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公开(公告)号：US09677052B2
公开(公告)日：2017-06-13
申请号：US14707718
申请日：2015-05-08
CPC分类号： A61K39/39 , A61K2039/55516 , A61K2039/55572 , A61K2039/55583 , A61K2039/55594 , C07H13/06 , C07H15/04 , C07K4/00 , C12N9/00 , C12N9/93 , C12N15/04 , C12P21/005
摘要： The present disclosure generally relates to genetic engineering of bacteria. More particularly, the present disclosure describes genetic engineering of E. coli to create mutant O-antigen ligase, as well as novel lipopolysaccharide molecules resulting from that genetic engineering. Methods for using those novel molecules are also described.
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公开(公告)号：US20150320857A1
公开(公告)日：2015-11-12
申请号：US14707718
申请日：2015-05-08
CPC分类号： A61K39/39 , A61K2039/55516 , A61K2039/55572 , A61K2039/55583 , A61K2039/55594 , C07H13/06 , C07H15/04 , C07K4/00 , C12N9/00 , C12N9/93 , C12N15/04 , C12P21/005
摘要： The present disclosure generally relates to genetic engineering of bacteria. More particularly, the present disclosure describes genetic engineering of E. coli to create mutant O-antigen ligase, as well as novel lipopolysaccharide molecules resulting from that genetic engineering. Methods for using those novel molecules are also described.
摘要翻译： 本公开一般涉及细菌的遗传工程。 更具体地，本公开描述了大肠杆菌的基因工程以产生突变的O-抗原连接酶，以及由该基因工程产生的新的脂多糖分子。 还描述了使用这些新型分子的方法。
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公开(公告)号：US4336336A
公开(公告)日：1982-06-22
申请号：US3082
申请日：1979-01-12
CPC分类号： C12N15/62
摘要： This invention is a method for fusing, within a bacterial host, a gene for a cytoplasmic protein to a gene for a non-cytoplasmic protein, so that the hybrid protein produced is transported to, near, or beyond the cell surface for ease of collection and purification, or for immunological use. The invention also encompasses the fused gene created by the disclosed method.
摘要翻译： 本发明是在细菌宿主中将细胞质蛋白质的基因融合到非细胞质蛋白质的基因的方法，使得所产生的杂交蛋白质被运送到细胞表面附近或超出细胞表面，以便于收集 和纯化，或用于免疫学应用。 本发明还包括通过所公开的方法产生的融合基因。
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公开(公告)号：US4503142A
公开(公告)日：1985-03-05
申请号：US392308
申请日：1982-06-25
IPC分类号： C12N15/64 , G01N33/68 , C12P21/00 , C07H21/04 , C12N1/00 , C12N1/20 , C12N15/00 , C12P19/34 , C12P21/02 , C12Q1/00 , C12Q1/34 , C12Q1/68
CPC分类号： G01N33/68 , C12N15/64 , Y10S436/804 , Y10S930/31
摘要： An open reading frame DNA vector is presented having a promoter-translation start region, and adjacent thereto and downstream therefrom, a first coding segment having a start codon, a second coding segment coding for detectable protein, and an insertion region between said first and second coding regions having an insertion site and being of such length that the first and second coding segments are not in reading frame phase with each other. A method for the cloning and positive selection of a DNA segment having an open reading frame is also presented, as well as a tribrid protein wherein each terminal region and the mid-section is derived from a different gene, and a method of identifying and quantifying a protein or polypeptide without regard to its structure or biological function by using a DNA segment coding for said protein or polypeptide as the mid-section of the tribrid protein.
摘要翻译： 呈现开放阅读框DNA载体，其具有启动子 - 翻译起始区，并且与其相邻并且在其下游，具有起始密码子的第一编码区段，编码可检测蛋白质的第二编码区段和所述第一和第二区域之间的插入区域 具有插入位置并且具有第一和第二编码段不处于读取帧相位的长度的编码区域。 还提出了具有开放阅读框的DNA片段的克隆和阳性选择的方法，以及其中每个末端区域和中间片段来自不同基因的三联蛋白质，以及鉴定和定量的方法 不考虑其结构或生物功能的蛋白质或多肽，通过使用编码所述蛋白质或多肽的DNA区段作为三链体蛋白质的中间区段。
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