公开(公告)号：US20170145462A1

公开(公告)日：2017-05-25

申请号：US15418280

申请日：2017-01-27

Applicant: Novozymes, Inc.

Inventor： Jeffrey Shasky ,  Wendy Yoder

IPC: C12P21/00 ,  C12N9/88 ,  C12N9/58 ,  C12N15/80 ,  C12N9/30

CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

公开(公告)号：US09790532B2

公开(公告)日：2017-10-17

申请号：US15418280

申请日：2017-01-27

Applicant: Novozymes, Inc.

Inventor： Jeffrey Shasky ,  Wendy Yoder

IPC: C12P21/00 ,  C12N15/80 ,  C12N9/30 ,  C12N9/58 ,  C12N9/88

CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

公开(公告)号：US09255275B2

公开(公告)日：2016-02-09

申请号：US14168766

申请日：2014-01-30

Applicant: Novozymes, Inc.

Inventor： Jeffrey Shasky ,  Wendy Yoder

IPC: C12N15/80 ,  C12N9/30 ,  C12N9/58 ,  C12N9/88 ,  C12P21/02 ,  C12P21/00

CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

Abstract translation: 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。

公开(公告)号：US09593342B2

公开(公告)日：2017-03-14

申请号：US14974879

申请日：2015-12-18

Applicant: Novozymes, Inc.

Inventor： Jeffrey Shasky ,  Wendy Yoder

IPC: C12N15/80 ,  C12N9/30 ,  C12N9/58 ,  C12N9/88 ,  C12P21/02 ,  C12P21/00

CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

Abstract translation: 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。

公开(公告)号：US20160102315A1

公开(公告)日：2016-04-14

申请号：US14974879

申请日：2015-12-18

Applicant: NOVOZYMES, INC.

Inventor： Jeffrey Shasky ,  Wendy Yoder

IPC: C12N15/80 ,  C12P21/00

CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

Abstract translation: 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。

公开(公告)号：US20140147847A1

公开(公告)日：2014-05-29

申请号：US14168766

申请日：2014-01-30

Applicant: Novozymes, Inc.

Inventor： Jeffrey Shasky ,  Wendy Yoder

IPC: C12N15/80 ,  C12P21/00

CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

Abstract translation: 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。