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    Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacteria
    1.
    发明授权
    Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacteria 有权
    基因参与乙酸耐量，使用该基因繁殖的乙酸菌，以及使用乙酸菌生产醋的方法

    公开(公告)号：US07446192B2

    公开(公告)日：2008-11-04

    申请号：US10507446

    申请日：2003-03-12

    申请人： Hidetsugu Goto ,  Shigeru Nakano

    发明人： Hidetsugu Goto ,  Shigeru Nakano

    IPC分类号： C07H21/04

    CPC分类号： C12J1/04 ,  C07K14/195 ,  C12N1/20

    摘要： The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher concentration by using acetic acid bacteria whose acetic acid tolerance is improved. In the present invention, novel genes having a function for improving acetic acid tolerance on practical level were cloned from practical acetic acid bacteria belonging to the genus Gluconacetobacter by a method of obtaining genes from chromosomal DNA library that enable to grow on the medium at a high concentration of acetic acid. Further, in transformants in which the genes were introduced into acetic acid bacteria, acetic acid tolerance was remarkably increased, and when the transformants are subjected to aeration culture in the presence of ethanol, the growth lag-time can be shortened, and the growth rate can also be improved, moreover the final acetic acid concentration can be remarkably improved.

    摘要翻译： 本发明旨在提供参与乙酸菌的乙酸耐受性的新基因，以及通过使用该基因来提高微生物，特别是乙酸菌的乙酸耐受性的方法，还提出了一种有效生产乙酸醋的方法 通过使用醋酸耐受性提高的乙酸菌，浓度较高的酸。 在本发明中，通过从染色体DNA文库获得能够在培养基上高生长的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平提高乙酸耐受性的新型基因 乙酸浓度。 此外，在将基因导入乙酸菌的转化体中，乙酸耐受性显着增加，当转化体在乙醇存在下进行通气培养时，可以缩短生长滞后时间，生长速度 也可以改善，而且可以显着提高最终的乙酸浓度。

    Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacterium
    2.
    发明申请
    Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacterium 有权
    基因参与乙酸耐量，使用该基因繁殖的乙酸菌，以及使用乙酸菌生产醋的方法

    公开(公告)号：US20060154351A1

    公开(公告)日：2006-07-13

    申请号：US10507446

    申请日：2003-03-12

    申请人： Hidetsugu Goto ,  Shigeru Nakano

    发明人： Hidetsugu Goto ,  Shigeru Nakano

    IPC分类号： C12P7/54 ,  C12N9/02 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12J1/04 ,  C07K14/195 ,  C12N1/20

    摘要： The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher concentration by using acetic acid bacteria whose acetic acid tolerance is improved. In the present invention, novel genes having a function for improving acetic acid tolerance on practical level were cloned from practical acetic acid bacteria belonging to the genus Gluconacetobacter by a method of obtaining genes from chromosomal DNA library that enable to grow on the medium at a high concentration of acetic acid. Further, in transformants in which the genes were introduced into acetic acid bacteria, acetic acid tolerance was remarkably increased, and when the transformants are subjected to aeration culture in the presence of ethanol, the growth lag-time can be shortened, and the growth rate can also be improved, moreover the final acetic acid concentration can be remarkably improved.

    摘要翻译： 本发明旨在提供参与乙酸菌的乙酸耐受性的新基因，以及通过使用该基因来提高微生物，特别是乙酸菌的乙酸耐受性的方法，还提出了一种有效生产乙酸醋的方法 通过使用醋酸耐受性提高的乙酸菌，浓度较高的酸。 在本发明中，通过从染色体DNA文库获得能够在培养基上高生长的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平提高乙酸耐受性的新型基因 乙酸浓度。 此外，在将基因导入乙酸菌的转化体中，乙酸耐受性显着增加，当转化体在乙醇存在下进行通气培养时，可以缩短生长滞后时间，生长速度 也可以改善，而且可以显着提高最终的乙酸浓度。

    METHOD FOR PRODUCING COMPOSITION CONTAINING SIALIC ACID COMPOUND
    3.
    发明申请
    METHOD FOR PRODUCING COMPOSITION CONTAINING SIALIC ACID COMPOUND 审中-公开
    生产含有酸性化合物的组合物的方法

    公开(公告)号：US20100143535A1

    公开(公告)日：2010-06-10

    申请号：US12452452

    申请日：2008-07-04

    申请人： Hidemasa Motoshima ,  Hidetsugu Goto ,  Nozomi Ueda ,  Yoshiaki Ookubo

    发明人： Hidemasa Motoshima ,  Hidetsugu Goto ,  Nozomi Ueda ,  Yoshiaki Ookubo

    IPC分类号： C12P19/26 ,  C12G3/02

    CPC分类号： A61K31/7012 ,  A61K35/20 ,  C12G3/02 ,  C12P19/26

    摘要： Provided is a method of efficiently and economically producing a sialic acid compound-containing composition, in which a content rate of a sialic acid compound is remarkably high, from natural raw materials containing a sialic acid compound such as milk, whey, or a processed product thereof. The present invention provides a method of producing a sialic acid compound-containing composition, comprising: using, as a raw material, a liquid raw material containing a sialic acid compound or a liquid in which a solid raw material containing the sialic acid compound is dissolved or suspended in water; culturing, in the raw material, a microorganism which cannot assimilate the sialic acid compound and can assimilate carbohydrates other than the sialic acid compound contained in the raw material; allowing the microorganism to assimilate the carbohydrates other than the sialic acid compound contained in the raw material; and removing the microorganism.

    摘要翻译： 本发明提供一种从含有唾液酸化合物如牛奶，乳清或加工产品的天然原料中有效且经济地制备唾液酸化合物含量高的组合物，其中唾液酸化合物的含量显着高 其中。 本发明提供含有唾液酸化合物的组合物的制造方法，其特征在于，以含有唾液酸化合物或含有唾液酸化合物的固体原料溶解的液体的原料为原料 或悬浮在水中; 在原料中培养不能吸收唾液酸化合物并且可以吸收除原料中所含的唾液酸化合物以外的碳水化合物的微生物; 允许微生物吸收除原料中所含的唾液酸化合物以外的碳水化合物; 并除去微生物。