公开(公告)号：US06656354B2

公开(公告)日：2003-12-02

申请号：US10066955

申请日：2002-02-04

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III ,  Kenneth J. Klein

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III ,  Kenneth J. Klein

IPC分类号： B01D1508

CPC分类号： B01D15/247 ,  B01D11/0203 ,  B01D15/40 ,  G01N1/405 ,  G01N30/24 ,  G01N30/82

摘要： A fractionated sample collection process and device having at least one pressurized chamber used to gather and store liquid samples generated from a flow stream containing a mixture of highly compressed gas, compressible liquid, or supercritical fluid and a relatively incompressible liquid. A bank of multiple collection chambers is secured together in a frame to form a cassette unit. Each collection chamber may house a replaceable liner such as a test tube vial, to hold a fractionated liquid phase sample. After filling with sample, a collection chamber is returned to a clean state and ready for refilling with a new sample by manually or automatically replacing the liner.

公开(公告)号：US06558540B2

公开(公告)日：2003-05-06

申请号：US09917210

申请日：2001-07-30

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

IPC分类号： B01D1508

CPC分类号： B01D11/0203 ,  B01D15/247 ,  B01D15/40 ,  G01N1/405 ,  G01N30/24 ,  G01N30/82

摘要： An efficient and safe method and apparatus for collecting exhaust gasses in an open-air sample collection container in a supercritical fluid chromatography system is claimed. The invention uses a vacuum force to evacuate the gaseous phase and vapors separated from liquid mobile phase in a sample collection device. The invention is portable to use on a series of collection containers, such as test tubes or wells in a titration place. Embodiments are claimed for capture of gasses and vapors either internally or externally to a collection container. The invention removes the gasses from the collection container that are then safely vented to a waste collector, thereby creating a safer environment for the laboratory and properly removing and disposing potentially hazardous chemicals instead of venting these chemicals into the environment.

摘要翻译： 一种用于在超临界流体色谱系统中的露天样品收集容器中收集废气的有效和安全的方法和装置。 本发明使用真空力来抽空样品收集装置中的气相和与液体流动相分离的蒸汽。 本发明便携式用于一系列收集容器，例如滴定处的试管或孔。 要求用于在收集容器的内部或外部捕集气体和蒸气的实施例。 本发明从收集容器中除去气体，然后将其安全地排放到废物收集器，从而为实验室创造更安全的环境，并适当地去除和处置潜在的危险化学品，而不是将这些化学物质排放到环境中。

公开(公告)号：US06685828B2

公开(公告)日：2004-02-03

申请号：US10119529

申请日：2002-04-09

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  Kenneth Klein ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  Kenneth Klein ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

IPC分类号： B01D1508

CPC分类号： B01D11/0203 ,  B01D15/247 ,  B01D15/40 ,  G01N30/24 ,  G01N30/82

摘要： A system for collection of liquid phase sample fractions from a supercritical fluid chromatography system is disclosed. The system has different ways of gathering the liquid phase into a collection chamber and provides for automated removal of liquid phase from a collection chamber into separate collection container. A separate pressure scheme is invoked upon the collection chambers to move liquid phase out of a chamber and into the separate collection container. In addition, the invention provides a renewal system for the collection chambers where each chamber returns to an uncontaminated stated by washing with a cleaning solution after removal of collected liquid phase. Spent cleaner from a chamber is purged using the pressure system into a waste stream for disposal.

摘要翻译： 公开了一种用于从超临界流体色谱系统收集液相样品馏分的系统。 该系统具有将液相收集到收集室中并且提供将液相从收集室自动移除到单独的收集容器中的不同方式。 在收集室上引用单独的压力方案以将液相移出室并进入单独的收集容器。 此外，本发明提供了一种用于收集室的更新系统，其中每个室在去除收集的液相后通过用清洁溶液洗涤而返回到未污染的状态。 使用压力系统将来自室的废清洁剂清洗成废物流以便处理。

公开(公告)号：US06648609B2

公开(公告)日：2003-11-18

申请号：US10117984

申请日：2002-04-05

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  Kenneth Klein ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  Kenneth Klein ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

