公开(公告)号：US20220280632A1

公开(公告)日：2022-09-08

申请号：US17628640

申请日：2020-07-24

申请人： Bryce CHACKERIAN ,  David S. PEABODY ,  Nikolai PETROVSKY ,  Lucia JELINKOVA ,  Fidel ZAVALA ,  UNM RAINFOREST INNOVATIONS ,  THE JOHNS HOPKINS UNIVERSITY ,  VAXINE PTY LTD.

发明人： Bryce Chackerian ,  David S. Peabody ,  Nikolai Petrovsky ,  Lucia Jelinkova ,  Fidel Zavala

IPC分类号： A61K39/12 ,  C07K14/005 ,  C12N7/00 ,  A61P33/06

摘要： An immunogen useful for treating malaria generally includes an immunogenic carrier and an antigenic malaria circumsporozoite protein (CSP) peptide that includes the peptide NPDPNANPNVDPNAN (amino acids 5-19 of SEQ ID NO:1) linked to the immunogenic carrier. The immunogen may be administered to a subject having or at risk of having malaria.

公开(公告)号：US11530242B2

公开(公告)日：2022-12-20

申请号：US17259400

申请日：2019-07-16

申请人： UNM RAINFOREST INNOVATIONS ,  Bryce Chackerian ,  Oliver Rixe

发明人： Bryce Chackerian ,  Oliver Rixe

IPC分类号： A61K39/00 ,  A61K39/12 ,  C12N7/00 ,  C12N15/62 ,  A61K38/00 ,  C07K7/08 ,  A61K45/06

摘要： An immunogen includes an antigenic EGFRvIII peptide linked to a Qβ bacteriophage virus-like particle (VLP) carrier. The antigenic EGFRvIII peptide has at least 53%, at least 61%, at least 69%, at least 76%, at least 84%, or at least 92% sequence similarity amino acid similarity to LEEKKGNYVVTDH (SEQ ID NO:1).

公开(公告)号：US11633471B2

公开(公告)日：2023-04-25

申请号：US16977648

申请日：2019-03-06

申请人： UNM Rainforest Innovations ,  The United States of America, as represented by the Secretary, Department of Health and Human Services ,  Bryce Chackerian ,  Alan Remaley ,  Marcelo Amar ,  Alexandra Fowler

发明人： Bryce Chackerian ,  Alan Remaley ,  Marcelo Amar ,  Alexandra Fowler

IPC分类号： A61K39/385 ,  A61K47/69 ,  A61K39/00 ,  A61K45/06 ,  C12N7/00

摘要： An immunogen generally includes a virus-like particle and an antigen linked to the virus-like particle. The antigen includes an antigenic portion of a polypeptide, wherein the polypeptide inhibits lipoprotein lipase (LPL) activity by binding to LPL. In some embodiments, the polypeptide is at least a portion of angiopoietin-like 3 (ANGPTL3). In other embodiments, the polypeptide is at least a portion of angiopoietin-like 4 (ANGPTL4). In other embodiments, the polypeptide at least a portion of angiopoietin-like 8 (ANGPTL8). In some embodiments, the virus-like particle is a Qbeta immunogenic carrier. In some of these embodiments, the antigen is linked to the virus-like particle through a Gly-Gly-Gly-Cys linker at the C-terminal of the antigen.

公开(公告)号：US20210253641A1

公开(公告)日：2021-08-19

申请号：US17259400

申请日：2019-07-16

申请人： Bryce Chackerian ,  Oliver Rixe ,  UNM RAINFOREST INNOVATIONS

发明人： Bryce Chackerian ,  Oliver Rixe

IPC分类号： C07K7/08 ,  A61K45/06 ,  C12N7/00 ,  A61K39/00

摘要： An immunogen includes an antigenic EGFRvIII peptide linked to a Qβ bacteriophage virus-like particle (VLP) carrier. The antigenic EGFRvIII peptide has at least 53%, at least 61%, at least 69%, at least 76%, at least 84%, or at least 92% sequence similarity amino acid similarity to LEEKKGNYVVTDH (SEQ ID NO:1).

公开(公告)号：US20120308592A1

公开(公告)日：2012-12-06

申请号：US13577484

申请日：2011-02-08

申请人： Bryce Chackerian ,  David S. Peabody

发明人： Bryce Chackerian ,  David S. Peabody

IPC分类号： A61K39/12 ,  C12N15/63 ,  A61P31/20

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K47/6901 ,  A61K2039/5258 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2710/20023 ,  C12N2710/20034 ,  C12N2795/18123

摘要： The invention provides immunotherapeutic and prophylactic bacteriophage viral-like particle (VLPs) which are useful in the treatment and prevention of human papillomavirus (HPV) infections and related disorders, including cervical cancer and persistent infections associated with HPV. Related compositions (e.g. vaccines), nucleic acid constructs, and therapeutic methods are also provided. VLPs and related compositions of the invention induce high titer antibody responses against HPV L2 and protect against HPV challenge in vivo. VLPs, VLP-containing compositions, and therapeutic methods of the invention induce an immunogenic response against HPV infection, confer immunity against HPV infection, protect against HPV infection, and reduce the likelihood of infection by HPV infection.

