公开(公告)号：US20150329843A1

公开(公告)日：2015-11-19

申请号：US14793786

申请日：2015-07-08

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter LOS ,  Cornelis Maria Jacobus SAGT ,  Margot Elisabeth Francoise SCHOONNEVELD-BERGMANS ,  Robbertus Antonius DAMVELD

IPC: C12N9/42 ,  C12P19/02 ,  C12P19/14

CPC classification number: C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12N9/2477 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/343

Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamentous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be used in a process for the saccharification of cellulosic material.

Abstract translation: 本发明涉及一种宿主细胞，其包含至少四种不同的异源多核苷酸，其选自编码纤维素酶，半纤维素酶和果胶酶的多核苷酸，其中所述宿主细胞能够产生选自纤维素酶，半纤维素酶和 果胶酶，其中所述宿主细胞是丝状真菌，并且能够分泌所述至少四种不同的酶。 该宿主细胞适合用于生产可用于纤维素材料糖化过程的酶组合物。

公开(公告)号：US20170114337A1

公开(公告)日：2017-04-27

申请号：US15386967

申请日：2016-12-21

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter LOS ,  Cornelis Maria Jacobus SAGT ,  Margot Elisabeth Francoise SCHOONNEVELD-BERGMANS ,  Robbertus Antonius DAMVELD

IPC: C12N9/42 ,  C12P19/02 ,  C12P19/14

CPC classification number: C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12N9/2477 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/343

Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamen-tous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be used in a process for the saccharification of cellulosic material.

公开(公告)号：US20140031587A1

公开(公告)日：2014-01-30

申请号：US14044722

申请日：2013-10-02

Applicant: DSM IP Assets B.V.

Inventor： Rene VERWAAL ,  Liang WU ,  Robbertus Antonius DAMVELD ,  Cornelis Maria Jacobus SAGT

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C12N9/001 ,  C12N9/88 ,  C12N15/81 ,  C12N15/815 ,  Y02P20/52

Abstract: The present invention relates to a recombinant eukaryotic cell selected from a yeast of a filamentous fungus comprising a nucleotide sequence encoding a NAD(H)-dependent fumarate reductase that catalyses the conversion of fumaric acid to succinic acid. The invention further relates to a process for the production of succinic acid wherein the eukaryotic cell according to the present invention is used.

Abstract translation: 本发明涉及选自丝状真菌酵母的重组真核细胞，其包含编码NAD（H） - 依赖性富马酸酯还原酶的核苷酸序列，其催化富马酸转化为琥珀酸。 本发明还涉及生产琥珀酸的方法，其中使用本发明的真核细胞。

公开(公告)号：US20210087544A1

公开(公告)日：2021-03-25

申请号：US17108622

申请日：2020-12-01

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter LOS ,  Cornelis Maria Jacobus SAGT ,  Margot Elisabeth Francoise SCHOONEVELD-BERGMANS ,  Robbertus Antonius DAMVELD

IPC: C12N9/42 ,  C12P19/02 ,  C12P19/14 ,  C12N9/24

Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chose from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filam-tous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be use in a process for the saccharification of cellulosic material.

公开(公告)号：US20140065680A1

公开(公告)日：2014-03-06

申请号：US14070770

申请日：2013-11-04

Applicant: DSM IP ASSETS B.V.

Inventor： Rene VERWAAL ,  Liang WU ,  Robbertus Antonius DAMVELD ,  Cornelis Maria Jacobus SAGT

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C12N9/88 ,  Y02P20/52

Abstract: The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.

Abstract translation: 本发明涉及包含编码异源酶的核苷酸序列的重组酵母，其催化苹果酸向富马酸的转化。 本发明还涉及一种生产二羧酸的方法，其中使用了本发明的酵母。

公开(公告)号：US20140045230A1

公开(公告)日：2014-02-13

申请号：US14059284

申请日：2013-10-21

Applicant: DSM IP ASSETS B.V.

Inventor： Rene VERWAAL ,  Liang WU ,  Robbertus Antonius DAMVELD ,  Cornelis Maria Jacobus SAGT

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C12N9/88

Abstract: The present invention relates to a recombinant fungus comprising an enzyme which catalysts the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used.

Abstract translation: 本发明涉及一种重组真菌，其包含催化苹果酸在胞质溶胶中转化为富马酸的酶。 本发明还涉及生产二羧酸如富马酸和琥珀酸的方法，其中使用重组真菌。