IPC分类号： F04B4908

CPC分类号： F04B11/0091 ,  F04B49/225 ,  Y10T137/7838 ,  Y10T137/87917 ,  Y10T137/88054 ,  G01N2030/326 ,  B01D15/40

摘要： The invention is a device and method in a high-pressure chromatography system, such as a supercritical fluid chromatography (SFC) system, that uses a pump as a pressure source for precision pumping of a compressible fluid. The preferred exemplary embodiment comprises a pressure regulation assembly installed downstream from a compressible fluid pump but prior to combining the compressible flow with a relatively incompressible modifier flow stream. The present invention allows the replacement of an high-grade SFC pump in the compressible fluid flow stream with an inexpensive and imprecise pump. The imprecise pump becomes capable of moving the compressible fluid flow stream in a precise flow rate and pattern. The assembly dampens the damaging effects of an imprecise pump, such as large pressure oscillations caused by flow ripples and noisy pressure signals that do not meet precise SFC pumping requirements.

摘要翻译： 本发明是高压色谱系统中的装置和方法，例如超临界流体色谱（SFC）系统，其使用泵作为精密泵送可压缩流体的压力源。 优选的示例性实施例包括安装在可压缩流体泵的下游但在将可压缩流与相对不可压缩的修饰剂流动流组合之前的压力调节组件。 本发明允许用便宜且不精确的泵替代可压缩流体流动中的高级SFC泵。 不精确的泵变得能够以精确的流速和模式移动可压缩流体流动流。 该组件会抑制不精确泵的破坏性影响，例如由流动波纹引起的大压力振荡和不符合精确SFC泵送要求的噪声压力信号。

公开(公告)号：US07048517B2

公开(公告)日：2006-05-23

申请号：US10464333

申请日：2003-06-18

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  Kenneth Klein ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  Kenneth Klein ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

IPC分类号： F04B49/03

CPC分类号： F04B11/0091 ,  F04B49/225 ,  Y10T137/7838 ,  Y10T137/87917 ,  Y10T137/88054 ,  G01N2030/326 ,  B01D15/40

摘要： The invention is a device and method in a high-pressure chromatography system, such as a supercritical fluid chromatography (SFC) system, that uses a pump as a pressure source for precision pumping of a compressible fluid. The preferred exemplary embodiment comprises a pressure regulation assembly installed downstream from a compressible fluid pump but prior to combining the compressible flow with a relatively incompressible modifier flow stream. The present invention allows the replacement of an high-grade SFC pump in the compressible fluid flow stream with an inexpensive and imprecise pump. The imprecise pump becomes capable of moving the compressible fluid flow stream in a precise flow rate and pattern. The assembly dampens the damaging effects of an imprecise pump, such as large pressure oscillations caused by flow ripples and noisy pressure signals that do not meet precise SFC pumping requirements.

公开(公告)号：US06632353B2

公开(公告)日：2003-10-14

申请号：US10113599

申请日：2002-03-29

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III ,  Kenneth Klein

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III ,  Kenneth Klein

IPC分类号： B01D1508

CPC分类号： B01D11/0203 ,  B01D15/247 ,  B01D15/40 ,  G01N1/18 ,  G01N1/405 ,  G01N30/24 ,  G01N30/82 ,  Y10T436/255 ,  Y10T436/2575

摘要： A process and device for chromatography, such as preparative and analytical supercritical fluid chromatography, or supercritical fluid extraction for a liquid phase SFC collection system that separates and retains eluted components from sample injections. The process efficiently separates liquid and gas phases in the flow stream upstream of a collection vessel and without additional pressure schemes or solvent extractions imposed on the flowstream. Sample fractions are collected from sample component concentration peaks based upon the chromatographic elution of the sample components. Samples are repeatedly the injected into the mobile phase flow stream and collected into large volume collection containers, thereby allowing longer unattended run times and cost-efficient sample purification and recovery.

摘要翻译： 用于层析的方法和装置，例如制备和分析超临界流体色谱法或用于液相SFC收集系统的超临界流体萃取，其从样品注射中分离并保留洗脱的组分。 该方法有效地分离收集容器上游的流动流中的液相和气相，而没有施加在流动流上的额外的压力方案或溶剂萃取。 基于样品组分的色谱洗脱，从样品组分浓度峰中收集样品级分。 将样品重复注入流动相流中并收集到大容量收集容器中，从而允许更长的无人值守运行时间和经济高效的样品纯化和回收。

公开(公告)号：US06561767B2

公开(公告)日：2003-05-13

申请号：US09918436

申请日：2001-08-01

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats, III ,  Mark Nickerson ,  Paul F. Bente, III

