公开(公告)号：US20050124564A1

公开(公告)日：2005-06-09

申请号：US10818720

申请日：2004-04-05

Applicant: Katie Binley ,  Susan Kingsman ,  Stuart Naylor

Inventor： Katie Binley ,  Susan Kingsman ,  Stuart Naylor

IPC: A61K48/00 ,  C07K14/505 ,  C12N15/864 ,  C12N15/861 ,  C12N15/867

CPC classification number: C12N15/86 ,  A61K31/70 ,  A61K38/1816 ,  A61K48/00 ,  C07K14/505 ,  C12N2750/14143 ,  C12N2799/025 ,  C12N2830/002 ,  C12N2830/008

Abstract: Disclosed is a viral vector containing a nucleic acid sequence encoding erythropoietin (Epo), in operable linkage with an HRE expression control sequence, as well as uses of the vector; for instance, in preparing a medicament. Also provided are methods for treating anemia, can involve administering the vector to a patient, wherein expression of Epo is physiologically regulated such that hematocrit levels of the patient are corrected and maintained.

Abstract translation: 公开了含有编码红细胞生成素（Epo）的核酸序列的病毒载体，其与HRE表达控制序列可操作地连接，以及载体的用途; 例如，在制备药物中。 还提供了治疗贫血的方法，可以涉及向患者施用载体，其中Epo的表达在生理学上被调节，使得患者的血细胞比容水平被校正和维持。

公开(公告)号：US20080099348A1

公开(公告)日：2008-05-01

申请号：US11883296

申请日：2006-01-30

Applicant: Stuart Naylor

Inventor： Stuart Naylor

IPC: B65D81/20

CPC classification number: G01N1/2226 ,  B65D81/2076 ,  G01N2001/2241

Abstract: Apparatus is described for storing sensitive materials in containers (32) which are sealed and in which the internal atmosphere may be purged by flushing with an inert gas fed in from a purger unit (30) via a suitable feed lead (35). The gas exhausted from inside the container (32) is fed back into the purge unit (30) and then checked against a desired parameter, for example oxygen concentration, until the value of that parameter reaches a desired level. Purging may then be stopped and the container left sealed, preferably at a slight over pressure relative to the ambient atmosphere. The use of a separate purge unit enables many containers to be sequentially and effectively processed. The containers are particularly useful for storing sensitive chemical and biological samples.

Abstract translation: 描述了用于将敏感材料存储在容器（32）中的装置，所述容器（32）被密封，并且可以通过经由适当的进料引线（35）从净化器单元（30）进料的惰性气体冲洗来清洗内部气氛。 从容器（32）内部排出的气体被反馈到清洗单元（30）中，然后检查所期望的参数，例如氧浓度，直到该参数的值达到期望水平。 然后可以停止清洗，并且容器保持密封，优选地相对于环境气氛稍微过压。 使用单独的吹扫单元可以顺序地和有效地处理许多容器。 这些容器特别适用于储存敏感的化学和生物样品。

公开(公告)号：US06800281B2

公开(公告)日：2004-10-05

申请号：US10008610

申请日：2001-11-08

Applicant: Patrick Aebischer ,  Susan Mary Kingsman ,  Stuart Naylor ,  Nicholas Mazarakis

Inventor： Patrick Aebischer ,  Susan Mary Kingsman ,  Stuart Naylor ,  Nicholas Mazarakis

IPC: A61K4800

CPC classification number: C12N15/86 ,  A61K48/00 ,  C07K14/4756 ,  C12N2740/15043

Abstract: Disclosed and claimed are methods for treating or preventing neurodegenerative diseases, conditions or maladies or symptoms or physiology associated therewith, such as treating or preventing Parkinson's disease or symptoms or physiology associated therewith such as motor deficits or nigrostriatal degeneration; or, for inducing nigrostriatal regeneration. Advantageously, the methods involve administering a lentiviral vector that expresses GDNF, such as human GDNF, or a variant, homolog, analog or derivative thereof.

