公开(公告)号：US20180208996A1

公开(公告)日：2018-07-26

申请号：US15923954

申请日：2018-03-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6886 ,  C12Q1/6858

CPC classification number: C12Q1/6886 ,  C07H21/04 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20200347447A1

公开(公告)日：2020-11-05

申请号：US15930101

申请日：2020-05-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6858 ,  C12Q1/6883 ,  C12Q1/686

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20240132946A1

公开(公告)日：2024-04-25

申请号：US18087259

申请日：2022-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20200255907A1

公开(公告)日：2020-08-13

申请号：US16745296

申请日：2020-01-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6886 ,  C12Q1/6858

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20230323472A1

公开(公告)日：2023-10-12

申请号：US18075729

申请日：2022-12-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6886 ,  C12Q1/6858

CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C07H21/04

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20160076090A1

公开(公告)日：2016-03-17

申请号：US14856448

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20180355420A1

公开(公告)日：2018-12-13

申请号：US16109191

申请日：2018-08-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).