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    Probe:antiprobe compositions for high specificity DNA or RNA detection
    2.
    发明授权
    Probe:antiprobe compositions for high specificity DNA or RNA detection 有权

    公开(公告)号：US11667971B2

    公开(公告)日：2023-06-06

    申请号：US14216413

    申请日：2014-03-17

    Applicant: David A. Shafer

    Inventor： David A. Shafer

    IPC: C07H21/02 ,  C12Q1/6876 ,  C12Q1/6832

    CPC classification number: C12Q1/6876 ,  C12Q1/6832 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2537/163

    Abstract: Probe systems and methods are provided for detecting nucleic acid targets using labeled polynucleotide probes and antiprobes that interact together and with complementary targets. These interactions result in signaling changes that indicate target frequency and provide error-checking functions that facilitate single base discrimination. These probe:antiprobe compositions enable real-time PCR detection, end-point detection and microarray detection of microbial species, drug resistant mutants, and cancer related variants. The probe:antiprobe may be an internal probe between two primers or may be a primer-probe. The probe also may be modified by introducing a base mismatch to increase thermodynamic discrimination of a correct versus incorrect target differing by a single base. Probe systems also are provided for use in methods of increasing target amplification and detecting specific single base variants.

    Rapid, highly-sensitive, and highly-specific nucleic acid detection
    6.
    发明授权
    Rapid, highly-sensitive, and highly-specific nucleic acid detection 有权

    公开(公告)号：US09851330B2

    公开(公告)日：2017-12-26

    申请号：US15063355

    申请日：2016-03-07

    Applicant: KONICA MINOLTA LABORATORY U.S.A., INC.

    Inventor： Noriaki Yamamoto

    IPC: G01N27/447 ,  C12Q1/68

    CPC classification number: G01N27/44791 ,  C12Q1/6827 ,  G01N27/447 ,  G01N27/44721 ,  C12Q2537/161 ,  C12Q2563/107 ,  C12Q2565/125 ,  C12Q2565/629 ,  C12Q2563/125

    Abstract: A nucleic acid (NA) detection method combines ultra-specific probe, on-chip isotachophoresis (ITP) which can separate single strand and double strand NAs, and enzyme amplification. The ITP device has a sieving matrix between the LE (leading electrolyte) and TE (trailing electrolyte) reservoirs, for separating double-strand and single-strand NAs. The LE or TE reservoir also contains a spacer ion having a mobility between the LE and the TE. The sample and a double-strand NA probe is added to the TE reservoir, the probe being formed of a protector strand modified with a fluorescent molecule and a complement strand, where the protector strand is released in the presence of the target NA. Fluorescent signal is detected downstream of the sieving matrix. Alternatively, the protector strand is modified with an enzyme and a single-strand NA modified with a substrate of the enzyme is added to the reaction mixture downstream of the sieving matrix.

    Method of distinguishing genotypes
    8.
    发明授权
    Method of distinguishing genotypes 有权
    区分基因型的方法

    公开(公告)号：US09523119B2

    公开(公告)日：2016-12-20

    申请号：US13248321

    申请日：2011-09-29

    Applicant: Akio Yamane ,  Mashimo Nakayama ,  Shiro Kitano

    Inventor： Akio Yamane ,  Mashimo Nakayama ,  Shiro Kitano

    IPC: C12Q1/68 ,  G01N21/64

    CPC classification number: C12Q1/686 ,  C12Q1/6858 ,  G01N2021/6441 ,  C12Q2537/161 ,  C12Q2535/131 ,  C12Q2531/119 ,  C12Q2525/186 ,  C12Q2527/125

    Abstract: The present invention relates to a method of distinguishing genotypes using PCR-PHFA including: a nucleic acid amplification step in which a mutation site-including region of a gene is amplified by a nucleic acid amplification reaction, thereby obtaining an amplification reaction solution; and a distinction step in which the amplification reaction solution obtained from the nucleic acid amplification step is mixed with a reference double-stranded nucleic acid having a specific genotype on the mutation site as well as being labeled with a labeling substance, and the mixture is subjected to a competitive strand displacement reaction, and the level of the occurrence of strand displacement is assessed so as to distinguish the identity; and the competitive strand displacement reaction is performed under a condition to suppress a polymerase extension reaction, and a genotype distinguishing kit for use in the distinct of genotypes by this method.