IPC分类号： F04B1924

CPC分类号： G01N30/32 ,  G01N30/36 ,  G01N2030/326 ,  G01N30/02 ,  B01D15/40

摘要： A method for converting a pump for use in a flow stream containing a mixture of highly compressed gas, compressible liquid or supercritical fluid wherein the pump delivers a compressible fluid against a back-pressure regulator which in turn delivers a controllable flow rate downstream of the regulator without performing variable compressibility compensation adjustments on the pump. By using a pressurized source of compressible fluid combined with isocratic conditions, the delivery pressure from the pump is controlled at a rate higher than the downstream gradient with the back-pressure regulator, and an inexpensive pump may be used in place of specialized, expensive pumps and compressibility compensation systems for use in stems operating at or near supercritical fluid levels of compressible fluids. Significant capital and operating laboratory costs are saved through a simpler and cheaper system for accurately delivering compressible fluids into mobile phase flow streams.

摘要翻译： 一种用于转换用于包含高压缩气体，可压缩液体或超临界流体的混合物的流动流的泵的方法，其中所述泵将可压缩流体递送抵靠背压调节器，所述背压调节器又传递调节器下游的可控流量 不对泵进行可变的压缩补偿调整。 通过使用与等度条件相结合的可压缩流体的加压源，来自泵的输送压力以比背压调节器高的下游梯度的速度被控制，并且可以使用廉价的泵来代替专门的，昂贵的泵 以及用于在可压缩流体的超临界流体水平处或附近操作的茎中的压缩性补偿系统。 通过更简单和更便宜的系统节省大量资本和操作实验室成本，将可压缩流体精确传递到流动相流动流中。

公开(公告)号：US06652753B2

公开(公告)日：2003-11-25

申请号：US10067011

申请日：2002-02-04

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats ,  Mark Nickerson ,  Paul Bente ,  Kenneth J. Klein

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats ,  Mark Nickerson ,  Paul Bente ,  Kenneth J. Klein

IPC分类号： C02F144

CPC分类号： B01D15/247 ,  B01D11/0203 ,  B01D15/40 ,  G01N1/405 ,  G01N30/24 ,  G01N30/82

摘要： A fractionated sample collection process and device having at least one pressurized chamber used to gather and store liquid samples generated from a flow stream containing a mixture of highly compressed gas, compressible liquid, or supercritical fluid and a relatively incompressible liquid. A bank of multiple collection chambers is secured together in a frame to form a cassette unit. Each collection chamber may house a replaceable liner such as a test tube vial, to hold a fractionated liquid phase sample. After filling with sample, a collection chamber is returned to a clean state and ready for refilling with a new sample by manually or automatically replacing the liner.

公开(公告)号：US06413428B1

公开(公告)日：2002-07-02

申请号：US09607316

申请日：2000-06-26

申请人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats ,  Mark Nickerson ,  Paul Bente

发明人： Terry A. Berger ,  Kimber D. Fogelman ,  L. Thompson Staats ,  Mark Nickerson ,  Paul Bente

IPC分类号： B01D1508

CPC分类号： B01D15/247 ,  B01D11/0203 ,  B01D15/40 ,  G01N1/405 ,  G01N30/24 ,  G01N30/82

摘要： A fractionated sample collection process and device having at least one pressurized chamber used to gather and store liquid samples generated from a flow stream containing a mixture of highly compressed gas, compressible liquid, or supercritical fluid and a relatively incompressible liquid. A bank of multiple collection chambers is secured together in a frame to form a cassette unit. Each collection chamber may house a replaceable liner such as a test tube vial, to hold a fractionated liquid phase sample. After filling with sample, a collection chamber is returned to a clean state and ready for refilling with a new sample by manually or automatically replacing the liner.

摘要翻译： 分级样品收集过程和装置具有至少一个加压室，用于收集和储存从含有高度压缩气体，可压缩液体或超临界流体和相对不可压缩液体的混合物的流动流产生的液体样品。 多个收集室的一组在框架中固定在一起以形成盒单元。 每个收集室可以容纳诸如试管小瓶的可更换的衬里，以容纳分馏的液相样品。 填充样品后，收集室返回到清洁状态，并通过手动或自动更换衬垫，准备用新样品进行再填充。