公开(公告)号：US20180127460A1

公开(公告)日：2018-05-10

申请号：US15564232

申请日：2016-04-04

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Martin Hall ,  Karolina Busson ,  Jean-Luc Maloisel ,  Helena Skoglar

IPC: C07K1/18 ,  B01D15/36 ,  B01D15/38 ,  B01D15/34 ,  C12N9/64 ,  C07K14/755 ,  C07K16/00 ,  C07K14/765 ,  C07K14/47 ,  B01J41/20

CPC classification number: C07K1/18 ,  A61K35/16 ,  A61K38/00 ,  A61K38/36 ,  A61K38/4846 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3847 ,  B01J41/20 ,  C07K14/472 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00 ,  C12N9/6424 ,  C12N9/644 ,  C12Y304/21022

Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of plasmaproteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

公开(公告)号：US20160367966A1

公开(公告)日：2016-12-22

申请号：US15120845

申请日：2015-02-18

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Jean-Luc Maloisel ,  Karolina Busson ,  Karol Lacki ,  Bjorn Noren ,  Helena Skoglar

IPC: B01J20/32 ,  B01J20/28 ,  B01D15/20 ,  B01D15/30 ,  C12N1/20 ,  B01D15/12 ,  C07K1/22 ,  C07K14/765 ,  C07K14/77 ,  B01J20/286 ,  B01D15/36

CPC classification number: B01J20/3293 ,  B01D15/125 ,  B01D15/203 ,  B01D15/305 ,  B01D15/363 ,  B01J20/28019 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/327 ,  B01J20/3278 ,  C07K1/22 ,  C07K14/765 ,  C07K14/77 ,  C12N1/20

Abstract: The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.

Abstract translation: 本发明公开了一种用于纯化生物颗粒的分离基质，其包含多个具有多孔核心实体和覆盖核心实体的多孔壳体的颗粒，其中核心实体包含共价连接的配体上存在的至少50微摩尔/ 每个配体显示至少两个伯胺，壳体包含少于20微摩尔/ ml伯胺本发明还公开了一种纯化生物颗粒的方法和一种制备分离基质的方法。

公开(公告)号：US20180243665A1

公开(公告)日：2018-08-30

申请号：US15753341

申请日：2016-08-18

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Karol Maciej Lacki ,  Hans Blom ,  Helena Skoglar ,  Linus Laurin

IPC: B01D15/38 ,  B01J20/24 ,  B01J20/286 ,  C07K1/22 ,  B01J20/28 ,  B01J20/32 ,  C07K16/06

CPC classification number: B01D15/3809 ,  B01J20/24 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28019 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3274 ,  B01J20/3278 ,  B01J20/3293 ,  B01J2220/603 ,  C07K1/22 ,  C07K16/065

Abstract: The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 μm. The invention further discloses a method of separation of antibodies by affinity chromatography which employs the said separation matrix within a chromatography column.