公开(公告)号：US06255061B1

公开(公告)日：2001-07-03

申请号：US09464613

申请日：1999-12-15

申请人： Toshihiro Mori ,  Takaki Arai ,  Yoshikazu Amano

发明人： Toshihiro Mori ,  Takaki Arai ,  Yoshikazu Amano

IPC分类号： C12Q154

CPC分类号： G01N33/525 ,  C12Q1/26 ,  C12Q1/28 ,  Y10S435/966

摘要： An analytical element for quantitative analysis of glucose, cholesterol, or lactic acid in whole blood, composed of a porous layer which has a void volume of 30 to 80% and containing peroxidase, a leuco dye which gives coloring having an absorption peak in the region of 600 to 700 nm, and an oxidase such as glucose oxidase, cholesterol oxidase, or lactate oxidase, respectively. The porous layer can be placed on a transparent support sheet via an adhesive layer in the form of dots or lines, or a hydrophilic adhesive layer.

摘要翻译： 用于定量分析全血中的葡萄糖，胆固醇或乳酸的分析元件，其由空隙体积为30至80％的多孔层和含有过氧化物酶的无机染料组成，所述无色染料在所述区域中具有吸收峰 分别为600〜700nm，氧化酶如葡萄糖氧化酶，胆固醇氧化酶或乳酸氧化酶。 多孔层可以通过粘合剂层以点或线的形式或亲水性粘合剂层放置在透明支撑片上。

公开(公告)号：US20060024770A1

公开(公告)日：2006-02-02

申请号：US11230127

申请日：2005-09-19

申请人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

发明人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

IPC分类号： G01N33/537 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/54386 ,  G01N2333/924 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

公开(公告)号：US5709837A

公开(公告)日：1998-01-20

申请号：US545233

申请日：1995-10-19

申请人： Toshihiro Mori ,  Masashi Ogawa ,  Yoshikazu Amano

发明人： Toshihiro Mori ,  Masashi Ogawa ,  Yoshikazu Amano

IPC分类号： G01N31/22 ,  G01N33/52 ,  G01N33/70 ,  G01N21/17

CPC分类号： G01N33/525 ,  Y10T436/147777 ,  Y10T436/171538

摘要： A dry analytical element comprising at least one water-permeable reagent layer and a porous spreading layer laminated in this order on a support, wherein at least one layer which is the water-permeable reagent layer, the porous spreading layer or an optional water-permeable layer contains a water-soluble polybasic amino carboxylic acid which is an ampholyte. By applying an analytical element of the invention, an analyte can be measured at a high sensitivity using a small amount of an enzyme, etc., and shelf file can be extended.

摘要翻译： 一种干燥分析元件，其包括依次层叠在载体上的至少一个透水性试剂层和多孔扩散层，其中至少一层作为透水性试剂层，多孔扩散层或任选的透水性 层含有两性电解质的水溶性多元氨基羧酸。 通过应用本发明的分析元件，可以使用少量的酶等以高灵敏度测量分析物，并且可以延长搁架文件。

公开(公告)号：US20050084953A1

公开(公告)日：2005-04-21

申请号：US10961459

申请日：2004-10-07

申请人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

发明人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

IPC分类号： G01N33/543 ,  G01N33/53

CPC分类号： G01N33/54386 ,  G01N2333/924 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

摘要翻译： 用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。 免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 作为非扩散性底物，可以使用能够仅与前体酶反应并且不能使片段化酶反应的底物。 当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中 是指具有对内源性葡糖苷酶特异性的反应性的底物。 高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

公开(公告)号：US06659288B2

公开(公告)日：2003-12-09

申请号：US09858391

申请日：2001-05-16

申请人： Yoshikazu Amano ,  Toshihiro Mori

发明人： Yoshikazu Amano ,  Toshihiro Mori

IPC分类号： B01D3500

CPC分类号： B01D39/2017

摘要： A plasma- or serum-collecting device with simple structure and easy to handle is provided. The device comprises a vacuum blood-drawing tube accommodating a blood-filtering unit, wherein the vacuum tube contains a space above the blood-filtering unit to preserve blood to be filtered and a partition wall under the blood-filtering unit to keep a backspace at reduced pressure, and the blood-filtering unit contains a communicating needle to break the partition wall at the bottom.

摘要翻译： 提供了一种结构简单，易于操作的等离子体或血清采集装置。 所述装置包括容纳血液净化单元的真空吸血管，其中所述真空管包含所述血液过滤单元上方的空间，以保持要过滤的血液和所述血液净化单元下方的分隔壁以保持退格 减压，血液滤过单元含有连通针，以破坏底部的分隔壁。

公开(公告)号：US07820402B2

公开(公告)日：2010-10-26

申请号：US11230127

申请日：2005-09-19

申请人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

发明人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

IPC分类号： G01N33/53 ,  G01N33/543

CPC分类号： G01N33/54386 ,  G01N2333/924 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

摘要翻译： 用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。 免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 作为非扩散性底物，使用能够仅与标记酶反应并且不能使片段化酶反应的底物。 当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中 是指具有对内源性葡糖苷酶特异性的反应性的底物。 高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

公开(公告)号：US07025212B2

公开(公告)日：2006-04-11

申请号：US10463987

申请日：2003-06-18

申请人： Yoshikazu Amano ,  Toshihiro Mori

发明人： Yoshikazu Amano ,  Toshihiro Mori

IPC分类号： B01D35/00

CPC分类号： B01D39/2017

摘要： A plasma- or serum-collecting device with simple structure and easy to handle is provided. The device comprises a vacuum blood drawing tube accommodating a blood-filtering unit, wherein the vacuum tube contains a space above the blood-filtering unit to preserve blood to be filtered and a partition wall under the blood-filtering unit to keep a backspace at reduced pressure, and the blood-filtering unit contains a communicating needle to break the partition wall at the bottom.

