公开(公告)号：US20180047552A1

公开(公告)日：2018-02-15

申请号：US15672862

申请日：2017-08-09

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Xin Yan ,  Christopher Pulliam ,  Ryan M. Bain ,  Tawnya Flick

IPC: H01J49/00 ,  H01J49/04 ,  G01N33/68 ,  H01J49/16

CPC classification number: H01J49/0031 ,  G01N33/6848 ,  H01J49/0409 ,  H01J49/165

Abstract: The invention generally relates to systems and methods for on-line reaction monitoring. In certain embodiments, the invention provides systems that include a reaction vessel having an outlet, a quantitation unit coupled to the outlet and configured to introduce internal standard and solvent into reaction solution flowed from the reaction vessel, one or more ion generating devices that receive flow from the quantitation unit, and a mass spectrometer. In certain embodiments, the invention provides systems for multiple reaction monitoring that include a plurality of reaction vessels, a plurality of ion generating devices, a plurality of channels, each channel coupling a reaction vessel to an ion generating device, an actuator coupled to the plurality of ion generating devices to thereby allow movement of the plurality of ion generating devices, and a mass spectrometer

公开(公告)号：US20220199383A1

公开(公告)日：2022-06-23

申请号：US17691294

申请日：2022-03-10

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Xin Yan ,  Christopher Pulliam ,  Ryan M. Bain ,  Tawnya Flick

IPC: H01J49/00 ,  H01J49/16 ,  H01J49/04 ,  G01N33/68

Abstract: The invention generally relates to systems and methods for on-line reaction monitoring. In certain embodiments, the invention provides systems that include a reaction vessel having an outlet, a quantitation unit coupled to the outlet and configured to introduce internal standard and solvent into reaction solution flowed from the reaction vessel, one or more ion generating devices that receive flow from the quantitation unit, and a mass spectrometer. In certain embodiments, the invention provides systems for multiple reaction monitoring that include a plurality of reaction vessels, a plurality of ion generating devices, a plurality of channels, each channel coupling a reaction vessel to an ion generating device, an actuator coupled to the plurality of ion generating devices to thereby allow movement of the plurality of ion generating devices, and a mass spectrometer

公开(公告)号：US20240087866A1

公开(公告)日：2024-03-14

申请号：US18511114

申请日：2023-11-16

Applicant: Purdue Research Foundation ,  AMGEN INC.

Inventor： Robert Graham Cooks ,  Tawnya Flick

IPC: H01J49/04 ,  G01N27/447 ,  H01J49/16

CPC classification number: H01J49/0431 ,  G01N27/44717 ,  G01N27/4473 ,  H01J49/165 ,  G01N30/7266

Abstract: The invention generally relates to electrophoretic mass spectrometry probes and systems and methods of uses thereof. In certain aspects, the invention provides a mass spectrometry probe having a hollow body with a distal tip, an electrically conductive hollow conduit, and an electrode. The electrically conductive hollow conduit may be operably coupled to a reservoir and a power source, and the electrically conductive hollow conduit may be configured to transport a liquid sample into the hollow body and polarize the liquid sample as it flows through the electrically conductive hollow conduit and into in the hollow body. The electrode and the electrically conductive hollow conduit are disposed within the hollow body (e.g., at different heights within the hollow body).

公开(公告)号：US20210223260A1

公开(公告)日：2021-07-22

申请号：US17158369

申请日：2021-01-26

Applicant: Purdue Research Foundation ,  AMGEN INC.

Inventor： Stephen T. Ayrton ,  Robert Graham Cooks ,  Tawnya Flick ,  Da Ren

IPC: G01N33/68 ,  H01J49/10 ,  H01J49/00

Abstract: The invention generally relates to methods for determining whether a peptide includes aspartate or isoaspartate. In certain aspects, methods of the invention involve binding an aspartate/isoaspartate residue in a peptide with a label to produce a labeled peptide. The labeled peptide is then ionized. The ionizing process causes the label to undergo rearrangement in a gas phase at a higher rate if the label is bound to the aspartate residue as compared to if the label is bound to the isoaspartate residue. The methods of the invention then involve performing a mass spectrometry analysis to detect the rearrangement of the label, thereby determining whether the peptide includes aspartate or isoaspartate.

公开(公告)号：US12142472B2

公开(公告)日：2024-11-12

申请号：US18511114

申请日：2023-11-16

Applicant: Purdue Research Foundation ,  AMGEN INC.

Inventor： Robert Graham Cooks ,  Tawnya Flick

IPC: H01J49/04 ,  G01N27/447 ,  H01J49/16 ,  G01N30/72

Abstract: The invention generally relates to electrophoretic mass spectrometry probes and systems and methods of uses thereof. In certain aspects, the invention provides a mass spectrometry probe having a hollow body with a distal tip, an electrically conductive hollow conduit, and an electrode. The electrically conductive hollow conduit may be operably coupled to a reservoir and a power source, and the electrically conductive hollow conduit may be configured to transport a liquid sample into the hollow body and polarize the liquid sample as it flows through the electrically conductive hollow conduit and into in the hollow body. The electrode and the electrically conductive hollow conduit are disposed within the hollow body (e.g., at different heights within the hollow body).

