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    METHODS AND KITS FOR EXTRACTION OF DNA
    1.
    发明申请
    METHODS AND KITS FOR EXTRACTION OF DNA 审中-公开

    公开(公告)号：US20160326515A1

    公开(公告)日：2016-11-10

    申请号：US15216642

    申请日：2016-07-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Maxim BREVNOV ,  Hemant PAWAR ,  Manohar FURTADO ,  Jaiprakash SHEWALE

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2523/308

    Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

    METHODS AND KITS FOR EXTRACTION OF DNA
    2.
    发明申请
    METHODS AND KITS FOR EXTRACTION OF DNA 有权
    提取DNA的方法和工具

    公开(公告)号：US20140039176A1

    公开(公告)日：2014-02-06

    申请号：US13931110

    申请日：2013-06-28

    Applicant: Life Technologies Corporation

    Inventor： Maxim BREVNOV ,  Hemant PAWAR ,  Manohar FURTADO ,  Jaiprakash SHEWALE

    IPC: C12N15/10

    CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2523/308

    Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

    Abstract translation: 公开了用于从溶液中回收生物聚合物的方法和材料。 特别地，本发明提供从生物材料中提取和分离核酸的方法。 在洗涤剂和溶剂的存在下，可以通过与可溶性多糖聚合物和磁性颗粒形成稳定的络合物来分离核酸。 当颗粒从溶液中磁性分离出来时，核酸与它们分离。 随后可以将核酸从颗粒中释放并分离出来。 核酸制备物可用于实现下游分子技术的有效和准确的结果，例如定量，核酸来源的鉴定和基因分型。

    METHODS, WORKFLOWS, KITS, APPARATUSES, AND COMPUTER PROGRAM MEDIA FOR NUCLEIC ACID SAMPLE PREPARATION FOR NUCLEIC ACID SEQUENCING
    3.
    发明申请
    METHODS, WORKFLOWS, KITS, APPARATUSES, AND COMPUTER PROGRAM MEDIA FOR NUCLEIC ACID SAMPLE PREPARATION FOR NUCLEIC ACID SEQUENCING 审中-公开
    用于核酸序列的核酸样品制备的方法，工作流程，工具，设备和计算机程序介质

    公开(公告)号：US20150225774A1

    公开(公告)日：2015-08-13

    申请号：US14603076

    申请日：2015-01-22

    Applicant: Life Technologies Corporation

    Inventor： Maxim G. BREVNOV ,  Elena BOLCHAKOVA ,  Manohar FURTADO

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6869 ,  C12Q2563/131 ,  C12Q2563/143

    Abstract: A method for preparing a nucleic acid sample for nucleic acid sequencing includes amplifying a nucleic acid target sequence using a primer bound to a first capture substrate; capturing an amplified nucleic acid product by the first capture substrate; generating at least one sequencing ladder from the amplified nucleic acid product using at least one sequencing primer; capturing the at least one sequencing ladder by hybridizing the at least one sequencing ladder to a complementary capture compound on a second capture substrate; and removing the at least one sequencing ladder from the second capture substrate. The first and/or second capture substrate may include a magnetic particle. Other methods, workflows, kits, and computer program media for nucleic acid sample preparation are also disclosed.

    Abstract translation: 用于制备用于核酸测序的核酸样品的方法包括使用与第一捕获底物结合的引物扩增核酸靶序列; 通过第一捕获基质捕获扩增的核酸产物; 使用至少一个测序引物从扩增的核酸产物产生至少一个测序梯; 通过将所述至少一个测序梯度杂交到第二捕获基底上的互补捕获化合物来捕获所述至少一个测序梯; 以及从所述第二捕获基板去除所述至少一个测序梯。 第一和/或第二捕获基板可以包括磁性颗粒。 还公开了用于核酸样品制备的其它方法，工作流程，试剂盒和计算机程序培养基。

    ANALYSIS OF Y-CHROMOSOME STR MARKERS
    4.
    发明申请
    ANALYSIS OF Y-CHROMOSOME STR MARKERS 审中-公开

    公开(公告)号：US20180298453A1

    公开(公告)日：2018-10-18

    申请号：US15947750

    申请日：2018-04-06

    Applicant: Life Technologies Corporation

    Inventor： Rixun FANG ,  Manohar FURTADO ,  Manfred KAYSER ,  Kaye BALLANTYNE

    IPC: C12Q1/6888

    Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

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