-
公开(公告)号：US20120100527A1
公开(公告)日：2012-04-26
申请号：US13232091
申请日：2011-09-14
申请人： Harumi Minekawa , Tsugunori Notomi , Toshihiro Yonekawa , Norihiro Tomita , Yoko Kuzuhara , Takato Odagiri
发明人： Harumi Minekawa , Tsugunori Notomi , Toshihiro Yonekawa , Norihiro Tomita , Yoko Kuzuhara , Takato Odagiri
IPC分类号： C12Q1/70
CPC分类号： C12Q1/701
摘要： The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis.
摘要翻译： 本发明提供与由H5或H7禽流感病毒的血凝素的核苷酸序列设计的任意核苷酸序列特异性杂交的寡核苷酸引物，使用引物的核酸扩增方法，用H5或H7禽流感感染诊断的方法 检测核酸扩增的流感病毒，以及用于流感诊断的试剂盒。
-
公开(公告)号：US08043813B2
公开(公告)日：2011-10-25
申请号：US11718357
申请日：2005-10-26
申请人： Harumi Minekawa , Tsugunori Notomi , Toshihiro Yonekawa , Norihiro Tomita , Yoko Kuzuhara , Takato Odagiri
发明人： Harumi Minekawa , Tsugunori Notomi , Toshihiro Yonekawa , Norihiro Tomita , Yoko Kuzuhara , Takato Odagiri
CPC分类号： C12Q1/701
摘要： The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis.
摘要翻译： 本发明提供与由H5或H7禽流感病毒的血凝素的核苷酸序列设计的任意核苷酸序列特异性杂交的寡核苷酸引物，使用引物的核酸扩增方法，用H5或H7禽流感感染诊断的方法 检测核酸扩增的流感病毒，以及用于流感诊断的试剂盒。
-
公开(公告)号：US20090317795A1
公开(公告)日：2009-12-24
申请号：US11718357
申请日：2005-10-26
申请人： Harumi Minekawa , Tsugunori Notomi , Toshihiro Yonekawa , Norihiro Tomita , Yoko Kuzuhara , Takato Odagiri
发明人： Harumi Minekawa , Tsugunori Notomi , Toshihiro Yonekawa , Norihiro Tomita , Yoko Kuzuhara , Takato Odagiri
CPC分类号： C12Q1/701
摘要： The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis.
摘要翻译： 本发明提供与由H5或H7禽流感病毒的血凝素的核苷酸序列设计的任意核苷酸序列特异性杂交的寡核苷酸引物，使用引物的核酸扩增方法，用H5或H7禽流感感染诊断的方法 检测核酸扩增的流感病毒，以及用于流感诊断的试剂盒。
-
公开(公告)号：US08389221B2
公开(公告)日：2013-03-05
申请号：US13232091
申请日：2011-09-14
CPC分类号： C12Q1/701
摘要： The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis.
摘要翻译： 本发明提供与由H5或H7禽流感病毒的血凝素的核苷酸序列设计的任意核苷酸序列特异性杂交的寡核苷酸引物，使用引物的核酸扩增方法，用H5或H7禽流感感染诊断的方法 检测核酸扩增的流感病毒，以及用于流感诊断的试剂盒。
-
公开(公告)号：US07175985B1
公开(公告)日：2007-02-13
申请号：US10129637
申请日：2002-08-02
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6827 , C12Q1/6858 , Y02A50/58 , C12Q2537/1373 , C12Q2531/101 , C12Q2531/119 , C12Q2525/161
摘要： The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3′-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.
摘要翻译： 本发明的方法是基于这样一种方法，其中使用通过互补链合成合成的杂交3'-末端在存在于其核苷酸序列的靶核苷酸序列的特定区域上合成核酸 链作为下一轮互补链合成的起源。 根据本发明重复进行与特定区域的杂交，该区域被搜索突变和多态性。 因此，靶核苷酸序列中的突变和多态性被精确地复制到反应产物中。
-
公开(公告)号：US07638280B2
公开(公告)日：2009-12-29
申请号：US11615742
申请日：2006-12-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , Y02A50/58
摘要： The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3′-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.