摘要翻译： 提供了一种结构简单，易于操作的等离子体或血清采集装置。 该装置包括容纳血液净化单元的真空吸血管，其中真空管在血液过滤单元上方包含一个空间，以保持待过滤的血液和血液净化单元下面的分隔壁以使退避空间减小 压力，并且血液过滤单元包含连通针以破坏底部的分隔壁。

公开(公告)号：US06659975B2

公开(公告)日：2003-12-09

申请号：US09858393

申请日：2001-05-16

申请人： Yoshikazu Amano ,  Toshihiro Mori

发明人： Yoshikazu Amano ,  Toshihiro Mori

IPC分类号： A61M3100

CPC分类号： A61B5/150488 ,  A61B5/15003 ,  A61B5/150213 ,  A61B5/150351 ,  A61B5/150389 ,  A61B5/150732 ,  A61B5/150755 ,  A61B5/154

摘要： A plasma-collecting devise comprises a blood-filtering element having a blood-drawing needle and a communicating needle, a vacuum blood-drawing tube, and a contracting and expanding element connecting the blood-filtering unit and the vacuum blood-drawing tube. The device enables blood drawing from blood vessel and blood filtering continuously to obtain plasma.

摘要翻译： 等离子体收集装置包括具有抽血针和连通针的血液过滤元件，真空吸血管，以及连接血液净化单元和真空吸血管的收缩和膨胀元件。 该装置使血液进行抽血，连续进行血液过滤，得到血浆。

公开(公告)号：US08658758B2

公开(公告)日：2014-02-25

申请号：US10593230

申请日：2005-03-17

申请人： Hiroyuki Sato ,  Fuminori Kobayashi ,  Yukichika Kawakami ,  Kazuyuki Yamane ,  Yoshikazu Amano ,  Takashi Sato

发明人： Hiroyuki Sato ,  Fuminori Kobayashi ,  Yukichika Kawakami ,  Kazuyuki Yamane ,  Yoshikazu Amano ,  Takashi Sato

IPC分类号： C08G63/08

CPC分类号： C08G63/90 ,  C08G63/06 ,  C08G63/80

摘要： In the production of an aliphatic polyester by ring-opening polymerization of a cyclic ester, at least a latter period of polymerization is proceeded with by way of solid-phase polymerization, and the resultant aliphatic polyester is subjected to removal of residual cyclic ester. As a result, an aliphatic polyester with a minimized content of residual monomer is obtained.

摘要翻译： 在通过环状酯的开环聚合生产脂肪族聚酯时，至少通过固相聚合进行聚合的后期，并将所得的脂族聚酯除去残留的环状酯。 结果，得到残留单体含量最小的脂肪族聚酯。

公开(公告)号：US5592290A

公开(公告)日：1997-01-07

申请号：US358486

申请日：1994-11-14

申请人： Fuminori Arai ,  Yoshikazu Amano ,  Kiyoshi Yamada

发明人： Fuminori Arai ,  Yoshikazu Amano ,  Kiyoshi Yamada

IPC分类号： G01N33/52 ,  G01N21/27 ,  G01N21/78 ,  G01N21/86 ,  G01N31/22 ,  G01J3/00

CPC分类号： G01N21/8483 ,  G01N21/274

摘要： A method for correcting an instrumental error due to wavelength error of a spectroscope of an optical analyzer for measuring an optical density reflected from or transmitted through a dry analysis element. One standard color plate having a standard optical density OD.sub.ST is measured by an optical analyzer to be corrected to obtain an measured value OD.sub.M. A measured optical density OD.sub.S of the element applied with a sample through the optical analyzer to be corrected is corrected by using a ratio of OD.sub.M /OD.sub.ST to obtain a corrected measured value OD.sub.C of the sample. The standard color plate to be used contains an indicator dye contained in the non-reacted dry analysis element or a dye having an absorption spectrum same as or similar to that of the indicator dye in the measurement wavelength range. Another correction method in consideration of a layer coefficient of a dry analysis element is also provided.

摘要翻译： 一种用于校正由用于测量从干分析元件反射或透过干光分析元件的光密度的光学分析仪的分光镜引起的波长误差的仪器误差的方法。 通过光学分析器测量具有标准光密度ODST的一个标准色板以进行校正，以获得测量值ODM。 通过使用ODM / ODST的比例来校正通过待校正的光学分析器施加样品的元件的测量光密度ODS，以获得样品的校正测量值ODC。 所使用的标准色板包含未反应干燥分析元件中所含的指示剂染料或在测量波长范围内具有与指示剂染料相同或类似的吸收光谱的染料。 还提供了考虑干分析元件的层系数的另一校正方法。