公开(公告)号：US5219730A

公开(公告)日：1993-06-15

申请号：US319578

申请日：1989-03-06

申请人： Pedro Potocnjak ,  Fidel Zavala ,  Ruth S. Nussenzweig ,  Victor Nussenzweig

发明人： Pedro Potocnjak ,  Fidel Zavala ,  Ruth S. Nussenzweig ,  Victor Nussenzweig

IPC分类号： G01N33/569 ,  G01N33/577 ,  G01N33/68

CPC分类号： G01N33/686 ,  G01N33/569 ,  G01N33/577

摘要： The present invention provides an immunoassay of general applicability. The assay detects an antigen by its inhibition of the reaction between the combining site of two antibodies. One of the antibodies binds antigen, and the second antibody binds to the combining site or idiotype of the first antibody. It is preferable that both the antibody that binds the antigen and the anti-idiotypic antibody are monoclonal antibodies. The assay provides a method for measuring concentrations of single epitopes without requiring purified antigen, and it is particularly useful for measuring the quantity of any nonpurified antigen present in low concentration in a mixture of antigens. The use of monoclonal antibodies as reagents is advantageous in providing an essentially unlimited supply of highly specific and standardized reagents, without the batch-to-batch variation encountered when using conventional polyclonal antibodies. The invention further provides a screening method for isolating the appropriate monoclonal antibodies of anti-idiotypic specificity.

摘要翻译： 本发明提供一般适用性的免疫测定法。 该测定通过其对两种抗体的组合位点之间的反应的抑制来检测抗原。 一种抗体结合抗原，第二抗体结合第一抗体的组合位点或独特型。 结合抗原和抗独特型抗体的抗体优选为单克隆抗体。 该测定提供了用于测量单一表位的浓度而不需要纯化抗原的方法，并且其特别可用于测量抗原混合物中低浓度存在的任何非纯化抗原的量。 使用单克隆抗体作为试剂有利于提供基本上无限量的高度特异性和标准化的试剂供应，而不使用常规多克隆抗体时遇到的批次间变化。 本发明还提供了分离抗独特型特异性的合适的单克隆抗体的筛选方法。

公开(公告)号：US5614194A

公开(公告)日：1997-03-25

申请号：US461945

申请日：1995-06-05

申请人： David R. Colman ,  Joan Ellis ,  G. Nigel Godson ,  Ruth S. Nussenzweig ,  Victor N. Nussenzweig ,  Pamela S. Svec ,  Fidel Zavala

发明人： David R. Colman ,  Joan Ellis ,  G. Nigel Godson ,  Ruth S. Nussenzweig ,  Victor N. Nussenzweig ,  Pamela S. Svec ,  Fidel Zavala

IPC分类号： A61K39/00 ,  C07K14/445 ,  C07K16/20 ,  C12N15/00 ,  C07K14/00 ,  C07K5/00

CPC分类号： C07K16/205 ,  C07K14/445 ,  A61K39/00

摘要： Described is a antigen and antigenic amino acid sequence consisting of repeating units of the immunodominant epitope region of the circumsporozoite surface protein of a parasite of the genus Plasmodium. Also described is a related fusion protein produced by a recombinant microorganism and a vaccine for immunizing mammals against malaria.

摘要翻译： 描述了由疟原虫寄生虫的环子孢子表面蛋白的免疫优势表位区的重复单元组成的抗原和抗原性氨基酸序列。 还描述了由重组微生物和用于免疫哺乳动物免疫疟疾的疫苗产生的相关融合蛋白。

公开(公告)号：US20220280632A1

公开(公告)日：2022-09-08

申请号：US17628640

申请日：2020-07-24

申请人： Bryce CHACKERIAN ,  David S. PEABODY ,  Nikolai PETROVSKY ,  Lucia JELINKOVA ,  Fidel ZAVALA ,  UNM RAINFOREST INNOVATIONS ,  THE JOHNS HOPKINS UNIVERSITY ,  VAXINE PTY LTD.

发明人： Bryce Chackerian ,  David S. Peabody ,  Nikolai Petrovsky ,  Lucia Jelinkova ,  Fidel Zavala

IPC分类号： A61K39/12 ,  C07K14/005 ,  C12N7/00 ,  A61P33/06

摘要： An immunogen useful for treating malaria generally includes an immunogenic carrier and an antigenic malaria circumsporozoite protein (CSP) peptide that includes the peptide NPDPNANPNVDPNAN (amino acids 5-19 of SEQ ID NO:1) linked to the immunogenic carrier. The immunogen may be administered to a subject having or at risk of having malaria.