公开(公告)号：US10301585B2

公开(公告)日：2019-05-28

申请号：US15193962

申请日：2016-06-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeremy K. Larsen ,  Jon Q. Coleman ,  David S. Rowley

IPC: B01D29/13 ,  B01D29/27 ,  B01D35/027 ,  C12M1/00 ,  C12M1/26 ,  C12M1/12

Abstract: A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.

公开(公告)号：US12234439B2

公开(公告)日：2025-02-25

申请号：US18336553

申请日：2023-06-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeremy K. Larsen ,  Jon Q. Coleman ,  David S. Rowley

IPC: B01D29/27 ,  B01D29/13 ,  B01D35/02 ,  B01D35/027 ,  B01D61/14 ,  B01D63/06 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26

Abstract: A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.

公开(公告)号：US11840684B2

公开(公告)日：2023-12-12

申请号：US17186553

申请日：2021-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeremy K. Larsen ,  Jon Q. Coleman ,  David S. Rowley

IPC: B01D29/27 ,  B01D35/027 ,  B01D61/14 ,  B01D63/06 ,  B01D29/13 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26

CPC classification number: C12M29/04 ,  B01D29/13 ,  B01D29/27 ,  B01D35/027 ,  B01D61/147 ,  B01D63/06 ,  C12M23/14 ,  C12M23/26 ,  C12M25/16 ,  C12M33/14 ,  C12M47/02

Abstract: A method for separating microcarriers from a medium includes transferring a suspension that includes medium, microcarriers and cells into a compartment of a filter assembly. The filter assembly includes: a container having an interior surface bounding the compartment; and a filter having a tubular stem with an encircling sidewall extending between a first end and an opposing second end, the first end being secured to the interior surface of the container and the second end projecting into the compartment of the container, a plurality of openings extend through the sidewall of the stem that are large enough to allow the medium and cells to pass therethrough while preventing the microcarriers from passing therethrough. The medium and cells within the compartment are allowed to pass through the plurality of openings of the filter while the microcarriers are retained within the compartment of the container.

公开(公告)号：US20160304825A1

公开(公告)日：2016-10-20

申请号：US15193962

申请日：2016-06-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeremy K. Larsen ,  Jon Q. Coleman ,  David S. Rowley

IPC: C12M1/00 ,  B01D29/13 ,  B01D35/027 ,  C12M1/12 ,  C12M1/26

CPC classification number: C12M29/04 ,  B01D29/13 ,  B01D29/27 ,  B01D35/027 ,  C12M23/14 ,  C12M23/26 ,  C12M25/16 ,  C12M33/14 ,  C12M47/02

Abstract: A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.

公开(公告)号：US20210179995A1

公开(公告)日：2021-06-17

申请号：US17186553

申请日：2021-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeremy K. Larsen ,  Jon Q. Coleman ,  David S. Rowley

IPC: C12M1/00 ,  B01D29/27 ,  B01D35/027 ,  C12M1/12 ,  C12M1/26 ,  B01D29/13

Abstract: A method for separating microcarriers from a medium includes transferring a suspension that includes medium, microcarriers and cells into a compartment of a filter assembly. The filter assembly includes: a container having an interior surface bounding the compartment; and a filter having a tubular stem with an encircling sidewall extending between a first end and an opposing second end, the first end being secured to the interior surface of the container and the second end projecting into the compartment of the container, a plurality of openings extend through the sidewall of the stem that are large enough to allow the medium and cells to pass therethrough while preventing the microcarriers from passing therethrough. The medium and cells within the compartment are allowed to pass through the plurality of openings of the filter while the microcarriers are retained within the compartment of the container.

公开(公告)号：US10934514B2

公开(公告)日：2021-03-02

申请号：US16389688

申请日：2019-04-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeremy K. Larsen ,  Jon Q. Coleman ,  David S. Rowley

IPC: B01D29/27 ,  B01D35/027 ,  B01D29/13 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26

Abstract: A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.

公开(公告)号：US20190241855A1

公开(公告)日：2019-08-08

申请号：US16389688

申请日：2019-04-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeremy K. Larsen ,  Jon Q. Coleman ,  David S. Rowley

IPC: C12M1/00 ,  C12M1/12 ,  B01D29/27 ,  C12M1/26 ,  B01D29/13 ,  B01D35/027

CPC classification number: C12M29/04 ,  B01D29/13 ,  B01D29/27 ,  B01D35/027 ,  C12M23/14 ,  C12M23/26 ,  C12M25/16 ,  C12M33/14 ,  C12M47/02

Abstract: A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.