公开(公告)号：US20220205993A1

公开(公告)日：2022-06-30

申请号：US17560276

申请日：2021-12-23

Applicant: Industrial Technology Research Institute

Inventor： Wen-Ting Chiang ,  Chien-An Chen ,  Cheng-Tai Chen

IPC: G01N33/543 ,  G01N33/58 ,  G01N33/53

Abstract: A detection method of multiple analytes includes the following. A microparticle is provided. The microparticle is coupled with at least one first ligand, and includes a body and a plurality of first protrusions formed on a surface of the body. Next, the microparticle is mixed with a variety of analytes to form a first complex. Thereafter, the first complex is mixed with a variety of second ligands carrying a variety of first labels, such that the variety of second ligands bind to the variety of analytes in the first complex and form a second complex. Lastly, the variety of first labels in the variety of second ligands in the second complex are detected.

公开(公告)号：US20240218310A1

公开(公告)日：2024-07-04

申请号：US18089570

申请日：2022-12-28

Applicant: Industrial Technology Research Institute

Inventor： Li-An Wu ,  Ting-Hsuan Chen ,  Chih-Hung Lee ,  Nien-Jen Chou ,  Kuo-Hsing Wen ,  Wen-Ting Chiang

IPC: C12M1/06 ,  C12M1/00 ,  C12M1/12 ,  C12M3/00 ,  C12M3/06

CPC classification number: C12M27/02 ,  C12M23/16 ,  C12M23/38 ,  C12M23/48 ,  C12M29/04 ,  C12M37/04

Abstract: A cell culture device configured for activating, transducing, isolating and/or expanding cells. The cell culture device includes a mixing tank and a rotary control platform. The mixing tank includes a first support, a hollow tank and a cover. The hollow tank is disposed on the first support and has a first opening, a second opening and an accommodating space. The cover covers the second opening and has a first hole, an inlet end and an outlet end. The rotary control platform includes a base, a second support, a rotating gear, an inclination control member and a micro injector. The second support is disposed on the base. The rotating gear is fixed on the first support. The inclination control member has a first part, a second part and a third part. The first part is connected to the rotating gear, and the second part is connected to the first part and the third part. The micro injector extends to the accommodating space through the first hole of the cover. The micro injector has a top end and a bottom end, the top end is fixed on the third part, and the bottom end is disposed to a bottom of the accommodating space. The micro injector is inclined towards the bottom of the accommodating space.

公开(公告)号：US11859176B2

公开(公告)日：2024-01-02

申请号：US16228763

申请日：2018-12-21

Applicant: Industrial Technology Research Institute ,  National Taiwan University Hospital

Inventor： Cheng-Tai Chen ,  Chien-An Chen ,  Wen-Ting Chiang ,  Su-Feng Chiu ,  Pei-Shin Jiang ,  Jih-Luh Tang ,  Chien-Ting Lin ,  Xuan-Hui Lin

IPC: C12N5/00 ,  C12N13/00 ,  G01N33/543 ,  C12N5/0783 ,  C12N5/078 ,  A61K9/14

CPC classification number: C12N13/00 ,  C12N5/0634 ,  C12N5/0636 ,  C12N5/0646 ,  G01N33/54333 ,  C12N2501/505 ,  C12N2501/515 ,  C12N2501/998 ,  C12N2529/00 ,  C12N2533/10 ,  C12N2533/30 ,  C12N2535/00 ,  C12N2535/10

Abstract: A method for in vitro activation and/or expansion of immune cells is provided, including the steps of: a) providing magnetic particles having multi-protrusive surface modified with at least one type of immuno-inducing substance, in which each magnetic particle includes a copolymer core, a polymer layer, a magnetic substance layer, and a silicon-based layer from the inside to the outside; b) providing a cell solution including at least one type of immune cell in the cell solution; and c) bringing the magnetic particles in contact with the cell solution, in which the at least one type of immuno-inducing substance on the surface of the magnetic particle activates and/or expands the at least one type of immune cell in the cell solution.

公开(公告)号：US20190203195A1

公开(公告)日：2019-07-04

申请号：US16228763

申请日：2018-12-21

Applicant: Industrial Technology Research Institute ,  National Taiwan University Hospital

Inventor： Cheng-Tai Chen ,  Chien-An Chen ,  Wen-Ting Chiang ,  Su-Feng Chiu ,  Pei-Shin Jiang ,  Jih-Luh Tang ,  Chien-Ting Lin ,  Xuan-Hui Lin

IPC: C12N13/00 ,  C12N5/078

CPC classification number: C12N13/00 ,  C12N5/0634 ,  C12N5/0636 ,  C12N2501/505 ,  C12N2501/515 ,  C12N2501/998 ,  C12N2529/00 ,  C12N2533/10 ,  C12N2533/30 ,  C12N2535/00 ,  C12N2535/10 ,  G01N33/54333

Abstract: A method for in vitro activation and/or expansion of immune cells is provided, including the steps of: a) providing magnetic particles having multi-protrusive surface modified with at least one type of immuno-inducing substance, in which each magnetic particle includes a copolymer core, a polymer layer, a magnetic substance layer, and a silicon-based layer from the inside to the outside; b) providing a cell solution including at least one type of immune cell in the cell solution; and c) bringing the magnetic particles in contact with the cell solution, in which the at least one type of immuno-inducing substance on the surface of the magnetic particle activates and/or expands the at least one type of immune cell in the cell solution.