公开(公告)号：US09879243B2

公开(公告)日：2018-01-30

申请号：US13968157

申请日：2013-08-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Valentina C. Ciccarone ,  Dale Gruber ,  Shelly Bennett

IPC: C12N15/63 ,  C12N15/11 ,  C12N15/113 ,  C12N9/38 ,  C12N15/88 ,  C12N5/00

CPC classification number: C12N9/2471 ,  C12N5/0018 ,  C12N5/0043 ,  C12N5/005 ,  C12N15/88 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2501/999 ,  C12N2510/02

Abstract: The present invention is directed generally to cell culture media useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells) in the presence of said media. Cells containing introduced materials can be further cultured in the media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention thus provides efficient and high throughput methods to transform/transfect culture and cells avoiding the need for multiple manipulations and transfers of cells during transfection and expression studies.

公开(公告)号：US20140057335A1

公开(公告)日：2014-02-27

申请号：US13968157

申请日：2013-08-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Valentina C. CICCARONE ,  Dale Gruber ,  Shelly Bennett

IPC: C12N9/38 ,  C12N5/00

CPC classification number: C12N9/2471 ,  C12N5/0018 ,  C12N5/0043 ,  C12N5/005 ,  C12N15/88 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2501/999 ,  C12N2510/02

Abstract: The present invention is directed generally to cell culture media useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells in the presence of said media. Cells containing introduced materials can be further cultured in the media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention thus provides efficient and high throughput methods to transform/transfect culture and cells avoiding the need for multiple manipulations and transfers of cells during transfection and expression studies.

Abstract translation: 本发明一般涉及用于将大分子和化合物（例如，核酸分子）引入细胞的细胞培养基（例如，在所述培养基存在下的真核细胞），含有引入的物质的细胞可以在培养基中进一步培养 特别地，本发明允许将核酸分子（例如，载体）引入细胞（特别是真核细胞）中，以及由细胞中的核酸分子编码的蛋白质的表达。本发明无需每次更换细胞培养基 因此，本发明提供了有效和高通量的方法来转化/转染培养物和细胞，避免了在转染和表达研究期间对细胞进行多重操作和转移的需要。