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公开(公告)号：US20170145462A1
公开(公告)日：2017-05-25
申请号：US15418280
申请日：2017-01-27
申请人： Novozymes, Inc.
发明人： Jeffrey Shasky , Wendy Yoder
CPC分类号： C12P21/00 , C12N9/242 , C12N9/58 , C12N9/88 , C12N15/80 , C12P21/02 , C12Y302/01001 , C12Y401/01023 , Y02P20/52
摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.
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2.发明授权Methods for producing polypeptides in enzyme-deficient mutants of Fusarium venenatum 失效在镰孢镰刀菌酶缺陷突变体中产生多肽的方法公开(公告)号：US08647856B2
公开(公告)日：2014-02-11
申请号：US13121254
申请日：2009-09-30
申请人： Jeffrey Shasky , Wendy Yoder
发明人： Jeffrey Shasky , Wendy Yoder
CPC分类号： C12P21/00 , C12N9/242 , C12N9/58 , C12N9/88 , C12N15/80 , C12P21/02 , C12Y302/01001 , C12Y401/01023 , Y02P20/52
摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.
摘要翻译： 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。
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3.发明申请Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum 失效在镰孢镰刀菌酶缺陷突变体中产生多肽的方法公开(公告)号：US20110236928A1
公开(公告)日：2011-09-29
申请号：US13121254
申请日：2009-09-30
申请人： Jeffrey Shasky , Wendy Yoder
发明人： Jeffrey Shasky , Wendy Yoder
CPC分类号： C12P21/00 , C12N9/242 , C12N9/58 , C12N9/88 , C12N15/80 , C12P21/02 , C12Y302/01001 , C12Y401/01023 , Y02P20/52
摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.
摘要翻译： 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。
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4.发明申请Methods for producing polypeptides in enzyme-deficient mutants of fusarium venentatum 审中-公开在镰孢镰刀菌缺乏酶突变体中产生多肽的方法公开(公告)号：US20160102315A1
公开(公告)日：2016-04-14
申请号：US14974879
申请日：2015-12-18
申请人： NOVOZYMES, INC.
发明人： Jeffrey Shasky , Wendy Yoder
CPC分类号： C12P21/00 , C12N9/242 , C12N9/58 , C12N9/88 , C12N15/80 , C12P21/02 , C12Y302/01001 , C12Y401/01023 , Y02P20/52
摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.
摘要翻译： 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。
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公开(公告)号：US20140206084A1
公开(公告)日：2014-07-24
申请号：US14123351
申请日：2012-06-01
CPC分类号： C12N15/902 , C12N9/88 , C12N15/74 , C12P7/065 , C12Y401/01023 , Y02E50/17
摘要： The present invention provides a primer set used for transformation that imparts a uracil requiring property by deleting or destroying a gene coding for orotidine-5-phosphate decarboxylase in Moorella bacteria. The present invention is accomplished by a primer set that is used for creating a uracil requiring strain obtained by deleting or destroying a gene coding for orotidine-5-phosphate decarboxylase in Moorella bacteria by homologous recombination and is represented by SEQ ID No. 1 and 2 that amplify an upstream region adjacent to said gene coding for orotidine-5-phosphate decarboxylase.
摘要翻译： 本发明提供了一种用于转化的引物组，其通过在Moorella细菌中缺失或破坏编码乳清苷-5-磷酸脱羧酶的基因而赋予尿嘧啶需要性质。 本发明通过引物组来实现，该引物组用于通过同源重组来产生通过在Moorella细菌中缺失或破坏编码乳清细胞中的乳清苷-5-磷酸脱羧酶的基因获得的尿嘧啶，并且由SEQ ID No.1和2 其扩增与编码乳清苷-5-磷酸脱羧酶的所述基因相邻的上游区域。
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6.发明申请Methods for producing polypeptides in enzyme-deficient mutants of fusarium venentatum 审中-公开在镰孢镰刀菌缺乏酶突变体中产生多肽的方法公开(公告)号：US20140147847A1
公开(公告)日：2014-05-29
申请号：US14168766
申请日：2014-01-30
申请人： Novozymes, Inc.
