公开(公告)号：US20150307869A1

公开(公告)日：2015-10-29

申请号：US14697962

申请日：2015-04-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kevin HACKER ,  Gregory GOVONI ,  Nikolay SERGEEV ,  Elena BOLCHAKOVA ,  Maxim BREVNOV ,  Manohar FURTADO ,  Johnie YOUNG ,  James NURSE ,  Mariela CUADRAS

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  B01D29/13 ,  C12N1/066 ,  C12Q1/68 ,  C12Q1/689 ,  C12Q1/6895 ,  G01N21/75

Abstract: Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.

Abstract translation: 提供了用于检测诸如饮用水，药物溶液和组织培养基的流体中的微生物污染物如细菌和真菌的装置和方法。 更具体地，提供了用于捕获和处理来自流体的微生物的过滤装置，以及基于物理破坏与小珠和裂解溶液的组合的微生物的回收，裂解和检测的改进方法。

公开(公告)号：US20160341640A1

公开(公告)日：2016-11-24

申请号：US15157011

申请日：2016-05-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim BREVNOV ,  James NURSE ,  James STRAY

IPC: G01N1/40

CPC classification number: G01N1/4005 ,  G01N2001/4016

Abstract: A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter.

Abstract translation: 用于样品处理的装置可以包括具有出口的至少一个室，所述室被配置为接收用于处理的样品，过滤器，所述至少一个室中的至少一些样品部分通过所述过滤器和设置在所述室中的阻挡构件 第一状态以在至少一个室中容纳样品。 在足够的条件下，阻挡构件可以改变到第二状态，以允许包含在腔室中的至少一些样品部分沿流动方向流向出口并通过过滤器。

公开(公告)号：US20160326515A1

公开(公告)日：2016-11-10

申请号：US15216642

申请日：2016-07-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim BREVNOV ,  Hemant PAWAR ,  Manohar FURTADO ,  Jaiprakash SHEWALE

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2523/308

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

公开(公告)号：US20140039176A1

公开(公告)日：2014-02-06

申请号：US13931110

申请日：2013-06-28

Applicant: Life Technologies Corporation

Inventor： Maxim BREVNOV ,  Hemant PAWAR ,  Manohar FURTADO ,  Jaiprakash SHEWALE

IPC: C12N15/10

CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2523/308

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

Abstract translation: 公开了用于从溶液中回收生物聚合物的方法和材料。 特别地，本发明提供从生物材料中提取和分离核酸的方法。 在洗涤剂和溶剂的存在下，可以通过与可溶性多糖聚合物和磁性颗粒形成稳定的络合物来分离核酸。 当颗粒从溶液中磁性分离出来时，核酸与它们分离。 随后可以将核酸从颗粒中释放并分离出来。 核酸制备物可用于实现下游分子技术的有效和准确的结果，例如定量，核酸来源的鉴定和基因分型。