摘要翻译： 本发明提供免疫治疗和预防性噬菌体病毒样颗粒（VLP），其可用于治疗和预防人乳头状瘤病毒（HPV）感染和相关疾病，包括宫颈癌和与HPV相关的持续感染。 还提供了相关组合物（例如疫苗），核酸构建体和治疗方法。 本发明的VLP和相关组合物诱导针对HPV L2的高滴度抗体应答，并在体内保护免受HPV挑战。 VLP，含VLP的组合物和本发明的治疗方法诱导针对HPV感染的免疫原性应答，赋予HPV感染免疫，防止HPV感染，并降低HPV感染感染的可能性。

公开(公告)号：US09803189B2

公开(公告)日：2017-10-31

申请号：US11895198

申请日：2007-08-23

申请人： David S. Peabody ,  Bryce Chackerian

发明人： David S. Peabody ,  Bryce Chackerian

IPC分类号： C40B40/02 ,  C12N15/10 ,  C07K14/005 ,  A61K39/00

CPC分类号： C12N15/1037 ,  A61K2039/5256 ,  A61K2039/5258 ,  C07K14/005 ,  C12N2740/16122 ,  C12N2795/18123 ,  C40B40/02

摘要： The invention is directed to virus-like particles (VLPs) of an RNA bacteriophage that (a) comprises a coat polypeptide of said phage modified by insertion of a heterologous peptide that is displayed on said VLP and (b) encapsidates said bacteriophage mRNA as well as populations of these VLPs, and their uses. The invention is further directed to VLPs that encapsidate heterologous substances, as well as populations of these VLPs and their uses.

公开(公告)号：US20140105924A1

公开(公告)日：2014-04-17

申请号：US13946562

申请日：2013-07-19

申请人： Bryce Chackerian ,  David S. Peabody ,  Ebenezer Tumban

发明人： Bryce Chackerian ,  David S. Peabody ,  Ebenezer Tumban

IPC分类号： A61K39/12

CPC分类号： A61K39/12 ,  A61K2039/5258 ,  A61K2039/58 ,  C12N2710/20034 ,  C12N2795/18023 ,  C12N2795/18071

摘要： The invention provides immunotherapeutic and prophylactic bacteriophage viral-like particle (VLPs) which are useful in the treatment and prevention of human papillomavirus (HPV) infections and related disorders, including cervical cancer and persistent infections associated with HPV. Related compositions (e.g. vaccines), nucleic acid constructs, and therapeutic methods are also provided. VLPs and related compositions of the invention induce high titer antibody responses against HPV L2 and protect against HPV challenge in vivo. VLPs, VLP-containing compositions, and therapeutic methods of the invention induce an immunogenic response against HPV infection, confer immunity against HPV infection, protect against HPV infection, and reduce the likelihood of infection by HPV infection.

摘要翻译： 本发明提供免疫治疗和预防性噬菌体病毒样颗粒（VLP），其可用于治疗和预防人乳头状瘤病毒（HPV）感染和相关疾病，包括宫颈癌和与HPV相关的持续感染。 还提供了相关组合物（例如疫苗），核酸构建体和治疗方法。 本发明的VLP和相关组合物诱导针对HPV L2的高滴度抗体应答，并在体内保护免受HPV挑战。 VLP，含VLP的组合物和本发明的治疗方法诱导针对HPV感染的免疫原性应答，赋予HPV感染免疫，防止HPV感染，并降低HPV感染感染的可能性。

公开(公告)号：US20110104203A1

公开(公告)日：2011-05-05

申请号：US12943709

申请日：2010-11-10

申请人： David S. PEABODY ,  Bryce Chackerian

发明人： David S. PEABODY ,  Bryce Chackerian

IPC分类号： A61K39/21 ,  C07H21/04 ,  C40B50/06 ,  C40B30/00 ,  C12P21/00 ,  A61K39/12 ,  A61P31/18 ,  A61P31/12

CPC分类号： C12N15/1037 ,  A61K2039/5256 ,  A61K2039/5258 ,  C07K14/005 ,  C12N2740/16122 ,  C12N2795/18123 ,  C40B40/02

摘要： The invention is directed to virus-like particles (VLPs) of an RNA bacteriophage that (a) comprises a coat polypeptide of said phage modified by insertion of a heterologous peptide that is displayed on said VLP and (b) encapsidates said bacteriophage mRNA as well as populations of these VLPs, and their uses. The invention is further directed to VLPs that encapsidate heterologous substances, as well as populations of these VLPs and their uses.

