公开(公告)号：US20160040138A1

公开(公告)日：2016-02-11

申请号：US14780419

申请日：2014-03-25

Applicant: KANEKA CORPORATION

Inventor： Misato MATSUI ,  Noriyuki ITO ,  Shigeru KAWANO ,  Yoshihiko YASOHARA

IPC: C12N9/04 ,  C12P7/04

CPC classification number: C12N9/0006 ,  C12P7/02 ,  C12P7/04 ,  C12Y101/01184

Abstract: An object of the present invention is to modify a wild-type enzyme that is less reactive in the presence of an organic solvent to provide altered carbonyl reductases having better reactivity in the presence of the organic solvent than the wild-type enzyme, and/or to provide transformants producing such reductases. The present inventors have found altered carbonyl reductases having better reactivity in the presence of an organic solvent than the wild-type enzyme, from among a mutant enzyme library prepared by randomly mutating the wild-type enzyme gene, thereby arriving at completion of the present invention.

Abstract translation: 本发明的目的是修饰在有机溶剂存在下反应性较低的野生型酶，以提供在有机溶剂存在下具有比野生型酶更好反应性的改变的羰基还原酶，和/或 以提供生产这种还原酶的转化体。 本发明人发现在通过随机突变野生型酶基因制备的突变酶库中，在有机溶剂存在下比野生型酶具有更好的反应性的羰基还原酶，从而达到本发明的完成 。

公开(公告)号：US20230304061A1

公开(公告)日：2023-09-28

申请号：US17918857

申请日：2021-04-23

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Nobuhiko DOJUN ,  Misato MATSUI ,  Naoaki TAOKA

IPC: C12P21/02 ,  C12N9/10 ,  C12N9/48 ,  C12N9/02 ,  C12N9/88

CPC classification number: C12P21/02 ,  C12N9/0051 ,  C12N9/1029 ,  C12N9/485 ,  C12N9/88 ,  C12Y108/01007 ,  C12Y203/0103 ,  C12Y304/11004 ,  C12Y304/19013 ,  C12Y401/99001

Abstract: This invention is intended to improve glutathione productivity by fermentation using microorganisms. This invention relates to a microbial strain capable of overexpression of γ-glutamylcysteine, bis-γ-glutamylcystine, γ-glutamylcystine, reduced glutathione, and/or oxidized glutathione in which the expression level of a gene encoding serine-O-acetyltransferase (EC:2.3.1.30) is enhanced and a method involving the use of such microbial strain.

公开(公告)号：US20220290203A1

公开(公告)日：2022-09-15

申请号：US17749857

申请日：2022-05-20

Applicant: KANEKA CORPORATION

Inventor： Misato MATSUI ,  Shingo KOBAYASHI ,  Naoaki TAOKA

IPC: C12P21/02 ,  C12P13/00 ,  C12N9/10 ,  C12N9/02 ,  C12N9/00 ,  C12N9/48

Abstract: The present disclosure concerns a method for producing peptides such as glutathione and a microorganism that can be used for such method. One or more embodiments of the first aspect of the present disclosure concern a method for producing peptides such as glutathione comprising culturing a prokaryotic microbial strain in which the expression levels of one or more genes selected from among the gshA gene, the gshB gene, and the gshF gene are enhanced, compared with the expression levels thereof in the wild-type strain thereof in a medium in which the total concentration of cysteine and cystine is 0.5 g/l or lower. The second aspect of the present disclosure concerns a microorganism comprising disruptions of the γ-glutamyltransferase gene and the glutathione reductase gene and exhibiting the enhanced expression levels of the gshA gene and the gshB or gshF gene.