公开(公告)号：US20180243665A1

公开(公告)日：2018-08-30

申请号：US15753341

申请日：2016-08-18

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Karol Maciej Lacki ,  Hans Blom ,  Helena Skoglar ,  Linus Laurin

IPC: B01D15/38 ,  B01J20/24 ,  B01J20/286 ,  C07K1/22 ,  B01J20/28 ,  B01J20/32 ,  C07K16/06

CPC classification number: B01D15/3809 ,  B01J20/24 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28019 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3274 ,  B01J20/3278 ,  B01J20/3293 ,  B01J2220/603 ,  C07K1/22 ,  C07K16/065

Abstract: The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 μm. The invention further discloses a method of separation of antibodies by affinity chromatography which employs the said separation matrix within a chromatography column.