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公开(公告)号：US20230049760A1
公开(公告)日：2023-02-16
申请号：US17789598
申请日：2020-12-18
Applicant: DSM IP ASSETS B.V.
Inventor： Jenna MCMAHON , Elvin Irsan KOOI , Liang WU , René Marcel DE JONG , Valmik Kanubhai VYAS , Peter Louis HOUSTON
Abstract: The present invention is related to a retinoid-producing host cell, particularly oleaginous yeast, modified such that the percentage of retinyl acetate based on the total retinoids produced by such host cell is increased during fermentation using triglyceride oils, like for example vegetable oil, as carbon source, wherein the activity of certain endogenous hydrolases or transferases involved in undesired conversions of retinol or retinol acetate is reduced or abolished. Particularly, such modified host cell might be useful in a biotechnological process for production of vitamin A.
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公开(公告)号：US20170191091A1
公开(公告)日：2017-07-06
申请号：US15314494
申请日：2015-05-28
Applicant: DSM IP Assets B.V.
Inventor： Zheng ZHAO , Bernard MEIJRINK , Robertus Antonius Mijndert VAN DER HOEVEN , Liang WU , Johannes Andries ROUBOS , Marina VAN GELDER
Abstract: The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid, 4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
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Patent Agency Ranking
公开(公告)号：US20170191089A1
公开(公告)日：2017-07-06
申请号：US15314492
申请日：2015-05-28
Applicant: DSM IP Assets B.V.
Inventor： Zheng ZHAO , Bernard MEIJRINK , Robertus Antonius Mijindert VAN DER HOEVEN , Liang WU , Johannes Andries ROUBOS
CPC classification number: C12P7/44 , C07K14/395 , C12N9/0008 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/62 , C12Y203/03001 , C12Y401/01006 , C12Y401/01031 , C12Y401/01032 , C12Y604/01001
Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
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公开(公告)号：US20140030730A1
公开(公告)日：2014-01-30
申请号：US14045683
申请日：2013-10-03
Applicant: DSM IP ASSETS B.V.
Inventor： Ulrike Maria MUELLER , Liang WU , Lourina Madeleine RAAMSDONK , Aaron Adriaan WINKLER
Abstract: The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.
Abstract translation: 本发明涉及一种鉴定具有用于将丙酮酸，乙醛或乙酸酯转化成酵母细胞的（细胞溶质）中的乙酰辅酶A的酶活性的异源多肽的方法，包括：a）提供包含至少一个缺失的突变的酵母细胞 选自编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和乙酰辅酶A合成酶（ACS）的基因的（PDH）旁路的一个基因; b）用包含异源核苷酸序列的表达载体转化所述突变的酵母细胞，所述异源核苷酸序列编码具有将丙酮酸，乙醛或乙酸转化为乙酰辅酶A的潜在酶活性的候选多肽; c）测试所述重组突变酵母细胞在含有葡萄糖作为唯一碳源的基本培养基上生长的能力，以及d）将所述候选多肽鉴定为异源多肽，其具有将丙酮酸，乙醛或乙酸酯转化成乙酰辅酶A 当所述细胞的生长被观察时，所述酵母细胞的胞质溶胶。 本发明还涉及生产发酵生产如丁醇的方法。
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公开(公告)号：US20140065680A1
公开(公告)日：2014-03-06
申请号：US14070770
申请日：2013-11-04
Applicant: DSM IP ASSETS B.V.
Inventor： Rene VERWAAL , Liang WU , Robbertus Antonius DAMVELD , Cornelis Maria Jacobus SAGT
IPC: C12P7/46
Abstract: The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.
Abstract translation: 本发明涉及包含编码异源酶的核苷酸序列的重组酵母，其催化苹果酸向富马酸的转化。 本发明还涉及一种生产二羧酸的方法，其中使用了本发明的酵母。
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公开(公告)号：US20140045230A1
公开(公告)日：2014-02-13
申请号：US14059284
申请日：2013-10-21
Applicant: DSM IP ASSETS B.V.
Inventor： Rene VERWAAL , Liang WU , Robbertus Antonius DAMVELD , Cornelis Maria Jacobus SAGT
IPC: C12P7/46
Abstract: The present invention relates to a recombinant fungus comprising an enzyme which catalysts the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used.
Abstract translation: 本发明涉及一种重组真菌，其包含催化苹果酸在胞质溶胶中转化为富马酸的酶。 本发明还涉及生产二羧酸如富马酸和琥珀酸的方法，其中使用重组真菌。
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公开(公告)号：US20140031587A1
公开(公告)日：2014-01-30
申请号：US14044722
申请日：2013-10-02
Applicant: DSM IP Assets B.V.
Inventor： Rene VERWAAL , Liang WU , Robbertus Antonius DAMVELD , Cornelis Maria Jacobus SAGT
IPC: C12P7/46
Abstract: The present invention relates to a recombinant eukaryotic cell selected from a yeast of a filamentous fungus comprising a nucleotide sequence encoding a NAD(H)-dependent fumarate reductase that catalyses the conversion of fumaric acid to succinic acid. The invention further relates to a process for the production of succinic acid wherein the eukaryotic cell according to the present invention is used.
Abstract translation: 本发明涉及选自丝状真菌酵母的重组真核细胞，其包含编码NAD（H） - 依赖性富马酸酯还原酶的核苷酸序列，其催化富马酸转化为琥珀酸。 本发明还涉及生产琥珀酸的方法，其中使用本发明的真核细胞。
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