摘要翻译： 本发明的方法是基于这样一种方法，其中使用通过互补链合成合成的杂交3'-末端在存在于其核苷酸序列的靶核苷酸序列的特定区域上合成核酸 链作为下一轮互补链合成的起源。 根据本发明重复进行与特定区域的杂交，该区域被搜索突变和多态性。 因此，靶核苷酸序列中的突变和多态性被精确地复制到反应产物中。
-
公开(公告)号：US20070238113A1
公开(公告)日：2007-10-11
申请号：US11615742
申请日：2006-12-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , Y02A50/58
摘要： The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3′-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.
摘要翻译： 本发明的方法是基于这样一种方法，其中使用通过互补链合成合成的杂交3'-末端在存在于其核苷酸序列的靶核苷酸序列的特定区域上合成核酸 链作为下一轮互补链合成的起源。 根据本发明重复进行与特定区域的杂交，该区域被搜索突变和多态性。 因此，靶核苷酸序列中的突变和多态性被精确地复制到反应产物中。
-
8.发明授权公开(公告)号：US08557523B2
公开(公告)日：2013-10-15
申请号：US12738413
申请日：2008-10-17
CPC分类号： C12Q1/6876 , C12Q1/6851 , C12Q2561/113 , C12Q2527/143 , C12Q2527/113 , C12Q2545/101 , C12Q2537/149 , C12Q2531/119
摘要： The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.
摘要翻译： 本发明的目的是提供一种允许内标物质稳定扩增的方法，同时在涉及使用内标物质和使用的试剂盒的核酸检测系统中保持目标核酸的准确测定值 因此。 本发明涉及一种用于核酸扩增的方法，包括通过在靶核酸扩增方法中扩增靶核酸之前，通过扩增内标物质来防止内标扩增产物影响靶核酸的扩增反应 使用内标物质的样品中的核酸和用于其的试剂和试剂盒。
-
9.发明申请METHOD OF NUCLEIC ACID AMPLIFICATION AND MEASURING REAGENT AND REAGENT KIT THEREFOR 失效核酸扩增方法及其测试试剂及其试剂盒公开(公告)号：US20100233715A1
公开(公告)日：2010-09-16
申请号：US12738413
申请日：2008-10-17
CPC分类号： C12Q1/6876 , C12Q1/6851 , C12Q2561/113 , C12Q2527/143 , C12Q2527/113 , C12Q2545/101 , C12Q2537/149 , C12Q2531/119
摘要： The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.
摘要翻译： 本发明的目的是提供一种允许内标物质稳定扩增的方法，同时在涉及使用内标物质和使用的试剂盒的核酸检测系统中保持目标核酸的准确测定值 因此。 本发明涉及一种用于核酸扩增的方法，包括通过在靶核酸扩增方法中扩增靶核酸之前，通过扩增内标物质来防止内标扩增产物影响靶核酸的扩增反应 使用内标物质的样品中的核酸和用于其的试剂和试剂盒。
-
公开(公告)号：US5922544A
公开(公告)日：1999-07-13
申请号：US849701
申请日：1998-02-09
CPC分类号： C12Q1/6888
摘要： A method and a reagent for detecting cells of a living organism contained in various samples of the organism by detecting genes specific for each animal species. This method is useful for detecting bleeding into feces. It is possible for confirming the bleeding of the human body to use an Alu sequence as the sequence specific for the animal species. As genes are used as the indicator in this method, fecal occult blood can be detected more specifically at a higher sensitivity than by the conventional technique using hemoglobin as the indicator.
摘要翻译： PCT No.PCT / JP95 / 02734 Sec。 371日期：1998年2月9日 102（e）1998年2月9日PCT 1995年12月27日PCT PCT。 公开号WO96 / 21041 日期1996年7月11日通过检测每种动物物种特异性的基因，检测生物体的各种样品中所含活体的细胞的方法和试剂。 该方法可用于检测粪便中的出血。 可以确认人体出血以使用Alu序列作为动物物种特有的序列。 由于基因作为该方法中的指示剂，所以能够以比使用血红蛋白作为指标的常规技术更高的灵敏度检测出粪便潜血。
-
-
-
-
-
-
-
-
-
搜索结果
10 条记录 (耗时 0.024 秒)
