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    Methods for producing polypeptides in protease-deficient mutants of Trichoderma
    1.
    发明授权
    Methods for producing polypeptides in protease-deficient mutants of Trichoderma 有权

    公开(公告)号：US11427847B2

    公开(公告)日：2022-08-30

    申请号：US17163033

    申请日：2021-01-29

    Applicant: Novozymes, Inc.

    Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

    IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N15/04 ,  C12N9/58

    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

    Methods for producing polypeptides in protease-deficient mutants of trichoderma
    2.
    发明授权
    Methods for producing polypeptides in protease-deficient mutants of trichoderma 有权

    公开(公告)号：US11078508B2

    公开(公告)日：2021-08-03

    申请号：US16211632

    申请日：2018-12-06

    Applicant: Novozymes, Inc.

    Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

    IPC: C12P21/00 ,  C12P21/02 ,  C12N9/58 ,  C12N15/80 ,  C12N15/04

    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

    Methods for producing polypeptides in enzyme-deficient mutants of fusarium venentatum
    4.
    发明申请
    Methods for producing polypeptides in enzyme-deficient mutants of fusarium venentatum 审中-公开

    公开(公告)号：US20170145462A1

    公开(公告)日：2017-05-25

    申请号：US15418280

    申请日：2017-01-27

    Applicant: Novozymes, Inc.

    Inventor： Jeffrey Shasky ,  Wendy Yoder

    IPC: C12P21/00 ,  C12N9/88 ,  C12N9/58 ,  C12N15/80 ,  C12N9/30

    CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

    Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

    Methods for Producing Polypeptides in Protease-Deficient Mutants of Trichoderma
    6.
    发明申请
    Methods for Producing Polypeptides in Protease-Deficient Mutants of Trichoderma 有权

    公开(公告)号：US20210155967A1

    公开(公告)日：2021-05-27

    申请号：US17163033

    申请日：2021-01-29

    Applicant: Novozymes, Inc.

    Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

    IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

    Methods for producing polypeptides in enzyme-deficient mutants of Fusarium venentatum
    7.
    发明授权
    Methods for producing polypeptides in enzyme-deficient mutants of Fusarium venentatum 有权
    在镰孢镰刀菌的缺酶突变体中产生多肽的方法

    公开(公告)号：US09593342B2

    公开(公告)日：2017-03-14

    申请号：US14974879

    申请日：2015-12-18

    Applicant: Novozymes, Inc.

    Inventor： Jeffrey Shasky ,  Wendy Yoder

    IPC: C12N15/80 ,  C12N9/30 ,  C12N9/58 ,  C12N9/88 ,  C12P21/02 ,  C12P21/00

    CPC classification number: C12P21/00 ,  C12N9/242 ,  C12N9/58 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01001 ,  C12Y401/01023 ,  Y02P20/52

    Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

    Abstract translation: 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。

    Methods for producing polypeptides in protease-deficient mutants of Trichoderma
    8.
    发明授权
    Methods for producing polypeptides in protease-deficient mutants of Trichoderma 有权

    公开(公告)号：US11078510B2

    公开(公告)日：2021-08-03

    申请号：US16996168

    申请日：2020-08-18

    Applicant: Novozymes, Inc.

    Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

    IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N15/04 ,  C12N9/58

    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

    Protease-deficient mutants of trichoderma for producing polypeptides
    9.
    发明授权
    Protease-deficient mutants of trichoderma for producing polypeptides 有权

    公开(公告)号：US10400260B2

    公开(公告)日：2019-09-03

    申请号：US14663118

    申请日：2015-03-19

    Applicant: Novozymes, Inc.

    Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

    IPC: C12N9/58 ,  C12P21/00 ,  C12N15/80 ,  C12P21/02

    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

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