公开(公告)号：US20150232891A1

公开(公告)日：2015-08-20

申请号：US14662594

申请日：2015-03-19

Applicant: DSM IP ASSETS B.V.

Inventor： Mickel Leonardus August JANSEN ,  Rene VERWAAL

IPC: C12P7/46 ,  C12P7/44

CPC classification number: C12P7/46 ,  C12P7/44

Abstract: The present invention relates to a process for the production of a dicarboxylic acid. The process comprises fermenting a yeast in the presence of a carbohydrate-containing substrate and low amounts of oxygen at a pH value at which at least 50% of the dicarboxylic acid is in the acid form. The process of the present invention allows for high yields of the dicarboxylic acid product and is more cost-effective than existing processes in which the salt is produced which during recovery has to be converted to the acid. It also leads to a simpler and more convenient downstream processing.

Abstract translation: 本发明涉及二羧酸的制备方法。 该方法包括在含有碳水化合物的底物和少量氧的存在下，在至少50％的二羧酸是酸形式的pH值下发酵酵母。 本发明的方法允许高产率的二羧酸产物，并且比生产盐的现有方法更具成本效益，在回收期间必须将其转化为酸。 它也可以实现更简单和更方便的下游处理。

公开(公告)号：US20210071174A1

公开(公告)日：2021-03-11

申请号：US17053265

申请日：2019-05-06

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries ROUBOS ,  Rene VERWAAL ,  Brenda VONK

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/81

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR transient expression construct (CTEC) for a genome editing system.

公开(公告)号：US20240263172A1

公开(公告)日：2024-08-08

申请号：US18594869

申请日：2024-03-04

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries ROUBOS ,  Rene VERWAAL ,  Brenda VONK

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/81

CPC classification number: C12N15/11 ,  C12N9/22 ,  C12N15/81 ,  C12N2310/20 ,  C12N2330/51

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR transient expression construct (CTEC) for a genome editing system.

公开(公告)号：US20140031587A1

公开(公告)日：2014-01-30

申请号：US14044722

申请日：2013-10-02

Applicant: DSM IP Assets B.V.

Inventor： Rene VERWAAL ,  Liang WU ,  Robbertus Antonius DAMVELD ,  Cornelis Maria Jacobus SAGT

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C12N9/001 ,  C12N9/88 ,  C12N15/81 ,  C12N15/815 ,  Y02P20/52

Abstract: The present invention relates to a recombinant eukaryotic cell selected from a yeast of a filamentous fungus comprising a nucleotide sequence encoding a NAD(H)-dependent fumarate reductase that catalyses the conversion of fumaric acid to succinic acid. The invention further relates to a process for the production of succinic acid wherein the eukaryotic cell according to the present invention is used.

Abstract translation: 本发明涉及选自丝状真菌酵母的重组真核细胞，其包含编码NAD（H） - 依赖性富马酸酯还原酶的核苷酸序列，其催化富马酸转化为琥珀酸。 本发明还涉及生产琥珀酸的方法，其中使用本发明的真核细胞。

公开(公告)号：US20220380759A1

公开(公告)日：2022-12-01

申请号：US17817899

申请日：2022-08-05

Applicant: DSM IP ASSETS B.V.

Inventor： James Casey LIPPMEIER ,  Yelena BETZ ,  Johannes Andries ROUBOS ,  Rene VERWAAL

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/86 ,  C12P21/00

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-Cas system for a Labyrinthulomycetes host cell.

公开(公告)号：US20220056460A1

公开(公告)日：2022-02-24

申请号：US17299413

申请日：2019-11-29

Applicant: DSM IP ASSETS B.V.

Inventor： Rene VERWAAL ,  Nathalie WIESSENHAAN ,  Johannes Andries ROUBOS

IPC: C12N15/81 ,  C12N15/11 ,  C12N1/16 ,  C12N15/10

Abstract: The invention relates to the field of molecular biology and cell biology. More specifically, the invention relates to CRISPR guide-RNA expression strategies for multiplex genome engineering.

公开(公告)号：US20140065680A1

公开(公告)日：2014-03-06

申请号：US14070770

申请日：2013-11-04

Applicant: DSM IP ASSETS B.V.

Inventor： Rene VERWAAL ,  Liang WU ,  Robbertus Antonius DAMVELD ,  Cornelis Maria Jacobus SAGT

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C12N9/88 ,  Y02P20/52

Abstract: The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.

Abstract translation: 本发明涉及包含编码异源酶的核苷酸序列的重组酵母，其催化苹果酸向富马酸的转化。 本发明还涉及一种生产二羧酸的方法，其中使用了本发明的酵母。

公开(公告)号：US20140045230A1

公开(公告)日：2014-02-13

申请号：US14059284

申请日：2013-10-21

Applicant: DSM IP ASSETS B.V.

Inventor： Rene VERWAAL ,  Liang WU ,  Robbertus Antonius DAMVELD ,  Cornelis Maria Jacobus SAGT

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C12N9/88

Abstract: The present invention relates to a recombinant fungus comprising an enzyme which catalysts the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used.

Abstract translation: 本发明涉及一种重组真菌，其包含催化苹果酸在胞质溶胶中转化为富马酸的酶。 本发明还涉及生产二羧酸如富马酸和琥珀酸的方法，其中使用重组真菌。