公开(公告)号：US20170145466A1

公开(公告)日：2017-05-25

申请号：US15323144

申请日：2015-07-02

Applicant: KANEKA CORPORATION

Inventor： Masutoshi NOJIRI ,  Yoshihiko YASOHARA

IPC: C12P21/02

CPC classification number: C12P21/02 ,  C12N9/00

Abstract: The technical problem to be solved by the present invention is to provide a method for producing oxidized glutathione, GSSG and a precursor thereof, i.e., oxidized γ-glutamylcysteine, by a simple process. As a means for solving the problem, the method for producing GSSG according to the present invention comprises step A′ of reacting L-cystine and L-glutamic acid to produce oxidized γ-glutamylcysteine and step B′ of reacting oxidized γ-glutamylcysteine and glycine to produce GSSG.

公开(公告)号：US20160040138A1

公开(公告)日：2016-02-11

申请号：US14780419

申请日：2014-03-25

Applicant: KANEKA CORPORATION

Inventor： Misato MATSUI ,  Noriyuki ITO ,  Shigeru KAWANO ,  Yoshihiko YASOHARA

IPC: C12N9/04 ,  C12P7/04

CPC classification number: C12N9/0006 ,  C12P7/02 ,  C12P7/04 ,  C12Y101/01184

Abstract: An object of the present invention is to modify a wild-type enzyme that is less reactive in the presence of an organic solvent to provide altered carbonyl reductases having better reactivity in the presence of the organic solvent than the wild-type enzyme, and/or to provide transformants producing such reductases. The present inventors have found altered carbonyl reductases having better reactivity in the presence of an organic solvent than the wild-type enzyme, from among a mutant enzyme library prepared by randomly mutating the wild-type enzyme gene, thereby arriving at completion of the present invention.

Abstract translation: 本发明的目的是修饰在有机溶剂存在下反应性较低的野生型酶，以提供在有机溶剂存在下具有比野生型酶更好反应性的改变的羰基还原酶，和/或 以提供生产这种还原酶的转化体。 本发明人发现在通过随机突变野生型酶基因制备的突变酶库中，在有机溶剂存在下比野生型酶具有更好的反应性的羰基还原酶，从而达到本发明的完成 。

公开(公告)号：US20200010814A1

公开(公告)日：2020-01-09

申请号：US16584734

申请日：2019-09-26

Applicant: KANEKA CORPORATION ,  Kyoto University

Inventor： Masutoshi NOJIRI ,  Yoshihiko YASOHARA ,  Makoto HIBI ,  Jun OGAWA

IPC: C12N9/02 ,  C12N15/63

Abstract: An enzyme protein that allows production of an optically active β-hydroxyamino acid, specifically a monooxygenase having two kinds of hetero (different) subunits. Also, systems and methods using the monooxygenases.

公开(公告)号：US20170211117A1

公开(公告)日：2017-07-27

申请号：US15325891

申请日：2015-07-28

Applicant: KANEKA CORPORATION

Inventor： Masutoshi NOJIRI ,  Yoshihiko YASOHARA

IPC: C12P21/02 ,  C12N9/12 ,  C12N9/00

CPC classification number: C12P21/02 ,  C12N9/00 ,  C12N9/1229 ,  C12N9/93 ,  C12P19/32 ,  C12Y207/04 ,  C12Y603/02002 ,  C12Y603/02003

Abstract: The technical problem to be solved by the present invention is to provide a method for producing glutathione and a precursor thereof, γ-glutamylcysteine, at a high yield. As a means for solving the problem, the method for producing glutathione according to the present invention includes step A′ of reacting L-cysteine and L-glutamic acid under a low-oxygen atmosphere to produce γ-glutamylcysteine and step B′ of reacting γ-glutamylcysteine and glycine under a low-oxygen atmosphere to produce glutathione.