发明人： Jeffrey Shasky , Wendy Yoder
CPC分类号： C12P21/00 , C12N9/242 , C12N9/58 , C12N9/88 , C12N15/80 , C12P21/02 , C12Y302/01001 , C12Y401/01023 , Y02P20/52
摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.
摘要翻译： 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。
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公开(公告)号：US20170349920A1
公开(公告)日：2017-12-07
申请号：US15320550
申请日：2015-06-22
发明人： Jung Hoon Sohn , Hyun Joo Park , Sun Hee Lee , Jung Hoon Bae , Bong Hyun Sung
CPC分类号： C12P7/56 , C12N1/16 , C12N9/0006 , C12N9/88 , C12P7/06 , C12R1/84 , C12Y101/01027 , C12Y401/01001 , C12Y401/01023 , Y02E50/17
摘要： The present invention relates to: a novel Pichia kudriavzevii microorganism NG7 showing heat resistance and acid resistance; a composition, for producing organic acid or alcohol, which comprises the microorganism and a culture of the same; and a method, for producing an organic acid or alcohol, which comprises culturing the microorganism.
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公开(公告)号：US09790532B2
公开(公告)日：2017-10-17
申请号：US15418280
申请日：2017-01-27
申请人： Novozymes, Inc.
发明人： Jeffrey Shasky , Wendy Yoder
CPC分类号： C12P21/00 , C12N9/242 , C12N9/58 , C12N9/88 , C12N15/80 , C12P21/02 , C12Y302/01001 , C12Y401/01023 , Y02P20/52
摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.
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9.发明授权Methods for producing polypeptides in enzyme-deficient mutants of Fusarium venenatum 有权在镰孢镰刀菌酶缺陷突变体中产生多肽的方法公开(公告)号：US09255275B2
公开(公告)日：2016-02-09
申请号：US14168766
申请日：2014-01-30
申请人： Novozymes, Inc.
发明人： Jeffrey Shasky , Wendy Yoder
CPC分类号： C12P21/00 , C12N9/242 , C12N9/58 , C12N9/88 , C12N15/80 , C12P21/02 , C12Y302/01001 , C12Y401/01023 , Y02P20/52
摘要： The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.
摘要翻译： 本发明涉及产生多肽的方法，其包括：（a）在用于产生多肽的培养基中培养亲本尖叶镰刀菌菌株的突变体，其中所述突变株包含编码多肽的多核苷酸和一个或多个（ 几种）选自pyrG，amyA和alpA的基因，其中所述一个或多个（若干）基因被修饰，使所述突变株缺乏产生选自下组的一种或多种（几种） -5'-单磷酸脱羧酶，α-淀粉酶和碱性蛋白酶，与相同条件下栽培的亲本镰刀菌镰孢菌株相比; 和（b）从培养基中回收多肽。 本发明还涉及镰刀菌菌株的酶缺陷型突变体及其生产方法。
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10.发明授权公开(公告)号：US06927026B1
公开(公告)日：2005-08-09
申请号：US09582779
申请日：1998-12-18
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/88 , C12N15/60 , C12Q1/68 , C07H21/04 , C07K14/37 , C12N15/80
CPC分类号： C12N9/88 , C12Y401/01023
摘要： The invention relates to an orotidine-5′-phosphate decarboxylase gene having the sequence of SEQ ID NO: 1 or its homologs, to a gene construct comprising this gene or its homologs, and to its use. The invention additionally relates to vectors or organisms comprising an orotidine-5′-phosphate decarboxylases gene having the sequence SEQ ID NO: 1 or its homologs. The invention further relates to a process for producing uracil-auxotrophic microorganisms and to a process for inserting DNA into uracil-auxotrophic microorganisms.
摘要翻译： 本发明涉及具有SEQ ID NO：1或其同系物序列的含有该基因或其同系物的基因构建体的乳清苷-5'-磷酸脱羧酶基因及其用途。 本发明还涉及包含具有序列SEQ ID NO：1或其同系物的乳清苷-5'-磷酸脱羧酶基因的载体或生物体。 本发明还涉及生产尿嘧啶 - 营养缺陷型微生物的方法和将DNA插入尿嘧啶 - 营养缺陷型微生物的方法。
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