公开(公告)号：US20160376550A1

公开(公告)日：2016-12-29

申请号：US15262644

申请日：2016-09-12

Applicant: General Electric Company

Inventor： Reginald Donovan Smith ,  Prameela Susarla ,  Slawomir Rubinsztajn ,  Brian David Polizzotti ,  Anton Beletskii

IPC: C12N5/00 ,  C12M1/26 ,  C08F8/12 ,  C12M1/00

CPC classification number: C12N5/0075 ,  C08F8/12 ,  C08F293/005 ,  C12M23/20 ,  C12M33/00 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2539/10

Abstract: A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.

Abstract translation: 一种细胞培养支持物，其包含底物和固定在底物上的双重刺激响应性嵌段共聚物，其中所述双重刺激响应性嵌段共聚物是热反应性和pH响应性的。 一种培养细胞的方法，所述细胞包含具有固定在底物上的双重刺激响应共聚物的细胞培养支持物，其中所述双重刺激响应共聚物是热反应性和pH响应性的; 并在细胞培养支持下培养细胞。 通过降低温度，细胞从细胞培养支持物释放。

公开(公告)号：US10131874B2

公开(公告)日：2018-11-20

申请号：US15262644

申请日：2016-09-12

Applicant: General Electric Company

Inventor： Reginald Donovan Smith ,  Prameela Susarla ,  Slawomir Rubinsztajn ,  Brian David Polizzotti ,  Anton Beletskii

IPC: C12N5/00 ,  C08F293/00 ,  C12M1/26 ,  C12M1/00 ,  C08F8/12

Abstract: A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.

公开(公告)号：US20150093739A1

公开(公告)日：2015-04-02

申请号：US14565142

申请日：2014-12-09

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Nichole Lea Wood ,  Reginald Donovan Smith ,  Hongyi Zhou ,  Liming Yu ,  Brian David Polizzotti ,  Peter D. Miller

IPC: G01N33/49 ,  C12N5/0775 ,  G01N1/40 ,  C12N5/0789

CPC classification number: G01N33/491 ,  A61M1/029 ,  B01D61/28 ,  B01D61/30 ,  B01D63/02 ,  B01D2313/18 ,  B01D2313/50 ,  C12N5/0647 ,  C12N5/0665 ,  G01N1/4077 ,  G01N2001/4083 ,  G01N2001/4088

Abstract: Method and systems, for processing biological material, that contain a biological material in a vessel; add an aggregating agent to the material in the vessel and allow the material to separate into two or more distinct submaterials; extract one or more of the submaterials from the vessel; automatically transport one or more of the submaterials remaining in the vessel to a filtration device; and collect a resulting target retentate into a target retentate receptacle.

Abstract translation: 用于处理在容器中含有生物材料的生物材料的方法和系统; 向容器中的材料添加聚集剂，并使材料分离成两个或更多不同的子材料; 从容器中提取一种或多种副材料; 将剩余在容器中的一种或多种副材料自动输送到过滤装置; 并将得到的目标滞留物收集到目标滞留容器中。

公开(公告)号：US09709549B2

公开(公告)日：2017-07-18

申请号：US14565142

申请日：2014-12-09

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Nichole Lea Wood ,  Reginald Donovan Smith ,  Hongyi Zhou ,  Liming Yu ,  Brian David Polizzotti ,  Peter D. Miller

IPC: G01N33/49 ,  A61M1/02 ,  B01D61/28 ,  B01D61/30 ,  B01D63/02 ,  C12N5/0789 ,  C12N5/0775 ,  G01N1/40

CPC classification number: G01N33/491 ,  A61M1/029 ,  B01D61/28 ,  B01D61/30 ,  B01D63/02 ,  B01D2313/18 ,  B01D2313/50 ,  C12N5/0647 ,  C12N5/0665 ,  G01N1/4077 ,  G01N2001/4083 ,  G01N2001/4088

Abstract: Method and systems, for processing biological material, that contain a biological material in a vessel; add an aggregating agent to the material in the vessel and allow the material to separate into two or more distinct submaterials; extract one or more of the submaterials from the vessel; automatically transport one or more of the submaterials remaining in the vessel to a filtration device; and collect a resulting target retentate into a target retentate receptacle.