates that the aget modulates the interaction of the Robo and Slit polypeptides. The subject methods of modulating the interaction of Robo and a Robo ligand involve combining a Robo polypeptide, a Slit polypeptide and a modulator under conditions whereby, but for the presence of the modulator, the Robo and Slit polypeptides engage in a first interaction, whereby the Robo and Slit polypeptides engage in a second interaction different from the first interaction. In a particular embodiment, the modulator is dominant negative form of the Robo or Slit polypeptide.

公开(公告)号：US20050059040A1

公开(公告)日：2005-03-17

申请号：US10826812

申请日：2004-04-16

申请人： Corey Goodman ,  Thomas Kidd ,  Kevin Mitchell ,  Guy Tear

发明人： Corey Goodman ,  Thomas Kidd ,  Kevin Mitchell ,  Guy Tear

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K39/395 ,  A61P25/00 ,  A61P43/00 ,  C07K14/475 ,  C07K14/48 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/18 ,  C12P21/02 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/705 ,  G01N33/53

CPC分类号： C07K14/475 ,  A61K38/00

摘要： Robo1 and Robo2 polypeptides may be produced recombinantly from transformed host cells from the disclosed Robo encoding nucleic acids or purified from human cells. The invention provided isolated Robo hybridization probes and primers capable of specifically hybridizing with the disclosed Robo genes, Robo-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

摘要翻译： Robo1和Robo2多肽可以从所公开的Robo编码核酸的转化的宿主细胞重组产生或从人细胞纯化产生。 本发明提供了能够与所公开的Robo基因，Robo特异性结合剂如特异性抗体特异性杂交的分离的Robo杂交探针和引物，以及在诊断，治疗和生物制药工业中制备和使用主题组合物的方法。

公开(公告)号：US20070033669A1

公开(公告)日：2007-02-08

申请号：US11580373

申请日：2006-10-12

申请人： Corey Goodman ,  Alex Kolodkin ,  David Matthes ,  David Bentley ,  Timothy O'Connor

发明人： Corey Goodman ,  Alex Kolodkin ,  David Matthes ,  David Bentley ,  Timothy O'Connor

IPC分类号： A01K67/027 ,  A61K38/10 ,  A61K38/08 ,  C07K14/47 ,  C12N5/06 ,  C07K16/18

CPC分类号： C12N15/8509 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2267/01 ,  A01K2267/03 ,  A01K2267/0318 ,  A61K38/00 ,  C07K14/005 ,  C07K14/4703 ,  C07K14/705 ,  C12N2710/24122

摘要： A novel class of proteins, semaphorins, nucleic acids encoding semaphorins, semaphorin peptides, and methods of using semaphorins and semaphorin-encoding nucleic acids are disclosed. Semaphorin peptides and receptor agonists and antagonists provide potent modulators of nerve cell growth and regeneration. The invention provides pharmaceutical compositions, methods for screening chemical libraries for regulators of cell growth/differentiation; semaphorin gene-derived nucleic acids for use in genetic mapping, as probes for related genes, and as diagnostic reagents for genetic neurological disease; specific cellular and animal systems for the development of neurological disease therapy.

摘要翻译： 公开了一类新颖的蛋白质，信号素，编码信号素的核酸，信号素肽，以及使用信号素和信号素编码核酸的方法。 信号肽和受体激动剂和拮抗剂提供神经细胞生长和再生的有效调节剂。 本发明提供药物组合物，用于筛选细胞生长/分化调节物的化学文库的方法; 用于遗传作图的信号素基因衍生的核酸，作为相关基因的探针，以及作为遗传神经疾病的诊断试剂; 特异性细胞和动物系统用于神经疾病治疗的发展。