公开(公告)号：US20250092261A1

公开(公告)日：2025-03-20

申请号：US18580853

申请日：2022-07-21

Applicant: Life Technologies Corporation

Inventor： Andrew Levitz ,  Scott Benson ,  Brian Evans ,  Khairuzzaman Bashar Mullah

IPC: C09B57/14 ,  C12Q1/686 ,  G01N21/64

Abstract: The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described. Also disclosed is a method of detecting or quantifying a target nucleic acid molecule in a sample by polymerase chain reaction (PCR). The compounds have the following formula (I).

公开(公告)号：US11773426B2

公开(公告)日：2023-10-03

申请号：US16514814

申请日：2019-07-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Joun ,  Chieh-Yuan Li ,  Brian Reed ,  Craig Obergfell ,  Devin Dressman ,  Abraham Rosenbaum ,  Scott Benson ,  Andi Broka ,  Srinka Ghosh

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6844

CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q1/6844 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537

Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

公开(公告)号：US09080968B2

公开(公告)日：2015-07-14

申请号：US13734696

申请日：2013-01-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christina Inman ,  Alexander Mastroianni ,  Wolfgang Hinz ,  Shifeng Li ,  Scott Benson

IPC: H01L27/14 ,  G01N27/414

CPC classification number: H01L21/31105 ,  G01N27/4145 ,  H01L21/31144

Abstract: A method of manufacturing a sensor, the method including forming an array of chemically-sensitive field effect transistors (chemFETs), depositing a dielectric layer over the chemFETs in the array, depositing a protective layer over the dielectric layer, etching the dielectric layer and the protective layer to form cavities corresponding to sensing surfaces of the chemFETs, and removing the protective layer. The method further includes, etching the dielectric layer and the protective layer together to form cavities corresponding to sensing surfaces of the chemFETs. The protective layer is at least one of a polymer, photoresist material, noble metal, copper oxide, and zinc oxide. The protective layer is removed using at least one of sodium hydroxide, organic solvent, aqua regia, ammonium carbonate, hydrochloric acid, acetic acid, and phosphoric acid.

Abstract translation: 一种制造传感器的方法，所述方法包括形成化学敏感场效应晶体管阵列（chemFET），在阵列中的chemFET上沉积介电层，在电介质层上沉积保护层，蚀刻电介质层和 保护层以形成对应于chemFET的感测表面的空腔，以及去除保护层。 该方法还包括：将电介质层和保护层一起蚀刻以形成对应于chemFET的感测表面的空腔。 保护层是聚合物，光致抗蚀剂材料，贵金属，氧化铜和氧化锌中的至少一种。 使用氢氧化钠，有机溶剂，王水，碳酸铵，盐酸，乙酸和磷酸中的至少一种除去保护层。