公开(公告)号：US12050196B2

公开(公告)日：2024-07-30

申请号：US17499676

申请日：2021-10-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Todd Rearick ,  John Donohue

IPC: G01N27/414 ,  G16B30/00

CPC classification number: G01N27/4145 ,  G16B30/00

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

公开(公告)号：US11169111B2

公开(公告)日：2021-11-09

申请号：US16362367

申请日：2019-03-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Todd Rearick ,  John Donohue

IPC: G01N27/414 ,  G16B30/00

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

公开(公告)号：US10416112B2

公开(公告)日：2019-09-17

申请号：US14853026

申请日：2015-09-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Todd Rearick ,  John Donohue

IPC: C12Q1/68 ,  G06F19/22 ,  G01N27/414

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

公开(公告)号：US20240263168A1

公开(公告)日：2024-08-08

申请号：US18636580

申请日：2024-04-16

Applicant: Life Technologies Corporation

Inventor： Chiu Tai Andrew Wong ,  Mark Beauchemin ,  Shanti Shankar ,  Kylan Szeto

IPC: C12N15/10 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/10

CPC classification number: C12N15/1065 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/1081

Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

公开(公告)号：US11959074B2

公开(公告)日：2024-04-16

申请号：US17525737

申请日：2021-11-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Kylan Szeto ,  Shanti Shankar ,  Mark Beauchemin

IPC: C12N15/10 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/10

CPC classification number: C12N15/1065 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/1081

Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

公开(公告)号：US20240182965A1

公开(公告)日：2024-06-06

申请号：US18437474

申请日：2024-02-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Chiu Tai Andrew Wong ,  David Marran

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.

公开(公告)号：US11905558B2

公开(公告)日：2024-02-20

申请号：US16994318

申请日：2020-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Chiu Tai Andrew Wong ,  David Marran

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.