公开(公告)号：US20180208996A1

公开(公告)日：2018-07-26

申请号：US15923954

申请日：2018-03-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6886 ,  C12Q1/6858

CPC classification number: C12Q1/6886 ,  C07H21/04 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20240132946A1

公开(公告)日：2024-04-25

申请号：US18087259

申请日：2022-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20200255907A1

公开(公告)日：2020-08-13

申请号：US16745296

申请日：2020-01-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6886 ,  C12Q1/6858

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20180355420A1

公开(公告)日：2018-12-13

申请号：US16109191

申请日：2018-08-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20220195511A1

公开(公告)日：2022-06-23

申请号：US17574032

申请日：2022-01-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kelly LI ,  Shoulian DONG ,  Caifu CHEN

IPC: C12Q1/6858

Abstract: The present disclosure is drawn to methods for detection, quantitation and analysis of nucleotides of interest, for example SNPs, in nucleic acid sequences of interest using universal FRET-based reporter primers.

公开(公告)号：US20200347447A1

公开(公告)日：2020-11-05

申请号：US15930101

申请日：2020-05-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6858 ,  C12Q1/6883 ,  C12Q1/686

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20180171400A1

公开(公告)日：2018-06-21

申请号：US15887159

申请日：2018-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda WONG ,  Caifu CHEN ,  Yalei WU ,  Shoulian DONG ,  Chunmei LIU

IPC: C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6858 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6832 ,  C12Q1/6848

CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

公开(公告)号：US20150140557A1

公开(公告)日：2015-05-21

申请号：US14445046

申请日：2014-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying Tan

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

Abstract translation: 在一些实施方案中，本发明一般涉及用于区分不同等位基因之间的序列变异的组合物，方法和试剂盒。 更具体地说，在一些实施方案中，本发明提供了用于定量稀少（例如，突变体）等位基因变体如SNP或核苷酸（NT）插入或缺失的组合物，方法和试剂盒，其包含丰富的（例如，野生型 ）等位基因变体，具有高特异性和选择性。 特别地，在一些实施方案中，本发明涉及用于突变检测的高选择性方法，称为竞争性等位基因特异性TaqMan PCR（“cast-PCR”）。

公开(公告)号：US20230323472A1

公开(公告)日：2023-10-12

申请号：US18075729

申请日：2022-12-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6886 ,  C12Q1/6858

CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C07H21/04

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20220119875A1

公开(公告)日：2022-04-21

申请号：US17562346

申请日：2021-12-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda WONG ,  Caifu CHEN ,  Yalei WU ,  Shoulian DONG ,  Chunmei LIU

IPC: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853

Abstract: Methods, compositions, and kits for capturing, detecting, and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.