Abstract translation: 公开并要求保护的是用于治疗或预防神经变性疾病，病症或疾病或症状或与之相关的生理学的方法，例如治疗或预防帕金森病或与之相关的症状或生理学，例如运动缺陷或黑质纹状体变性; 或用于诱导黑质纹状体再生。 有利地，所述方法包括施用表达GDNF的慢病毒载体，例如人GDNF，或其变体，同系物，类似物或衍生物。

公开(公告)号：US20100124534A1

公开(公告)日：2010-05-20

申请号：US12503512

申请日：2009-07-15

Applicant: Richard Harrop ,  William Shingler ,  Stuart Naylor ,  Michael McDonald

Inventor： Richard Harrop ,  William Shingler ,  Stuart Naylor ,  Michael McDonald

IPC: A61K49/00 ,  A61P37/04

CPC classification number: G01N33/6854 ,  A61K39/0011 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  C12N2799/023 ,  G01N33/574 ,  G01N33/57419 ,  G01N33/57438 ,  G01N2800/52

Abstract: The present invention provides a method of monitoring the efficacy of an immunotherapy in a mammalian subject, wherein the subject has been administered an immunotherapy, wherein the immunotherapy comprises a viral vector containing a polynucleotide encoding an antigen, wherein the viral vector is capable of transducing cells in the mammalian subject to cause the cells to express the antigen; the method comprising: (b) measuring, from a biological sample isolated from the subject, an immune response of the subject to the antigen and comparing the immune response of the subject to the antigen to a reference measurement of immune response to the antigen; (c) measuring, from a biological sample isolated from the subject, an immune response of the subject to the viral vector and comparing the immune response of the subject to the viral vector to a reference measurement of immune response to the viral vector; and (d) determining efficacy based on the comparisons of (b) and (c), wherein an elevated immune response to the antigen and a reduced immune response to the viral vector are indicative of an effective immunotherapy.

Abstract translation: 本发明提供了监测哺乳动物受试者的免疫治疗功效的方法，其中所述受试者已经进行免疫治疗，其中所述免疫疗法包含含有编码抗原的多核苷酸的病毒载体，其中所述病毒载体能够转导细胞 在哺乳动物受试者中使细胞表达抗原; 该方法包括：（b）从分离自受试者的生物样品中测量受试者对抗原的免疫应答并将受试者与抗原的免疫应答与抗原的免疫应答的参考测量进行比较; （c）从分离自受试者的生物样品中测量受试者对病毒载体的免疫应答，并将受试者与病毒载体的免疫应答与对病毒载体的免疫应答的参考测量进行比较; 和（d）基于（b）和（c）的比较确定功效，其中对抗原的免疫应答升高和对病毒载体的免疫应答降低是指示有效的免疫治疗。

公开(公告)号：US09212786B2

公开(公告)日：2015-12-15

申请号：US13261113

申请日：2010-06-30

Applicant: Stuart Naylor

Inventor： Stuart Naylor

IPC: H01L21/673 ,  F17D1/04

CPC classification number: F17D1/04 ,  H01L21/67393

Abstract: Apparatus for purging containers for the storage of sensitive materials is described. It includes means to connect a source of compressed purge gas to a purge gas inlet (2) on a container (1), including a pneumatically operated valve (6) adapted to allow purge gas to pass from the source to the inlet and a timing device (9) associated with the valve (6) and adapted to turn off the gas flow after a given time, and including means to vary the time between commencement and cessation of gas flow. The timing device is preferably one which operates on a pneumatic or clockwork basis, enabling the apparatus to operate without any electrical power supply, driven by the purge gas pressure alone. This enables the construction of a simple reliable unit, at a cost even enabling the unit to be incorporated into the container itself if desired. The use of a pneumatically operated valve enables portable apparatus to be designed with low power use if it includes e.g. electronic sensors to measure a property of the purge gas being exhausted from the container.