摘要翻译： 本发明涉及RNA噬菌体的病毒样颗粒（VLP），其（a）包含通过插入在所述VLP上显示的异源肽修饰的所述噬菌体的外壳多肽，和（b）还包封所述噬菌体mRNA 作为这些VLP的群体及其用途。 本发明还涉及包裹异源物质的VLP以及这些VLP的群体及其用途。

公开(公告)号：US09549976B1

公开(公告)日：2017-01-24

申请号：US14081629

申请日：2013-11-15

申请人： David S. Peabody ,  Bryce Chackerian ,  Carlee Ashley ,  Eric Carnes ,  Oscar Negrete

发明人： David S. Peabody ,  Bryce Chackerian ,  Carlee Ashley ,  Eric Carnes ,  Oscar Negrete

IPC分类号： A61K39/00 ,  A61K39/155 ,  A61K49/00 ,  A61K39/385 ,  C12Q1/70 ,  C12N7/00 ,  G01N33/569

CPC分类号： A61K39/155 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/5258 ,  C12N2760/18234 ,  G01N33/56983

摘要： The invention relates to virus-like particles of bacteriophage MS2 (MS2 VLPs) displaying peptide epitopes or peptide mimics of epitopes of Nipah Virus envelope glycoprotein that elicit an immune response against Nipah Virus upon vaccination of humans or animals. Affinity selection on Nipah Virus-neutralizing monoclonal antibodies using random sequence peptide libraries on MS2 VLPs selected peptides with sequence similarity to peptide sequences found within the envelope glycoprotein of Nipah itself, thus identifying the epitopes the antibodies recognize. The selected peptide sequences themselves are not necessarily identical in all respects to a sequence within Nipah Virus glycoprotein, and therefore may be referred to as epitope mimics VLPs displaying these epitope mimics can serve as vaccine. On the other hand, display of the corresponding wild-type sequence derived from Nipah Virus and corresponding to the epitope mapped by affinity selection, may also be used as a vaccine.

摘要翻译： 本发明涉及噬菌体MS2（MS2 VLP）的病毒样颗粒，其显示了尼泊尔病毒包膜糖蛋白的表位的肽表位或肽模拟物，其在接种人或动物时引起针对尼帕病毒的免疫应答。 在Nipah病毒中和单克隆抗体上的亲和力选择，使用MS2 VLP上的随机序列肽文库，选择与Nipah本身的包膜糖蛋白内发现的肽序列具有序列相似性的肽，从而鉴定抗体识别的表位。 所选择的肽序列本身在所有方面不一定与尼帕病毒糖蛋白内的序列相同，因此可以称为表位模拟物，显示这些表位模拟物的VLP可以用作疫苗。 另一方面，从Nipah Virus得到的相应野生型序列的显示与通过亲和力选择映射的表位对应，也可以用作疫苗。

公开(公告)号：US08992984B1

公开(公告)日：2015-03-31

申请号：US12909572

申请日：2010-10-21

申请人： C. Jeffrey Brinker ,  Carlee Erin Ashley ,  Xingmao Jiang ,  Juewen Liu ,  David S. Peabody ,  Walker Richard Wharton ,  Eric Carnes ,  Bryce Chackerian ,  Cheryl L. Willman

发明人： C. Jeffrey Brinker ,  Carlee Erin Ashley ,  Xingmao Jiang ,  Juewen Liu ,  David S. Peabody ,  Walker Richard Wharton ,  Eric Carnes ,  Bryce Chackerian ,  Cheryl L. Willman

IPC分类号： A61K9/14 ,  A61K9/51

CPC分类号： A61K9/127 ,  A61K9/1277 ,  A61K9/5115 ,  A61K9/5123 ,  A61K31/513 ,  A61K31/575 ,  A61K31/704 ,  A61K33/24 ,  A61K49/005

摘要： Various embodiments provide materials and methods for synthesizing protocells for use in targeted delivery of cargo components to cancer cells. In one embodiment, the lipid bilayer can be fused to the porous particle core to form a protocell. The lipid bilayer can be modified with targeting ligands or other ligands to achieve targeted delivery of cargo components that are loaded within the protocell to a target cell, e.g., a type of cancer. Shielding materials can be conjugated to the surface of the lipid bilayer to reduce undesired non-specific binding.

摘要翻译： 各种实施方案提供用于合成原细胞以用于向癌细胞靶向递送货物组分的材料和方法。 在一个实施方案中，脂质双层可以与多孔颗粒核心融合以形成原细胞。 脂质双层可以用靶向配体或其他配体进行修饰，以实现载体在原细胞内的货物组分的靶向递送到靶细胞，例如一种类型的癌症。 屏蔽材料可以结合到脂质双层的表面以减少不期望的非特异性结合。