公开(公告)号：US20180030432A1

公开(公告)日：2018-02-01

申请号：US15547216

申请日：2015-01-30

Applicant: Shimadzu Corporation ,  KAZUSA DNA RESEARCH INSTITUTE

Inventor： Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Shin NAKAMURA ,  Osamu OHARA

IPC: C12N15/10 ,  B03C1/28 ,  B01J19/08

CPC classification number: C12N15/1013 ,  B01J19/087 ,  B01J2219/085 ,  B01J2219/089 ,  B03C1/01 ,  B03C1/28 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  C12Q1/68

Abstract: A device for handling of magnetic particles, in which liquids and a gel-like medium are loaded. The device is provided with: a first liquid containing part in which a first liquid is contained; a second liquid contained, part in which a second liquid is contained, a third liquid containing part in which a third liquid is contained, and a first gel-like medium containing part in which the first gel-like medium is contained. The first liquid containing part, the second liquid containing part and the third liquid containing part are connected to the first gel-like medium containing part. The first liquid, the second liquid and the third liquid are separated from each other by the first gel-like medium.

公开(公告)号：US20220355310A1

公开(公告)日：2022-11-10

申请号：US17840568

申请日：2022-06-14

Applicant: SHIMADZU CORPORATION

Inventor： Masamitsu SHIKATA ,  Ayaka MINAMIMOTO ,  Akira MURAMATSU ,  Masaki KANAI ,  Seiya FUJIWARA ,  Tetsuo OHASHI ,  Hiroyuki JIKUYA

IPC: B03C1/12 ,  C12Q1/6865 ,  B01D15/38

Abstract: A manufacturing method of an operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, a manufacturing method of an operation pipe, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.

公开(公告)号：US20190024142A1

公开(公告)日：2019-01-24

申请号：US16071006

申请日：2017-01-18

Applicant: SHIMADZU CORPORATION ,  KAZUSA DNA RESEARCH INSTITUTE

Inventor： Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Shin NAKAMURA ,  Osamu OHARA

IPC: C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6834 ,  C12M1/26 ,  C12N15/10 ,  C12Q1/6816

Abstract: A pretreatment kit includes a container (10) for particle manipulation, nucleic acid capture particles (70) capable of selectively binding to nucleic acid, aqueous-phase separation medium (21, 22, 23), a plurality of kinds of aqueous liquids (35, 31, 32, 38), and a nucleic acid for individual identification. The nucleic acid for individual identification is contained in at least one of the plurality of kinds of aqueous liquids or is bound onto the surfaces of the nucleic acid capture particles. The base sequence of the nucleic acid for individual identification contains an identification sequence including a base sequence noncomplementary to the nucleic acids contained in the biological sample. The pretreatment kit is used for separating nucleic acids from biological samples that contains nucleic acids and contaminants.

公开(公告)号：US20220002709A1

公开(公告)日：2022-01-06

申请号：US17292941

申请日：2018-11-22

Applicant: SHIMADZU CORPORATION

Inventor： Seiya FUJIWARA ,  Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Masamitsu SHIKATA ,  Ayaka MINAMIMOTO

IPC: C12N15/10 ,  B01J19/08 ,  B03C1/005 ,  B03C1/28

Abstract: A purpose of the present invention is to suppress reduction in recovery rate of a target component due to a gel adhering to an inner wall surface of a treatment liquid layer. After passing magnetic particles through a gel layer, when moving the magnetic particles in the treatment liquid layer adjacent to the gel layer, the magnetic force source is automatically operated in a longitudinal direction of a tubular container so that the magnetic particles do not enter a range of a certain distance from the gel layer through which the magnetic particles have passed.

公开(公告)号：US20210129158A1

公开(公告)日：2021-05-06

申请号：US16603235

申请日：2017-04-06

Applicant: Shimadzu Corporation

Inventor： Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Seiya FUJIWARA

IPC: B03C1/32 ,  C12Q1/6806 ,  G01N35/08

Abstract: In a magnetic particle operation device 1, a plurality of liquid layers 11 and a plurality of gel-like medium layers are alternately arranged. Magnetic particles 13 are introduced into the uppermost liquid layer 11 of the magnetic particle operation device 1, and a holding magnet 60 is in contact with the outer surface of the bulging portion 21 of the container 20. Therefore, during the storage of the device 1, the magnetic particles 13 in the bulging portion 21 are aggregated and held at a position facing the holding magnet 60 in the sample introduction space by the magnetic force of the holding magnet 60. As a result, during the storage of the device 1, the magnetic particles 13 can be prevented from being brought into contact with the gel-like medium layer 12. Further, when the device 1 is used, the magnetic particles 13 can be dispersed in the liquid layer 11 by moving the holding magnet 60 away from the container 20.

公开(公告)号：US20210139954A1

公开(公告)日：2021-05-13

申请号：US17155158

申请日：2021-01-22

Applicant: SHIMADZU CORPORATION ,  KAZUSA DNA RESEARCH INSTITUTE

Inventor： Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Shin NAKAMURA ,  Osamu OHARA

IPC: C12Q1/6806 ,  C12N15/09 ,  C12N15/10 ,  C12M1/26 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q1/6876

Abstract: A pretreatment kit includes a container (10) for particle manipulation, nucleic acid capture particles (70) capable of selectively binding to nucleic acid, aqueous-phase separation medium (21, 22, 23), a plurality of kinds of aqueous liquids (35, 31, 32, 38), and a nucleic acid for individual identification. The nucleic acid for individual identification is contained in at least one of the plurality of kinds of aqueous liquids or is bound onto the surfaces of the nucleic acid capture particles. The base sequence of the nucleic acid for individual identification contains an identification sequence including a base sequence noncomplementary to the nucleic acids contained in the biological sample. The pretreatment kit is used for separating nucleic acids from biological samples that contains nucleic acids and contaminants.

公开(公告)号：US20200282407A1

公开(公告)日：2020-09-10

申请号：US16782064

申请日：2020-02-05

Applicant: SHIMADZU CORPORATION

Inventor： Masamitsu SHIKATA ,  Ayaka MINAMIMOTO ,  Akira MURAMATSU ,  Masaki KANAI ,  Seiya FUJIWARA ,  Tetsuo OHASHI ,  Hiroyuki JIKUYA

IPC: B03C1/12 ,  C12Q1/6865 ,  B01D15/38

Abstract: An operation pipe and a device equipped with the operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, an operation pipe and a device, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.

公开(公告)号：US20180224442A1

公开(公告)日：2018-08-09

申请号：US15891509

申请日：2018-02-08

Applicant: SHIMADZU CORPORATION

Inventor： Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Seiya FUJIWARA

IPC: G01N33/543 ,  G01N1/34

Abstract: To provide a device for operating magnetic particles capable of treating large quantity, and retaining a gel-like medium layer stably, a vessel is formed so that an area perpendicular to a longitudinal direction in a large-diameter part packed with a liquid layer is larger than an area perpendicular to the longitudinal direction in the small-diameter part packed with a gel-like medium layer. Therefore, in the vessel, it is possible to reduce the diameter of the small-diameter part while keeping the capacity of the large-diameter part large. As a result, in the vessel, it is possible to treat large quantity in the liquid layer, and it is possible to retain the gel-like medium layer stably.