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1.发明授权
公开(公告)号：US5736330A
公开(公告)日：1998-04-07
申请号：US542401
申请日：1995-10-11
申请人： R. Jerrold Fulton
发明人： R. Jerrold Fulton
IPC分类号： C12Q1/68 , G01N15/10 , G01N15/14 , G01N33/50 , G01N33/543 , C12P1/68 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： G01N33/54313 , C12Q1/6827 , G01N15/1012 , G01N15/1456 , G01N33/5094 , G01N2015/1018 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2015/149
摘要： A method for the analysis of DNA sequences and PCR products comprises the steps of constructing an oligonucleotide-labeled bead set, and labeled complementary probe, and exposing the bead set and probe to a DNA fragment or PCR product under hybridizing conditions and analyzing the combined sample/bead set by flow cytometry. Flow cytometric measurements are used to classify beads within an exposed bead set to determine the presence of identical or nonidentical sequences within the test sample. The inventive technology enables the rapid analysis of DNA sequences and detection of point mutations, deletions and/or inversions while also reducing the cost and time for performing genetic assays.
摘要翻译： 分析DNA序列和PCR产物的方法包括以下步骤：在杂交条件下构建寡核苷酸标记的珠粒组和标记的互补探针，并将珠组和探针暴露于DNA片段或PCR产物，并分析组合的样品 /流式细胞仪设定的珠粒。 流式细胞术测量用于对暴露的珠粒组中的珠粒进行分类，以确定测试样品中相同或非相似序列的存在。 本发明的技术使得能够快速分析DNA序列并检测点突变，缺失和/或逆转，同时还降低了进行基因测定的成本和时间。
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公开(公告)号：US06939720B2
公开(公告)日：2005-09-06
申请号：US10329391
申请日：2002-12-27
IPC分类号： G01N15/10 , G01N15/14 , G01N33/50 , G01N33/543 , G01N33/553
CPC分类号： G01N33/54313 , G01N15/1012 , G01N15/1456 , G01N33/5094 , G01N2015/1018 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2015/149 , Y10S435/973
摘要： A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules or DNA sequences in real-time while also reducing the cost of performing diagnostic and genetic assays.
摘要翻译： 用于酶，DNA片段，抗体和其他生物分子的多重诊断和遗传分析的方法包括以下步骤：构建适当标记的珠粒，将珠粒暴露于临床样品，并通过流式细胞术分析组合的样品/珠粒。 流式细胞仪测量用于实时分类暴露珠粒内的珠子，并根据在实时分析中获得的累积数据，为用户生成文字说明。 本发明的技术使得能够实时地同时和自动地检测和解释多个生物分子或DNA序列，同时还降低了进行诊断和遗传测定的成本。
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公开(公告)号：US5981180A
公开(公告)日：1999-11-09
申请号：US540814
申请日：1995-10-11
IPC分类号： G01N15/10 , G01N15/14 , G01N33/50 , G01N33/543 , C12Q1/68
CPC分类号： G01N33/54313 , G01N15/1012 , G01N15/1456 , G01N33/5094 , B01J2219/00459 , B01J2219/00576 , G01N2015/1018 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2015/149 , Y10S435/973
摘要： A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules or DNA sequences in real-time while also reducing the cost of performing diagnostic and genetic assays.
摘要翻译： 用于酶，DNA片段，抗体和其他生物分子的多重诊断和遗传分析的方法包括以下步骤：构建适当标记的珠粒，将珠粒暴露于临床样品，并通过流式细胞术分析组合的样品/珠粒。 流式细胞仪测量用于实时分类暴露珠粒内的珠子，并根据在实时分析中获得的累积数据，为用户生成文字说明。 本发明的技术使得能够实时地同时和自动地检测和解释多个生物分子或DNA序列，同时还降低了进行诊断和遗传测定的成本。
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公开(公告)号：US06524793B1
公开(公告)日：2003-02-25
申请号：US09335719
申请日：1999-06-18
IPC分类号： G01N3353
CPC分类号： G01N33/54313 , G01N15/1012 , G01N15/1456 , G01N33/5094 , G01N2015/1018 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2015/149 , Y10S435/973
摘要： A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules or DNA sequences in real-time while also reducing the cost of performing diagnostic and genetic assays.
摘要翻译： 用于酶，DNA片段，抗体和其他生物分子的多重诊断和遗传分析的方法包括以下步骤：构建适当标记的珠粒，将珠粒暴露于临床样品，并通过流式细胞术分析组合的样品/珠粒。 流式细胞仪测量用于实时分类暴露珠粒内的珠子，并根据在实时分析中获得的累积数据，为用户生成文字说明。 本发明的技术使得能够实时地同时和自动地检测和解释多个生物分子或DNA序列，同时还降低了进行诊断和遗传测定的成本。
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5.发明授权
公开(公告)号：US6057107A
公开(公告)日：2000-05-02
申请号：US55329
申请日：1998-04-06
申请人： R. Jerrold Fulton
发明人： R. Jerrold Fulton
IPC分类号： C12Q1/68 , G01N15/10 , G01N15/14 , G01N33/50 , G01N33/543
CPC分类号： G01N33/54313 , C12Q1/6827 , G01N15/1012 , G01N15/1456 , G01N33/5094 , G01N2015/1018 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2015/149
摘要： A method for the analysis of DNA sequences and PCR products comprises the steps of constructing an oligonucleotide-labeled beadset, and labeled complementary probe, and exposing the beadset and probe to a DNA fragment or PCR product under hybridizing conditions and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify beads within an exposed beadset to determine the presence of identical or nonidentical sequences within the test sample. The inventive technology enables the rapid analysis of DNA sequences and detection of point mutations, deletions and/or inversions while also reducing the cost and time for performing genetic assays.
摘要翻译： 分析DNA序列和PCR产物的方法包括以下步骤：构建寡核苷酸标记的珠粒和标记的互补探针，并在杂交条件下将珠粒和探针暴露于DNA片段或PCR产物，并分析组合的样品/珠粒 通过流式细胞术。 流式细胞术测量用于对暴露的珠粒中的珠粒进行分类，以确定测试样品中相同或非相似序列的存在。 本发明的技术使得能够快速分析DNA序列并检测点突变，缺失和/或逆转，同时还降低了进行基因测定的成本和时间。
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