公开(公告)号：US20200317725A1

公开(公告)日：2020-10-08

申请号：US16911511

申请日：2020-06-25

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC: C07K1/16 ,  C07K16/00 ,  B01J20/286 ,  B01J20/285 ,  B01D15/18 ,  B01D15/34 ,  C07K1/22 ,  C07K14/31

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US20180044374A1

公开(公告)日：2018-02-15

申请号：US15798784

申请日：2017-10-31

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC: C07K1/16 ,  B01D15/18 ,  B01J20/285 ,  B01D15/34 ,  C07K16/00 ,  B01J20/286

CPC classification number: C07K1/165 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/34 ,  B01J20/285 ,  B01J20/286 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2319/30

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US10730908B2

公开(公告)日：2020-08-04

申请号：US15798784

申请日：2017-10-31

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC: C07K1/16 ,  B01D15/08 ,  C07K1/22 ,  C07K16/00 ,  B01J20/286 ,  B01J20/285 ,  B01D15/18 ,  B01D15/34 ,  C07K14/31

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US20180243665A1

公开(公告)日：2018-08-30

申请号：US15753341

申请日：2016-08-18

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Karol Maciej Lacki ,  Hans Blom ,  Helena Skoglar ,  Linus Laurin

IPC: B01D15/38 ,  B01J20/24 ,  B01J20/286 ,  C07K1/22 ,  B01J20/28 ,  B01J20/32 ,  C07K16/06

CPC classification number: B01D15/3809 ,  B01J20/24 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28019 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3274 ,  B01J20/3278 ,  B01J20/3293 ,  B01J2220/603 ,  C07K1/22 ,  C07K16/065

Abstract: The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 μm. The invention further discloses a method of separation of antibodies by affinity chromatography which employs the said separation matrix within a chromatography column.