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    Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
    1.
    发明授权
    Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers 有权
    使用多官能团涂覆的固相载体分离核酸的方法

    公开(公告)号：US07527929B2

    公开(公告)日：2009-05-05

    申请号：US11194072

    申请日：2005-07-29

    Applicant: Kevin J. McKernan ,  Erik Gustafson ,  Adrianne D. Brand

    Inventor： Kevin J. McKernan ,  Erik Gustafson ,  Adrianne D. Brand

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q2527/125

    Abstract: The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e.g., buffer) that selectively removes (e.g., elutes) either the nucleic acid from the first functional group or the target nucleic acid species from the second functional group of the solid phase carriers, thereby isolating a target nucleic acid species from a mixture comprising a plurality of nucleic acid species.

    Abstract translation: 本发明涉及从混合物中分离靶物种（例如靶核酸物种）的方法。 在本发明的方法中，将混合物与具有包含多个官能团的表面的固相载体组合，其中一个可逆地和选择性地结合靶物种。 在一个具体实施方案中，将混合物与具有可逆结合核酸的第一官能团的固相载体和选择性和可逆结合靶核酸物质的第二官能团组合，从而产生第一组合。 第一组合在适于将核酸与第一官能团结合并且靶核酸物质与第二官能团结合的条件下保持。 将固相载体与第一组合分离，并与选择性地除去（例如，洗脱）来自第一官能团的核酸或来自第二官能团的第二官能团的靶核酸物质的试剂（例如，缓冲液）组合 固相载体，从而从包含多个核酸物质的混合物中分离靶核酸物质。

    METHODS AND SYSEMS FOR PERFORMING REAL-TIME RECOMMENDATION PROCESSING
    2.
    发明申请
    METHODS AND SYSEMS FOR PERFORMING REAL-TIME RECOMMENDATION PROCESSING 审中-公开
    执行实时建议处理的方法和步骤

    公开(公告)号：US20110246465A1

    公开(公告)日：2011-10-06

    申请号：US12987932

    申请日：2011-01-10

    Applicant: Jari Koister ,  Erik Gustafson

    Inventor： Jari Koister ,  Erik Gustafson

    IPC: G06F17/30

    CPC classification number: G06F16/3325 ,  G06F16/3347 ,  G06F16/353 ,  G06F16/355

    Abstract: Methods and systems are presented for recommending similar questions to one that a user has entered into a search engine. Previously-entered questions are subject to a clustering algorithm and placed into a hierarchy of clusters, with clusters set within clusters. For each cluster within the hierarchy, a representative vector, based on feature vectors of the items within the cluster, is calculated. A feature vector for the user's question is calculated and used, along with the representative vectors at each level in the hierarchy, to traverse and navigate the cluster hierarchy. When a leaf cluster is found, the items in the leaf cluster, such as the previously-entered questions are returned to the user. A subset of items in the leaf cluster, or items from other leaf clusters within a branch cluster, can be selected based on the number of items desired to be returned.

    Abstract translation: 提出了方法和系统，用于向用户输入搜索引擎的类似问题提出类似的问题。 先前输入的问题受聚类算法的影响，并置于群集的层次结构中，群集中集群设置。 对于层次结构中的每个簇，计算基于簇内项目的特征向量的代表向量。 计算和使用用户问题的特征向量以及层次结构中每个级别的代表向量，以遍历和导航集群层次结构。 当找到叶子簇时，叶群中的项目（如先前输入的问题）返回给用户。 可以基于期望返回的项目数量来选择叶子集群中的项目子集或分支集群中其他叶子集群的项目。

    Kits for Isolating Nucleic Acids Using Multifunctional Group-Coated Solid Phase Carriers
    3.
    发明申请
    Kits for Isolating Nucleic Acids Using Multifunctional Group-Coated Solid Phase Carriers 审中-公开
    使用多功能组涂固相载体分离核酸的试剂盒

    公开(公告)号：US20090191566A1

    公开(公告)日：2009-07-30

    申请号：US12396586

    申请日：2009-03-03

    Applicant: Kevin J. McKernan ,  Erik Gustafson ,  Adrianne D. Brand

    Inventor： Kevin J. McKernan ,  Erik Gustafson ,  Adrianne D. Brand

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q2527/125

    Abstract: The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e.g., buffer) that selectively removes (e.g., elutes) either the nucleic acid from the first functional group or the target nucleic acid species from the second functional group of the solid phase carriers, thereby isolating a target nucleic acid species from a mixture comprising a plurality of nucleic acid species.

