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    Collagen-binding proteins from enterococcal bacteria
    6.
    发明授权
    Collagen-binding proteins from enterococcal bacteria 有权
    来自肠球菌的胶原蛋白结合蛋白

    公开(公告)号：US06908994B1

    公开(公告)日：2005-06-21

    申请号：US09568470

    申请日：2000-05-10

    申请人： Rebecca L. Rich ,  Bernd Kriekemeyer ,  Rick T. Owens ,  Magnus Hook ,  Barbara E. Murray ,  Sreedhar R. Nallapareddy ,  George M. Weinstock

    发明人： Rebecca L. Rich ,  Bernd Kriekemeyer ,  Rick T. Owens ,  Magnus Hook ,  Barbara E. Murray ,  Sreedhar R. Nallapareddy ,  George M. Weinstock

    IPC分类号： A61K39/02 ,  C07K16/12 ,  G01N33/569 ,  C07H21/04 ,  A61K39/00 ,  C07H21/02

    CPC分类号： G01N33/56938 ,  A61K39/02 ,  A61K2039/505 ,  C07K16/1271 ,  C07K16/1275 ,  G01N2333/315

    摘要： A collagen-binding MSCRAMM entitled Ace from enterococcal bacteria is provided which was homologous to the ligand-binding region of Cna, the collagen-binding MSCRAMM from Staphylococcus aureus, and which can be utilized in a similar manner as other collagen-binding MSCRAMMs to inhibit adhesion of enterococcal bacteria to extracellular matrix proteins. The N-terminal region of Ace contained a region (residues 174-319), or A domain, which appears to be equivalent to the minimal ligand-binding region of the collagen-binding protein Cna (Cna 151-318), and contains several 47-residue tandem repeat units, called B domain repeat units, between the collagen-binding site and cell wall-associated regions. The Ace protein of the invention can thus be utilized in methods of preventing and/or treating enterococcal infection, and in addition, antibodies raised against Ace, or its A domain, can be used to effectively inhibit the adhesion of enterococcal cells to a collagen substrate. The Ace protein of the present invention is thus a functional collagen-binding MSCRAMM and can be utilized to treat or prevent invention in the same manner as other isolated MSCRAMMs have been utilized, namely in methods of treating or preventing infections and diseases caused by enterococcal bacteria.

    摘要翻译： 提供了一种与来自肠球菌的Ace的胶原结合的MSCRAMM，其与来自金黄色葡萄球菌的胶原结合MSCRAMM的Cna的配体结合区同源，并且可以以与其它胶原结合的MSCRAMM类似的方式用于抑制 肠球菌对细胞外基质蛋白的粘附。 Ace的N末端区域包含一个似乎等同于胶原结合蛋白Cna（Cna 151-318）的最小配体结合区域的区域（残基174-319）或A结构域，并且包含几个 在胶原结合位点和细胞壁相关区域之间的称为B结构域重复单元的47个残基串联重复单元。 因此，本发明的Ace蛋白质可用于预防和/或治疗肠球菌感染的方法，此外，针对Ace或其A结构域产生的抗体可用于有效抑制肠球菌细胞与胶原底物的粘附 。 因此，本发明的Ace蛋白是功能性胶原结合MSCRAMM，并且可以以与其它已分离的MSCRAMM相同的方式用于治疗或预防发明，即治疗或预防由肠球菌引起的感染和疾病的方法 。