公开(公告)号：US20240110932A1

公开(公告)日：2024-04-04

申请号：US18541624

申请日：2023-12-15

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Johannes Pflieger ,  Nicole Pirkl ,  Martin Rempt ,  Christoph Zuth

IPC: G01N33/74 ,  G01N30/72 ,  G01N33/68 ,  H01J49/00

CPC classification number: G01N33/743 ,  G01N30/7233 ,  G01N33/6848 ,  H01J49/0009 ,  G01N2030/027 ,  G01N2333/575 ,  G01N2496/00

Abstract: The present invention relates to a method of determining an analyte in a sample by mass spectrometry (MS), the method comprising (a) admixing a pre-determined amount of internal calibrator to said sample, wherein said internal calibrator comprises at least two non-identical isotopologues of the analyte at predetermined amounts; (b) determining MS signals of ions generated from said analyte (analyte signal) and from said at least two isotopologues (isotopologue signals); (c) providing a calibration based on the analyte signal and the isotopologue signals determined in step (b); and (d) determining said analyte based on the calibration provided in step (c). Further, the present invention relates to devices, systems, and uses related thereto.