公开(公告)号：US20210324390A1

公开(公告)日：2021-10-21

申请号：US17259699

申请日：2019-07-12

申请人： LONZA LTD

发明人： Robert YOUNG ,  Peter Michael O'CALLAGHAN ,  David JAMES ,  Stephen JAFFE

IPC分类号： C12N15/65 ,  C12N15/11 ,  C12N9/22

摘要： The present disclosure features methods, cells or cell lines, and compositions for increasing the amount of products, e.g., proteins, produced by a cell or cell line by reducing the level of a non-essential endogenous protein.

公开(公告)号：US20230047838A1

公开(公告)日：2023-02-16

申请号：US17815465

申请日：2022-07-27

申请人： LONZA LTD

发明人： James BUDGE ,  Christopher Mark SMALES ,  Tanya Jeane KNIGHT ,  Robert YOUNG

IPC分类号： C12N9/02 ,  C12P21/02 ,  C07K14/47 ,  C07K16/00 ,  C12N5/071

摘要： The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.

公开(公告)号：US20200208171A1

公开(公告)日：2020-07-02

申请号：US16622509

申请日：2018-06-15

申请人： LONZA LTD

发明人： Robert YOUNG ,  Peter Michael O'CALLAGHAN ,  Thomas PAYNE

IPC分类号： C12N15/85 ,  C12N15/11 ,  C12N9/22 ,  C12P21/00

摘要： Disclosed are genetic control circuits, cells, and methods that use a repressor polypeptide to reduce the transcription rate of an exogenous therapeutic polypeptide encoding gene in response to a change in condition.