    Abstract translation: 本发明涉及从混合物中分离靶物种（例如靶核酸物种）的方法。 在本发明的方法中，将混合物与具有包含多个官能团的表面的固相载体组合，其中一个可逆地和选择性地结合靶物种。 在一个具体实施方案中，将混合物与具有可逆结合核酸的第一官能团的固相载体和选择性和可逆结合靶核酸物质的第二官能团组合，从而产生第一组合。 第一组合在适于将核酸与第一官能团结合并且靶核酸物质与第二官能团结合的条件下保持。 将固相载体与第一组合分离，并与选择性地除去（例如，洗脱）来自第一官能团的核酸或来自第二官能团的第二官能团的靶核酸物质的试剂（例如，缓冲液）组合 固相载体，从而从包含多个核酸物质的混合物中分离靶核酸物质。

    Economizer biasing valve for cryogenic fluids
    6.
    发明授权
    Economizer biasing valve for cryogenic fluids 有权
    节能阀用于低温流体

    公开(公告)号：US09581295B2

    公开(公告)日：2017-02-28

    申请号：US13413104

    申请日：2012-03-06

    Applicant: Erik Gustafson

    Inventor： Erik Gustafson

    IPC: F17C7/04

    CPC classification number: F17C7/04 ,  F17C2201/0109 ,  F17C2201/032 ,  F17C2201/035 ,  F17C2205/0326 ,  F17C2205/0332 ,  F17C2205/0335 ,  F17C2205/0382 ,  F17C2221/033 ,  F17C2223/0161 ,  F17C2223/043 ,  F17C2223/046 ,  F17C2227/0107 ,  F17C2227/0393 ,  F17C2270/0168

    Abstract: Described herein are systems and methods for cryogenic fluid delivery. The systems may include a pressure vessel containing a cryogenic fluid formed of liquid and vapor that is connected to a use device via a withdrawal line. The withdrawal line connects to the cryogenic fluid in the pressure vessel via two routes, a liquid tube and a vapor line. The vapor line may include a back-pressure regulator that opens the vapor line depending on pressure in the system. The withdrawal line may include a pressure relief valve that exerts pressure on the liquid tube. A bypass line may connect the withdrawal line to the liquid tube. The bypass line has a check valve that permits free flow of cryogen from the withdrawal line to the liquid tube via the bypass line while prohibiting cryogen flow from the pressure vessel through the bypass line. The methods employ the systems described herein.

    Abstract translation: 本文描述了用于低温流体递送的系统和方法。 该系统可以包括压力容器，其包含由液体和蒸气形成的低温流体，该低温流体经由抽出管线连接到使用装置。 抽出管线通过两条路线连接压力容器中的低温流体，即液体管道和蒸汽管线。 蒸气管线可以包括背压调节器，其根据系统中的压力打开蒸气管线。 抽出管线可以包括对液体管施加压力的压力释放阀。 旁路管路可以将抽出管线连接到液体管道。 旁路管路具有止回阀，其允许冷冻剂通过旁路管线从排出管线自由流动到液体管道，同时禁止低温冷却剂从压力容器流过旁通管线。 该方法采用本文描述的系统。

    ECONOMIZER BIASING VALVE FOR CRYOGENIC FLUIDS
    8.
    发明申请
    ECONOMIZER BIASING VALVE FOR CRYOGENIC FLUIDS 有权
    用于低温流体的经济型偏心阀

    公开(公告)号：US20130232997A1

    公开(公告)日：2013-09-12

    申请号：US13413104

    申请日：2012-03-06

    Applicant: Erik Gustafson

    Inventor： Erik Gustafson

    IPC: F17C7/04

    CPC classification number: F17C7/04 ,  F17C2201/0109 ,  F17C2201/032 ,  F17C2201/035 ,  F17C2205/0326 ,  F17C2205/0332 ,  F17C2205/0335 ,  F17C2205/0382 ,  F17C2221/033 ,  F17C2223/0161 ,  F17C2223/043 ,  F17C2223/046 ,  F17C2227/0107 ,  F17C2227/0393 ,  F17C2270/0168

    Abstract: Described herein are systems and methods for cryogenic fluid delivery. The systems may include a pressure vessel containing a cryogenic fluid formed of liquid and vapor that is connected to a use device via a withdrawal line. The withdrawal line connects to the cryogenic fluid in the pressure vessel via two routes, a liquid tube and a vapor line. The vapor line may include a back-pressure regulator that opens the vapor line depending on pressure in the system. The withdrawal line may include a pressure relief valve that exerts pressure on the liquid tube. A bypass line may connect the withdrawal line to the liquid tube. The bypass line has a check valve that permits free flow of cryogen from the withdrawal line to the liquid tube via the bypass line while prohibiting cryogen flow from the pressure vessel through the bypass line. The methods employ the systems described herein.

    Abstract translation: 本文描述了用于低温流体递送的系统和方法。 该系统可以包括压力容器，其包含由液体和蒸气形成的低温流体，该低温流体经由抽出管线连接到使用装置。 抽出管线通过两条路线连接压力容器中的低温流体，即液体管道和蒸汽管线。 蒸气管线可以包括背压调节器，其根据系统中的压力打开蒸气管线。 抽出管线可以包括对液体管施加压力的压力释放阀。 旁路管路可以将抽出管线连接到液体管道。 旁路管路具有止回阀，其允许冷冻剂通过旁路管线从排出管线自由流动到液体管道，同时禁止低温冷却剂从压力容器流过旁通管线。 该方法采用本文描述的系统。

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