Abstract translation: 描述了用于清洁用于存储敏感材料的容器的装置。 其包括将压缩的净化气体源连接到容器（1）上的吹扫气体入口（2）的装置，所述吹扫气体入口（2）包括气动操作的阀（6），其适于使吹扫气体从所述源传送到所述入口， 与阀（6）相关联并适于在给定时间之后关闭气流的装置（9），并且包括改变气体流量的开始和停止之间的时间的装置。 定时装置优选是以气动或发条为基础运行的定时装置，使得装置能够在没有任何电力供应的情况下操作，仅由吹扫气体压力驱动。 这使得能够构造简单可靠的单元，但是如果需要，甚至能够将该单元结合到容器本身中。 使用气动阀使得便携式设备可以被设计成具有低功率使用，如果它包括例如 用于测量从容器中排出的净化气体的性质的电子传感器。

公开(公告)号：US20120096740A1

公开(公告)日：2012-04-26

申请号：US13261113

申请日：2010-06-30

Applicant: Stuart Naylor

Inventor： Stuart Naylor

IPC: F26B21/06

CPC classification number: F17D1/04 ,  H01L21/67393

Abstract: Apparatus for purging containers for the storage of sensitive materials is described. It includes means to connect a source of compressed purge gas to a purge gas inlet (2) on a container (1), including a pneumatically operated valve (6) adapted to allow purge gas to pass from the source to the inlet and a timing device (9) associated with the valve (6) and adapted to turn off the gas flow after a given time, and including means to vary the time between commencement and cessation of gas flow. The timing device is preferably one which operates on a pneumatic or clockwork basis, enabling the apparatus to operate without any electrical power supply, driven by the purge gas pressure alone. This enables the construction of a simple reliable unit, at a cost even enabling the unit to be incorporated into the container itself if desired. The use of a pneumatically operated valve enables portable apparatus to be designed with low power use if it includes e.g. electronic sensors to measure a property of the purge gas being exhausted from the container.

Abstract translation: 描述了用于清洁用于存储敏感材料的容器的装置。 其包括将压缩的净化气体源连接到容器（1）上的吹扫气体入口（2）的装置，所述吹扫气体入口（2）包括气动操作的阀（6），其适于使吹扫气体从所述源传送到所述入口， 与阀（6）相关联并适于在给定时间之后关闭气流的装置（9），并且包括改变气体流量的开始和停止之间的时间的装置。 定时装置优选是以气动或发条为基础运行的定时装置，使得装置能够在没有任何电力供应的情况下操作，仅由吹扫气体压力驱动。 这使得能够构造简单可靠的单元，但是如果需要，甚至能够将该单元结合到容器本身中。 使用气动阀使得便携式设备可以被设计成具有低功率使用，如果它包括例如 用于测量从容器中排出的净化气体的性质的电子传感器。

公开(公告)号：US08642274B2

公开(公告)日：2014-02-04

申请号：US12503512

申请日：2009-07-15

Applicant: Richard Harrop ,  William Shingler ,  Stuart Naylor ,  Michael McDonald

Inventor： Richard Harrop ,  William Shingler ,  Stuart Naylor ,  Michael McDonald

IPC: G01N33/53

CPC classification number: G01N33/6854 ,  A61K39/0011 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  C12N2799/023 ,  G01N33/574 ,  G01N33/57419 ,  G01N33/57438 ,  G01N2800/52

Abstract: The present invention provides a method of monitoring the efficacy of an immunotherapy in a mammalian subject, wherein the subject has been administered an immunotherapy, wherein the immunotherapy comprises a viral vector containing a polynucleotide encoding an antigen, wherein the viral vector is capable of transducing cells in the mammalian subject to cause the cells to express the antigen; the method comprising: (b) measuring, from a biological sample isolated from the subject, an immune response of the subject to the antigen and comparing the immune response of the subject to the antigen to a reference measurement of immune response to the antigen; (c) measuring, from a biological sample isolated from the subject, an immune response of the subject to the viral vector and comparing the immune response of the subject to the viral vector to a reference measurement of immune response to the viral vector; and (d) determining efficacy based on the comparisons of (b) and (c), wherein an elevated immune response to the antigen and a reduced immune response to the viral vector are indicative of an effective immunotherapy.

