公开(公告)号：US20240263168A1

公开(公告)日：2024-08-08

申请号：US18636580

申请日：2024-04-16

Applicant: Life Technologies Corporation

Inventor： Chiu Tai Andrew Wong ,  Mark Beauchemin ,  Shanti Shankar ,  Kylan Szeto

IPC: C12N15/10 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/10

CPC classification number: C12N15/1065 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/1081

Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

公开(公告)号：US11959074B2

公开(公告)日：2024-04-16

申请号：US17525737

申请日：2021-11-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Kylan Szeto ,  Shanti Shankar ,  Mark Beauchemin

IPC: C12N15/10 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/10

CPC classification number: C12N15/1065 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/1081

Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.