公开(公告)号：US20190128888A1

公开(公告)日：2019-05-02

申请号：US16095340

申请日：2017-04-21

Applicant: OSAKA UNIVERSITY ,  TOKYO INSTITUTE OF TECHNOLOGY ,  National University Corporation Nagoya University ,  NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY

Inventor： Makusu TSUTSUI ,  Kazumichi YOKOTA ,  Masateru TANIGUCHI ,  Tomoji KAWAI ,  Mina OKOCHI ,  Masayoshi TANAKA ,  Yoshinobu BABA ,  Noritada KAJI ,  Takao YASUI ,  Yuji MIYAHARA ,  Yukichi HORIGUCHI

IPC: G01N33/569 ,  G01N33/566 ,  C12Q1/04 ,  C12Q1/68 ,  C07K7/08 ,  C07K7/06 ,  C12M1/26

Abstract: A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.

公开(公告)号：US20200033248A1

公开(公告)日：2020-01-30

申请号：US16340055

申请日：2017-10-04

Applicant: National University Corporation Nagoya University ,  OSAKA UNIVERSITY ,  KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION

Inventor： Hirotoshi YASAKI ,  Takao YASUI ,  Noritada KAJI ,  Yoshinobu BABA ,  Tomoji KAWAI ,  Satoyuki KAWANO ,  Kentaro DOI ,  Takeshi YANAGIDA ,  Mao FUKUYAMA

IPC: G01N15/10 ,  G01N27/327

Abstract: In a method for analyzing samples involving the use of a device for analyzing samples, the device for analyzing samples includes at least a movement part through which a sample moves, and a measurement unit that is formed in a middle of the movement part and that measures a value of an ion current when the sample passes through the movement part. The analysis method includes at least a measurement step for measuring the value of the ion current when the sample passes through the movement part, and a determination step for determining a change over time in a quantity of ions from the value of the ion current measured in the measurement step. The quantity of ions includes a quantity of ions that have leaked from the sample during movement of the sample through the movement part.

公开(公告)号：US20200251184A1

公开(公告)日：2020-08-06

申请号：US16470140

申请日：2017-12-12

Applicant: Osaka University

Inventor： Takashi WASHIO ,  Tomoji KAWAI ,  Masateru TANIGUCHI ,  Makusu TSUTSUI ,  Kazumichi YOKOTA ,  Akira ISHI ,  Takeshi YOSHIDA

IPC: G16B40/10 ,  G06K9/62 ,  G06N20/10 ,  G06F17/18 ,  G01N27/327 ,  G01N33/487

Abstract: The present invention provides a classification analysis method, a classification analysis device, and a storage medium for classification analysis, which enable, with high accuracy, the classification analysis of particulate or molecular analytes. As a means for solving the problem, a data group of particle-passage detection signals is based which are detected by a nanopore device 8 in accordance with passage of subject particles through a through-hole 12. A feature value is obtained in advance which indicates the feature of the waveform of the pulse signals corresponding to the passage of the predetermined analyte and the feature value obtained in advance is set as the learning data for the machine learning. The feature value obtained from the pulse signals of said analyzed data is set as a variable and the classification analysis on the predetermined analytes in the analyzed data can be performed by executing a classification analysis program due to the machine learning.

公开(公告)号：US20190257787A1

公开(公告)日：2019-08-22

申请号：US16065633

申请日：2016-12-19

Applicant: Osaka University

Inventor： Takashi WASHIO ,  Tomoji KAWAI ,  Masateru TANIGUCHI ,  Makusu TSUTSUI ,  Kazumichi YOKOTA ,  Akira ISHI ,  Takeshi YOSHIDA

IPC: G01N27/447 ,  G01N33/487

Abstract: The present invention provides a number analyzing method, a number analyzing device, and a storage medium for number analysis, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device (8) in accordance with passage of subject particles through a through-hole (12). Also, a particle type distribution estimating program, which is a number deriving means, is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

公开(公告)号：US20200070169A1

公开(公告)日：2020-03-05

申请号：US16614707

申请日：2018-05-09

Applicant: OSAKA UNIVERSITY

Inventor： Wataru TONOMURA ,  Makusu TSUTSUI ,  Kazumichi YOKOTA ,  Akihide ARIMA ,  Masateru TANIGUCHI ,  Tomoji KAWAI

IPC: B01L3/00 ,  G01N1/40 ,  B01D29/44 ,  C12N1/20

Abstract: Provided is a channel device that is capable of increasing the concentration of fine particles in a liquid only by use of fluid-dynamic flows without relying on electrostatic interactions. A channel device (1) in accordance with an embodiment of the present invention includes: a main channel (11) configured to allow a liquid containing fine particles to flow therethrough; a chamber (15) that is provided at an end of the main channel (11) and that is configured to store target fine particles which have increased in concentration; and a side channel (12) that is connected to a side face of the main channel (11) and that is configured to allow unwanted liquid to drain therethrough, wherein at least one of a height and a width of the side channel (12) is smaller than a particle size of the fine particles.

公开(公告)号：US20140374255A1

公开(公告)日：2014-12-25

申请号：US14484305

申请日：2014-09-12

Applicant: KABUSHIKI KAISHA TOSHIBA ,  OSAKA UNIVERSITY

Inventor： Sadato HONGO ,  Tomoji KAWAI ,  Makusu TSUTSUI ,  Masateru TANIGUCHI ,  Soh RYUZAKI

IPC: G01N27/447

CPC classification number: G01N27/44756 ,  G01N15/12 ,  G01N15/1218 ,  G01N33/48721 ,  G01N2015/1062 ,  G01N2015/1087 ,  G01N2015/1093

Abstract: According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t

Abstract translation: 根据一个实施例，提供了一种单颗粒分析装置，其包括测量容器，由绝缘膜限定的容器中的第一和第二室，用于连接室的膜中的孔口，以及室中的第一和第二电极。 电流通过孔流动在电极之间。 在目标从第一室迁移到第二室以测量目标的尺寸和形状时测量电特性。 （a）t