公开(公告)号：US20230122742A1

公开(公告)日：2023-04-20

申请号：US17976128

申请日：2022-10-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. JANAWAY ,  Mark ANDERSEN ,  Kornelija ZGONC ,  Michael C. PALLAS ,  Marcin SIKORA ,  Casey R. McFARLAND ,  Ferrier N. LE ,  Haopeng Wang ,  Jian GONG ,  Gothami Padmabandu

IPC: C12Q1/6886 ,  C12Q1/686 ,  G01N21/64

Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

公开(公告)号：US20200224280A1

公开(公告)日：2020-07-16

申请号：US16741299

申请日：2020-01-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. JANAWAY ,  Mark ANDERSEN ,  Kornelija ZGONC ,  Michael C. PALLAS ,  Marcin SIKORA ,  Casey R. McFARLAND ,  Ferrier N. LE ,  Haopeng Wang ,  Jian GONG ,  Gothami Padmabandu

IPC: C12Q1/6886 ,  C12Q1/686 ,  G01N21/64

Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

公开(公告)号：US20230030218A1

公开(公告)日：2023-02-02

申请号：US17963135

申请日：2022-10-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eliodor GHENCIU ,  Theodore E. STRAUB ,  Evan W. FOSTER ,  Michael C. PALLAS

IPC: G01N35/10 ,  C40B60/14 ,  B01L3/00 ,  B01J19/00

Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

公开(公告)号：US20200319221A1

公开(公告)日：2020-10-08

申请号：US16852830

申请日：2020-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eliodor GHENCIU ,  Theodore E. STRAUB ,  Evan W. FOSTER ,  Michael C. PALLAS

IPC: G01N35/10 ,  C40B60/14 ,  B01L3/00 ,  B01J19/00

Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

公开(公告)号：US20200241023A1

公开(公告)日：2020-07-30

申请号：US16777820

申请日：2020-01-30

Applicant: Life Technologies Holdings PTE Limited ,  Life Technologies Corporation

Inventor： Kuan Moon BOO ,  Wei Fuh TEO ,  Zeqi TAN ,  Soo Yong LAU ,  Ming Tiong SIA ,  Woon Liang SOH ,  Ching Yee LAM ,  Shan Hua DONG ,  Marc HABERSTROH ,  Michael C. PALLAS ,  Hardeep Sangha

IPC: G01N35/00 ,  B01L3/00 ,  B01L7/00 ,  G06K7/10 ,  G06K19/07

Abstract: A system for biological analysis includes a housing, a block assembly within the housing having a sample block and a baseplate, a heated cover and a cover carrier. The sample block receives a sample holder comprising an RFID tag. A first drive mechanism generates relative movement between the sample block and the baseplate along a first axis. A second drive mechanism generates relative movement between the heated cover and the cover carrier along a second axis that is different from the first axis. Based on a first command the first drive mechanism releasably engages the sample block and operates the second drive mechanism to releasably engage the heated cover with the cover carrier. The system also includes first and second RFID antennas that receive RFID data from the sample holder RFID tag that is read by at least one RFID reader.