公开(公告)号：US20180030432A1

公开(公告)日：2018-02-01

申请号：US15547216

申请日：2015-01-30

Applicant: Shimadzu Corporation ,  KAZUSA DNA RESEARCH INSTITUTE

Inventor： Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Shin NAKAMURA ,  Osamu OHARA

IPC: C12N15/10 ,  B03C1/28 ,  B01J19/08

CPC classification number: C12N15/1013 ,  B01J19/087 ,  B01J2219/085 ,  B01J2219/089 ,  B03C1/01 ,  B03C1/28 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  C12Q1/68

Abstract: A device for handling of magnetic particles, in which liquids and a gel-like medium are loaded. The device is provided with: a first liquid containing part in which a first liquid is contained; a second liquid contained, part in which a second liquid is contained, a third liquid containing part in which a third liquid is contained, and a first gel-like medium containing part in which the first gel-like medium is contained. The first liquid containing part, the second liquid containing part and the third liquid containing part are connected to the first gel-like medium containing part. The first liquid, the second liquid and the third liquid are separated from each other by the first gel-like medium.

公开(公告)号：US20160201114A1

公开(公告)日：2016-07-14

申请号：US14901791

申请日：2013-07-03

Applicant: SHIMADZU CORPORATION ,  KAZUSA DNA RESEARCH INSTITUTE

Inventor： Tetsuo OHASHI ,  Osamu Ohara ,  Ken Nonaka

IPC: C12Q1/68 ,  B01D15/18 ,  C12N15/10

CPC classification number: B01D12/00 ,  B01D15/1892 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/288 ,  B03C2201/26 ,  C07K1/145 ,  C12N15/1013 ,  C12Q1/6806

Abstract: Disclosed is a particle manipulation method including steps of moving particles, which exist in a water-based liquid, into a gelled medium that is insoluble or hardly soluble in the water-based liquid in the water-based liquid, and moving the particles, which exist in the gelled medium, to the outside of the gelled medium. Preferably, the gelled medium is a gel that contains chemically crosslinking polymer. Preferably, the gelled medium has a consistency of 340 to 475. In one embodiment, the movement of the particles existing in the water-based liquid to the gelled medium and the movement of the particles existing inside the gelled medium to the water-based liquid are carried out inside a device, the device being loaded with a plurality of water-based liquids and a gelled medium interposed among the water-based liquids.

Abstract translation: 公开了一种粒子处理方法，包括以下步骤：将存在于水基液体中的颗粒移动到水基液体中的水性液体中不溶或难溶的胶凝介质中，并且使颗粒移动 存在于凝胶介质中，到凝胶介质的外部。 优选地，凝胶介质是含有化学交联聚合物的凝胶。 优选地，凝胶介质的稠度为340至475.在一个实施方案中，存在于水基液体中的颗粒到凝胶介质的移动以及存在于凝胶介质内的颗粒向水性液体的移动 在装置内进行，该装置装载有多个水基液体和介于水基液体之间的凝胶介质。

公开(公告)号：US20220355310A1

公开(公告)日：2022-11-10

申请号：US17840568

申请日：2022-06-14

Applicant: SHIMADZU CORPORATION

Inventor： Masamitsu SHIKATA ,  Ayaka MINAMIMOTO ,  Akira MURAMATSU ,  Masaki KANAI ,  Seiya FUJIWARA ,  Tetsuo OHASHI ,  Hiroyuki JIKUYA

IPC: B03C1/12 ,  C12Q1/6865 ,  B01D15/38

Abstract: A manufacturing method of an operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, a manufacturing method of an operation pipe, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.

公开(公告)号：US20190024142A1

公开(公告)日：2019-01-24

申请号：US16071006

申请日：2017-01-18

Applicant: SHIMADZU CORPORATION ,  KAZUSA DNA RESEARCH INSTITUTE

Inventor： Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Shin NAKAMURA ,  Osamu OHARA

IPC: C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6834 ,  C12M1/26 ,  C12N15/10 ,  C12Q1/6816

Abstract: A pretreatment kit includes a container (10) for particle manipulation, nucleic acid capture particles (70) capable of selectively binding to nucleic acid, aqueous-phase separation medium (21, 22, 23), a plurality of kinds of aqueous liquids (35, 31, 32, 38), and a nucleic acid for individual identification. The nucleic acid for individual identification is contained in at least one of the plurality of kinds of aqueous liquids or is bound onto the surfaces of the nucleic acid capture particles. The base sequence of the nucleic acid for individual identification contains an identification sequence including a base sequence noncomplementary to the nucleic acids contained in the biological sample. The pretreatment kit is used for separating nucleic acids from biological samples that contains nucleic acids and contaminants.

公开(公告)号：US20180112207A1

公开(公告)日：2018-04-26

申请号：US15543425

申请日：2015-01-15

Applicant: SHIMADZU CORPORATION

Inventor： Masaki KANAI ,  Tetsuo OHASHI ,  Takahiro NISHIMOTO

IPC: C12N15/10 ,  C07K1/14 ,  B01L3/00 ,  C07K16/00 ,  B03C1/28

CPC classification number: C12N15/1013 ,  B01L3/502 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0832 ,  B01L2300/0838 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26 ,  C07K1/14 ,  C07K16/00

Abstract: A device for manipulating magnetic particles includes a gelled medium layer and liquid layers alternately stacked in a tubular container along a longitudinal direction of the container. A magnetic particle movement portion for moving magnetic particles exists along an inner wall surface of the container, and the magnetic particle movement portion extends along the longitudinal direction of the container. At a portion where the gelled medium layer is loaded, the cross-sectional shape of the container inner wall in a plane perpendicular to the longitudinal direction of the container is non-circular, and the shape of the magnetic particle movement portion in the cross section is a curved shape or an angular shape.