公开(公告)号：US11854781B2

公开(公告)日：2023-12-26

申请号：US17979121

申请日：2022-11-02

Applicant: Purdue Research Foundation ,  AMGEN INC.

Inventor： Robert Graham Cooks ,  Tawnya Flick

IPC: H01J49/04 ,  H01J49/16 ,  G01N27/44 ,  G01N27/447 ,  G01N30/72

CPC classification number: H01J49/0431 ,  G01N27/4473 ,  G01N27/44717 ,  H01J49/165 ,  G01N30/7266

Abstract: The invention generally relates to electrophoretic mass spectrometry probes and systems and methods of uses thereof. In certain aspects, the invention provides a mass spectrometry probe having a hollow body with a distal tip, an electrically conductive hollow conduit, and an electrode. The electrically conductive hollow conduit may be operably coupled to a reservoir and a power source, and the electrically conductive hollow conduit may be configured to transport a liquid sample into the hollow body and polarize the liquid sample as it flows through the electrically conductive hollow conduit and into in the hollow body. The electrode and the electrically conductive hollow conduit are disposed within the hollow body (e.g., at different heights within the hollow body).

公开(公告)号：US10908165B2

公开(公告)日：2021-02-02

申请号：US16002749

申请日：2018-06-07

Applicant: Purdue Research Foundation ,  AMGEN INC.

Inventor： Stephen T. Ayrton ,  Robert Graham Cooks ,  Tawnya Flick ,  Da Ren

IPC: G01N33/68 ,  H01J49/10 ,  H01J49/00

Abstract: The invention generally relates to methods for determining whether a peptide includes aspartate or isoaspartate. In certain aspects, methods of the invention involve binding an aspartate/isoaspartate residue in a peptide with a label to produce a labeled peptide. The labeled peptide is then ionized. The ionizing process causes the label to undergo rearrangement in a gas phase at a higher rate if the label is bound to the aspartate residue as compared to if the label is bound to the isoaspartate residue. The methods of the invention then involve performing a mass spectrometry analysis to detect the rearrangement of the label, thereby determining whether the peptide includes aspartate or isoaspartate.

公开(公告)号：US20230066637A1

公开(公告)日：2023-03-02

申请号：US17979121

申请日：2022-11-02

Applicant: Purdue Research Foundation ,  AMGEN INC.

Inventor： Robert Graham Cooks ,  Tawnya Flick

IPC: H01J49/04 ,  G01N27/447 ,  H01J49/16 ,  G01N30/72

Abstract: The invention generally relates to electrophoretic mass spectrometry probes and systems and methods of uses thereof. In certain aspects, the invention provides a mass spectrometry probe having a hollow body with a distal tip, an electrically conductive hollow conduit, and an electrode. The electrically conductive hollow conduit may be operably coupled to a reservoir and a power source, and the electrically conductive hollow conduit may be configured to transport a liquid sample into the hollow body and polarize the liquid sample as it flows through the electrically conductive hollow conduit and into in the hollow body. The electrode and the electrically conductive hollow conduit are disposed within the hollow body (e.g., at different heights within the hollow body).

公开(公告)号：US11309172B2

公开(公告)日：2022-04-19

申请号：US15672862

申请日：2017-08-09

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Xin Yan ,  Christopher Pulliam ,  Ryan M. Bain ,  Tawnya Flick

IPC: H01J49/00 ,  H01J49/16 ,  H01J49/04 ,  G01N33/68

Abstract: The invention generally relates to systems and methods for on-line reaction monitoring. In certain embodiments, the invention provides systems that include a reaction vessel having an outlet, a quantitation unit coupled to the outlet and configured to introduce internal standard and solvent into reaction solution flowed from the reaction vessel, one or more ion generating devices that receive flow from the quantitation unit, and a mass spectrometer. In certain embodiments, the invention provides systems for multiple reaction monitoring that include a plurality of reaction vessels, a plurality of ion generating devices, a plurality of channels, each channel coupling a reaction vessel to an ion generating device, an actuator coupled to the plurality of ion generating devices to thereby allow movement of the plurality of ion generating devices, and a mass spectrometer.

公开(公告)号：US20190019662A1

公开(公告)日：2019-01-17

申请号：US16020524

申请日：2018-06-27

Applicant: Purdue Research Foundation ,  AMGEN INC.

Inventor： Robert Graham Cooks ,  Tawnya Flick

IPC: H01J49/04 ,  G01N27/447

Abstract: The invention generally relates to electrophoretic mass spectrometry probes and systems and methods of uses thereof. In certain aspects, the invention provides a mass spectrometry probe having a hollow body with a distal tip, an electrically conductive hollow conduit, and an electrode. The electrically conductive hollow conduit may be operably coupled to a reservoir and a power source, and the electrically conductive hollow conduit may be configured to transport a liquid sample into the hollow body and polarize the liquid sample as it flows through the electrically conductive hollow conduit and into in the hollow body. The electrode and the electrically conductive hollow conduit are disposed within the hollow body (e.g., at different heights within the hollow body).