公开(公告)号：US20190128888A1

公开(公告)日：2019-05-02

申请号：US16095340

申请日：2017-04-21

Applicant: OSAKA UNIVERSITY ,  TOKYO INSTITUTE OF TECHNOLOGY ,  National University Corporation Nagoya University ,  NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY

Inventor： Makusu TSUTSUI ,  Kazumichi YOKOTA ,  Masateru TANIGUCHI ,  Tomoji KAWAI ,  Mina OKOCHI ,  Masayoshi TANAKA ,  Yoshinobu BABA ,  Noritada KAJI ,  Takao YASUI ,  Yuji MIYAHARA ,  Yukichi HORIGUCHI

IPC: G01N33/569 ,  G01N33/566 ,  C12Q1/04 ,  C12Q1/68 ,  C07K7/08 ,  C07K7/06 ,  C12M1/26

Abstract: A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.

公开(公告)号：US20200251184A1

公开(公告)日：2020-08-06

申请号：US16470140

申请日：2017-12-12

Applicant: Osaka University

Inventor： Takashi WASHIO ,  Tomoji KAWAI ,  Masateru TANIGUCHI ,  Makusu TSUTSUI ,  Kazumichi YOKOTA ,  Akira ISHI ,  Takeshi YOSHIDA

IPC: G16B40/10 ,  G06K9/62 ,  G06N20/10 ,  G06F17/18 ,  G01N27/327 ,  G01N33/487

Abstract: The present invention provides a classification analysis method, a classification analysis device, and a storage medium for classification analysis, which enable, with high accuracy, the classification analysis of particulate or molecular analytes. As a means for solving the problem, a data group of particle-passage detection signals is based which are detected by a nanopore device 8 in accordance with passage of subject particles through a through-hole 12. A feature value is obtained in advance which indicates the feature of the waveform of the pulse signals corresponding to the passage of the predetermined analyte and the feature value obtained in advance is set as the learning data for the machine learning. The feature value obtained from the pulse signals of said analyzed data is set as a variable and the classification analysis on the predetermined analytes in the analyzed data can be performed by executing a classification analysis program due to the machine learning.

公开(公告)号：US20190257787A1

公开(公告)日：2019-08-22

申请号：US16065633

申请日：2016-12-19

Applicant: Osaka University

Inventor： Takashi WASHIO ,  Tomoji KAWAI ,  Masateru TANIGUCHI ,  Makusu TSUTSUI ,  Kazumichi YOKOTA ,  Akira ISHI ,  Takeshi YOSHIDA

IPC: G01N27/447 ,  G01N33/487

Abstract: The present invention provides a number analyzing method, a number analyzing device, and a storage medium for number analysis, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device (8) in accordance with passage of subject particles through a through-hole (12). Also, a particle type distribution estimating program, which is a number deriving means, is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

公开(公告)号：US20200070169A1

公开(公告)日：2020-03-05

申请号：US16614707

申请日：2018-05-09

Applicant: OSAKA UNIVERSITY

Inventor： Wataru TONOMURA ,  Makusu TSUTSUI ,  Kazumichi YOKOTA ,  Akihide ARIMA ,  Masateru TANIGUCHI ,  Tomoji KAWAI

IPC: B01L3/00 ,  G01N1/40 ,  B01D29/44 ,  C12N1/20

Abstract: Provided is a channel device that is capable of increasing the concentration of fine particles in a liquid only by use of fluid-dynamic flows without relying on electrostatic interactions. A channel device (1) in accordance with an embodiment of the present invention includes: a main channel (11) configured to allow a liquid containing fine particles to flow therethrough; a chamber (15) that is provided at an end of the main channel (11) and that is configured to store target fine particles which have increased in concentration; and a side channel (12) that is connected to a side face of the main channel (11) and that is configured to allow unwanted liquid to drain therethrough, wherein at least one of a height and a width of the side channel (12) is smaller than a particle size of the fine particles.