公开(公告)号：US20210139954A1

公开(公告)日：2021-05-13

申请号：US17155158

申请日：2021-01-22

Applicant: SHIMADZU CORPORATION ,  KAZUSA DNA RESEARCH INSTITUTE

Inventor： Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Shin NAKAMURA ,  Osamu OHARA

IPC: C12Q1/6806 ,  C12N15/09 ,  C12N15/10 ,  C12M1/26 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q1/6876

Abstract: A pretreatment kit includes a container (10) for particle manipulation, nucleic acid capture particles (70) capable of selectively binding to nucleic acid, aqueous-phase separation medium (21, 22, 23), a plurality of kinds of aqueous liquids (35, 31, 32, 38), and a nucleic acid for individual identification. The nucleic acid for individual identification is contained in at least one of the plurality of kinds of aqueous liquids or is bound onto the surfaces of the nucleic acid capture particles. The base sequence of the nucleic acid for individual identification contains an identification sequence including a base sequence noncomplementary to the nucleic acids contained in the biological sample. The pretreatment kit is used for separating nucleic acids from biological samples that contains nucleic acids and contaminants.

公开(公告)号：US20200282407A1

公开(公告)日：2020-09-10

申请号：US16782064

申请日：2020-02-05

Applicant: SHIMADZU CORPORATION

Inventor： Masamitsu SHIKATA ,  Ayaka MINAMIMOTO ,  Akira MURAMATSU ,  Masaki KANAI ,  Seiya FUJIWARA ,  Tetsuo OHASHI ,  Hiroyuki JIKUYA

IPC: B03C1/12 ,  C12Q1/6865 ,  B01D15/38

Abstract: An operation pipe and a device equipped with the operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, an operation pipe and a device, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.

公开(公告)号：US20180224442A1

公开(公告)日：2018-08-09

申请号：US15891509

申请日：2018-02-08

Applicant: SHIMADZU CORPORATION

Inventor： Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Seiya FUJIWARA

IPC: G01N33/543 ,  G01N1/34

Abstract: To provide a device for operating magnetic particles capable of treating large quantity, and retaining a gel-like medium layer stably, a vessel is formed so that an area perpendicular to a longitudinal direction in a large-diameter part packed with a liquid layer is larger than an area perpendicular to the longitudinal direction in the small-diameter part packed with a gel-like medium layer. Therefore, in the vessel, it is possible to reduce the diameter of the small-diameter part while keeping the capacity of the large-diameter part large. As a result, in the vessel, it is possible to treat large quantity in the liquid layer, and it is possible to retain the gel-like medium layer stably.

公开(公告)号：US20170073667A1

公开(公告)日：2017-03-16

申请号：US15125501

申请日：2014-03-14

Applicant: SHIMADZU CORPORATION

Inventor： Tetsuo OHASHI ,  Yukio OIKAWA

IPC: C12N15/10 ,  B01L3/00 ,  B03C1/30 ,  B03C1/01 ,  B03C1/033

Abstract: A magnetic particle manipulation method for magnetic particle comprises the steps of; subjecting magnetic particles and a magnetic solid having a larger particle diameter than said magnetic particles to existing together in a liquid layer, and moving said magnetic solid and said magnetic particles in said liquid layer by a magnetic field manipulation. According to one aspect of the present invention; a manipulation method for magnetic particles comprising the steps of; moving magnetic particles in a first liquid layer into a gelled medium layer by a magnetic field manipulation in a device, wherein gelled medium layers and liquid layers are in-place alternately in a container, moving said magnetic particles present in the gelled medium into a second liquid layer by the magnetic field manipulation; and moving said magnetic particles along with said magnetic solid in the second liquid layer.

Abstract translation: 磁性粒子的磁性粒子处理方法包括以下步骤： 将具有比所述磁性颗粒大的粒径的磁性颗粒和磁性固体一起存在于液体层中，并通过磁场操作使所述液体层中的所述磁性固体和所述磁性颗粒移动。 根据本发明的一个方面; 一种磁性粒子的操作方法，包括以下步骤： 通过在装置中的磁场操作将第一液体层中的磁性颗粒移动到凝胶介质层中，其中凝胶介质层和液体层在容器中交替存在，将存在于凝胶介质中的磁性颗粒移动到第二液体 液面由磁场操纵; 以及将所述磁性颗粒与所述磁性固体一起移动到所述第二液体层中。

公开(公告)号：US20220002709A1

公开(公告)日：2022-01-06

申请号：US17292941

申请日：2018-11-22

Applicant: SHIMADZU CORPORATION

Inventor： Seiya FUJIWARA ,  Masaki KANAI ,  Hiroyuki JIKUYA ,  Tetsuo OHASHI ,  Masamitsu SHIKATA ,  Ayaka MINAMIMOTO

IPC: C12N15/10 ,  B01J19/08 ,  B03C1/005 ,  B03C1/28

Abstract: A purpose of the present invention is to suppress reduction in recovery rate of a target component due to a gel adhering to an inner wall surface of a treatment liquid layer. After passing magnetic particles through a gel layer, when moving the magnetic particles in the treatment liquid layer adjacent to the gel layer, the magnetic force source is automatically operated in a longitudinal direction of a tubular container so that the magnetic particles do not enter a range of a certain distance from the gel layer through which the magnetic particles have passed.