    Abstract translation: 本发明涉及使用PCR-PHFA区分基因型的方法，包括：核酸扩增步骤，其中通过核酸扩增反应扩增基因的突变位点包含区域，从而获得扩增反应溶液; 以及将从核酸扩增步骤得到的扩增反应溶液与突变位点上具有特定基因型的参照双链核酸混合并用标记物质标记的鉴别步骤， 进行竞争性股份置换反应，评估股份置换的发生水平，以区分身份; 并且在抑制聚合酶延伸反应的条件下进行竞争性链置换反应，以及通过该方法在不同基因型中使用的基因型鉴别试剂盒。

    Compositions and methods for the identification of inhibitors of retroviral infection
    9.
    发明授权
    Compositions and methods for the identification of inhibitors of retroviral infection 有权
    用于鉴定逆转录病毒感染抑制剂的组合物和方法

    公开(公告)号：US09249473B2

    公开(公告)日：2016-02-02

    申请号：US13916335

    申请日：2013-06-12

    Applicant: Trana Discovery, Inc.

    Inventor： Richard H. Guenther

    IPC: C12Q1/68 ,  C12Q1/70 ,  C12N9/99

    CPC classification number: C12Q1/703 ,  C12N9/99 ,  C12N2740/10011 ,  C12N2740/16011 ,  C12Q2600/136 ,  C12Q2537/161 ,  C12Q2527/127 ,  C12Q2521/107

    Abstract: Methods of identifying inhibitors of retroviral propagation, tRNA used in the methods, and kits, including the tRNA, which can be used in the methods, are disclosed. Methods of treating or preventing retroviral infections by administering an effective amount of the inhibitors, and pharmaceutical compositions including the inhibitors, are also disclosed. The methods involve forming a mixture comprising a linear sequence of a tRNA anticodon stem loop fragment that is not capable of forming a stem-loop, a target nucleic acid molecule capable of binding to the tRNA anticodon stem loop fragment, and a test compound. The mixture is incubated under conditions that allow binding of the tRNA anticodon stem loop fragment and the target nucleic acid molecule in the absence of the test compound. Assays can then be performed that detect whether or not the test compound inhibits the binding of the tRNA anticodon stem loop fragment and the target nucleic acid molecule.

    Abstract translation: 公开了确定逆转录病毒繁殖抑制剂的方法，方法中使用的tRNA和可用于该方法的试剂盒，包括tRNA。 还公开了通过施用有效量的抑制剂治疗或预防逆转录病毒感染的方法，以及包括抑制剂的药物组合物。 所述方法包括形成包含不能形成茎环的tRNA反密码子茎环片段的线性序列的混合物，能够结合tRNA反密码子茎环片段的靶核酸分子和测试化合物。 在不存在测试化合物的情况下，将该混合物在允许tRNA反密码子茎环片段和靶核酸分子结合的条件下孵育。 然后可以进行检测，以检测测试化合物是否抑制tRNA反密码子茎环片段和靶核酸分子的结合。

    METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING
    10.
    发明申请
    METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING 审中-公开
    非侵入式预防性呼吸方法

    公开(公告)号：US20150072872A1

    公开(公告)日：2015-03-12

    申请号：US14546321

    申请日：2014-11-18

    Applicant: Natera, Inc.

    Inventor： Matthew Rabinowitz ,  George Gemelos ,  Milena Banjevic ,  Allison Ryan ,  Zachary Demko ,  Matthew Hill ,  Bernhard Zimmermann ,  Johan Baner

    IPC: C12Q1/68 ,  G06F19/00 ,  G06F19/18

    CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q2537/161 ,  C12Q2537/165 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172 ,  G06F19/34 ,  G16B5/00 ,  G16B20/00 ,  G16B30/00 ,  G16H50/30

    Abstract: The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a sample of DNA from the mother of the fetus and from the fetus, and from genotypic data from the mother and optionally also from the father. The ploidy state is determined by using a joint distribution model to create a set of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. In an embodiment, the mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias.

    Abstract translation: 本公开提供了用于从胎儿母体和胎儿的DNA样品测量的基因型数据以及来自母亲的基因型数据以及任选地还可以从父亲的基因型数据确定胎儿胎儿中染色体的倍性状态的方法 。 通过使用联合分布模型来确定给定父母基因型数据的不同可能胎儿倍性状态的一组预期等位基因分布，并将预期等位基因分布与在混合样品中测量的测量等位基因分布的模式进行比较来确定倍性状态， 并选择其预期等位基因分布模式与观察到的等位基因分布模式最接近的倍性状态。 在一个实施方案中，DNA的混合样品可以以使等位基因偏倚最小化的方式优先富集在多个多态位点。

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