Abstract translation: 本发明提供了监测哺乳动物受试者的免疫治疗功效的方法，其中所述受试者已经进行免疫治疗，其中所述免疫疗法包含含有编码抗原的多核苷酸的病毒载体，其中所述病毒载体能够转导细胞 在哺乳动物受试者中使细胞表达抗原; 该方法包括：（b）从分离自受试者的生物样品中测量受试者对抗原的免疫应答并将受试者与抗原的免疫应答与抗原的免疫应答的参考测量进行比较; （c）从分离自受试者的生物样品中测量受试者对病毒载体的免疫应答，并将受试者与病毒载体的免疫应答与对病毒载体的免疫应答的参考测量进行比较; 和（d）基于（b）和（c）的比较确定功效，其中对抗原的免疫应答升高和对病毒载体的免疫应答降低是指示有效的免疫治疗。

公开(公告)号：US07878112B2

公开(公告)日：2011-02-01

申请号：US11883296

申请日：2006-01-30

Applicant: Stuart Naylor

Inventor： Stuart Naylor

IPC: A23L1/00 ,  A23L3/34

CPC classification number: G01N1/2226 ,  B65D81/2076 ,  G01N2001/2241

Abstract: Apparatus is described for storing sensitive materials in containers (32) which are sealed and in which the internal atmosphere may be purged by flushing with an inert gas fed in from a purger unit (30) via a suitable feed lead (35). The gas exhausted from inside the container (32) is fed back into the purge unit (30) and then checked against a desired parameter, for example oxygen concentration, until the value of that parameter reaches a desired level. Purging may then be stopped and the container left sealed, preferably at a slight over pressure relative to the ambient atmosphere. The use of a separate purge unit enables many containers to be sequentially and effectively processed. The containers are particularly useful for storing sensitive chemical and biological samples.

Abstract translation: 描述了用于将敏感材料存储在容器（32）中的装置，所述容器（32）被密封，并且可以通过经由适当的进料引线（35）从净化器单元（30）进料的惰性气体冲洗来清洗内部气氛。 从容器（32）内部排出的气体被反馈到清洗单元（30）中，然后检查所期望的参数，例如氧浓度，直到该参数的值达到期望水平。 然后可以停止清洗，并且容器保持密封，优选地相对于环境气氛稍微过压。 使用单独的吹扫单元可以顺序地和有效地处理许多容器。 这些容器特别适用于储存敏感的化学和生物样品。

公开(公告)号：US20080125387A1

公开(公告)日：2008-05-29

申请号：US11705645

申请日：2007-02-12

Applicant: Katie Mary Binley ,  Susan Mary Kingsman ,  Stuart Naylor

Inventor： Katie Mary Binley ,  Susan Mary Kingsman ,  Stuart Naylor

IPC: A61K31/70 ,  C12N15/63 ,  A61P3/00

CPC classification number: C12N15/86 ,  A61K31/70 ,  A61K38/1816 ,  A61K48/00 ,  C07K14/505 ,  C12N2750/14143 ,  C12N2799/025 ,  C12N2830/002 ,  C12N2830/008

Abstract: Disclosed is a viral vector containing a nucleic acid sequence encoding erythropoietin (Epo), in operable linkage with an HRE expression control sequence, as well as uses of the vector; for instance, in preparing a medicament. Also provided are methods for treating anemia, can involve administering the vector to a patient, wherein expression of Epo is physiologically regulated such that hematocrit levels of the patient are corrected and maintained.

Abstract translation: 公开了含有编码红细胞生成素（Epo）的核酸序列的病毒载体，其与HRE表达控制序列可操作地连接，以及载体的用途; 例如，在制备药物中。 还提供了治疗贫血的方法，可以涉及向患者施用载体，其中Epo的表达在生理学上被调节，使得患者的血细胞比容水平被校正和维持。